发明授权
- 专利标题： Recombinant DNA methods vectors and host cells
- 专利标题（中）： 重组DNA方法载体和宿主细胞
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申请号： US376380申请日： 1995-01-23
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公开(公告)号： US5891693A公开(公告)日： 1999-04-06
- 发明人: Christopher Robert Bebbington , Geoffrey Thomas Yarranton , Richard H. Wilson
- 申请人： Christopher Robert Bebbington , Geoffrey Thomas Yarranton , Richard H. Wilson
- 申请人地址： CHX Neuhausen am Rheinfall
- 专利权人： Alusuisse Holdings A.G.
- 当前专利权人： Alusuisse Holdings A.G.
- 当前专利权人地址： CHX Neuhausen am Rheinfall
- 主分类号： C07K14/025
- IPC分类号： C07K14/025 ; C07K14/045 ; C12N9/00 ; C12N15/85 ; C12N15/00 ; C07H21/02 ; C12P21/04 ; C12P21/06
摘要：
The present invention relates to vectors useful for transforming a lymphoid cell line to glutamine independence. The vectors comprise an active glutamine synthetase (GS) gene as well as a heterologous gene of interest to be expressed. The preferred embodiments encompass vectors wherein the heterologous gene is expressed from a relatively strong promoter and the GS gene is expressed from a relatively weak promoter. In one example, the heterologous gene is operatively linked to the hCMV-MIE promoter and the GS gene is operatively linked to the SV40 early region promoter.
公开/授权文献
- US6105926A Diaphragm assembly disc 公开/授权日：2000-08-22
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