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1.发明授权
公开(公告)号：US5871999A
公开(公告)日：1999-02-16
申请号：US355103
申请日：1994-12-13
CPC分类号： C12N5/0018 , C12N2500/05 , C12N2500/12
摘要： Adherent animal cells are grown in suspension in a nutrient medium in which the molar ratio of total inorganic ions and total amino acids is reduced when compared with the corresponding ratio in known nutrient media, and is maintained at a level at which little or no cell aggregation occurs. Further, the nutrient medium contains 10:1 to about 1:1 molar ratio of total inorganic ions to total amino acids. In one further embodiment, the nutrient medium contains a total sodium ion concentration in the range of 75 to 120 mM (millimole), a total chloride ion concentration in the range of 50 to 90 mM and a total amino acid content of 20 to 50 mM. The adherent animal cells include mammalian cells such as cells belonging to a human, rat, mouse or hamster. Chinese Hamster Ovary Cells (CHO cells) are cultured using the process of the disclosed invention.
摘要翻译： 粘附动物细胞在与已知营养培养基中的相应比例相比较下降的总无机离子与总氨基酸的摩尔比在营养培养基中的悬浮液中生长，并保持在很少或没有细胞聚集的水平 发生。 此外，营养培养基含有10:1至约1:1的总无机离子与总氨基酸的摩尔比。 在一个另外的实施方案中，营养培养基含有在75至120mM（毫摩尔）范围内的总钠离子浓度，总氯离子浓度在50至90mM范围内，总氨基酸含量为20至50mM 。 贴壁动物细胞包括哺乳动物细胞，例如属于人，大鼠，小鼠或仓鼠的细胞。 使用本发明的方法培养中国仓鼠卵巢细胞（CHO细胞）。
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公开(公告)号：US5811526A
公开(公告)日：1998-09-22
申请号：US447847
申请日：1995-05-23
IPC分类号： C09K11/06 , G01N33/533 , G01N33/553 , G01N33/58 , E07K16/00 , G01N33/532
CPC分类号： G01N33/553 , C09K11/06 , G01N33/533 , G01N33/582 , Y10S436/80 , Y10S436/805
摘要： A time-resolving luminescence binding assay involves a metal-labelled binding partner comprising a luminescent transition metal complex covalently attached to a binding partner such as an antigen or antibody. Ruthenim, iridium, osmium, and chromium luminescent complexes are described.
摘要翻译： 时间分辨发光结合测定涉及包含共价连接到结合配偶体（例如抗原或抗体）上的发光过渡金属络合物的金属标记的结合配偶体。 钌，铱，锇和铬发光配合物。
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公开(公告)号：US6117652A
公开(公告)日：2000-09-12
申请号：US856249
申请日：1997-05-14
申请人： Raymond Paul Field
发明人： Raymond Paul Field
IPC分类号： C07K14/52 , C07K14/61 , C07K16/00 , C07K16/06 , C07K16/34 , C12N1/38 , C12N5/16 , C12N9/72 , C12P21/04 , C12N15/09 , A61K35/14 , C12N5/12
CPC分类号： C12N9/6459 , C07K14/52 , C07K14/61 , C07K16/00 , C07K16/065 , C07K16/34 , C12N1/38 , C12N5/163 , C12Y304/21069 , C12N2500/36
摘要： Methods for enhancing the production of tissue plasminogen activator (tPA) in cell culture are disclosed. The methods involve culturing tPA-producing cells in growth media supplemented with an alkanoic acid or salt thereof at a concentration which enhances tPA production. The most preferred methods utilize butyric acid or sodium butyrate at a concentration of between 0.1 mM and 10 mM.
摘要翻译： 公开了增强细胞培养物中组织纤溶酶原激活物（tPA）生产的方法。 所述方法包括以增强tPA产生的浓度培养补充有链烷酸或其盐的生长培养基中的tPA产生细胞。 最优选的方法是使用浓度为0.1mM至10mM的丁酸或丁酸钠。
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公开(公告)号：US5866359A
公开(公告)日：1999-02-02
申请号：US442646
申请日：1995-05-16
CPC分类号： C12N15/85 , C12N2800/108
摘要： The present invention provides a process and host cell for use in increasing the amount of a desired protein produced by a cell line. The process and the cell line use a first transcription unit containing a gene for a transactivator protein to control the transactivation of a second transcription unit such that the amount of desired protein expression can be increased without affecting adversely cell growth. Preferred transactivator proteins are derived from E1A.
摘要翻译： 本发明提供了用于增加细胞系产生的所需蛋白质的量的方法和宿主细胞。 该方法和细胞系使用包含反式激活蛋白的基因的第一转录单位来控制第二转录单位的反式激活，使得可以增加所需蛋白质表达的量而不影响不利的细胞生长。 优选的反式激活蛋白衍生自E1A。
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公开(公告)号：US5874232A
公开(公告)日：1999-02-23
申请号：US979792
申请日：1997-11-26
CPC分类号： C12N9/0065 , C12N9/96 , C12Q1/28 , G01N33/535 , C12Q2326/12 , G01N2400/18 , Y10S435/975
摘要： We have discovered that the stability of a dilute peroxidase-containing solution is greatly enhanced by the addition of a specific substrate for the peroxidase, in the absence of peroxide. We provide a peroxidase-containing reagent consisting of a buffered aqueous solution comprising a peroxidase or a peroxidase conjugate and a specific substrate for the peroxidase, in the absence of peroxide. The peroxidase may be a free peroxidase but for assay purposes is preferably a peroxidase bound to a specific binding component of an assay, to form a peroxidase conjugate. The peroxidase conjugate is preferably a conjugate between a peroxidase and an antigen or an antibody, most preferably an antibody. The peroxidase is preferably horseradish peroxidase.
摘要翻译： 我们已经发现，在不存在过氧化物的情况下，通过添加过氧化物酶的特定底物，可以大大提高稀释过氧化物酶溶液的稳定性。 在不存在过氧化物的情况下，我们提供由含有过氧化物酶或过氧化物酶缀合物的缓冲水溶液和过氧化物酶特异性底物组成的含过氧化物酶的试剂。 过氧化物酶可以是游离过氧化物酶，但是为了测定目的优选是结合于测定的特异性结合组分的过氧化物酶，以形成过氧化物酶缀合物。 过氧化物酶缀合物优选是过氧化物酶与抗原或抗体之间的缀合物，最优选抗体。 过氧化物酶优选是辣根过氧化物酶。
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6.发明授权Transformed myeloma cell-line and a process for the expression of a gene coding for a eukaryotic polypeptide employing same 失效转化的骨髓瘤细胞系和使用其表达编码真核生物多肽的基因的方法
公开(公告)号：US5981216A
公开(公告)日：1999-11-09
申请号：US483813
申请日：1995-06-07
CPC分类号： C12N9/6459 , C12N15/85 , C12Y304/21069 , C12N2830/002 , C12N2830/85
摘要： A myeloma cell-line transformed with a vector including a gene coding for a eukariotic polypeptide and a non-immunolglobulin promoter such that expression occurs of the gene coding for the eukariotic polypeptide, directed by the non-immunoglobulin promoter. The promoter may be a viral promoter, such as an SV40 promoter, a Rous sarcoma virus long terminal repeat or a Moloney murine leukemia long terminal repeat, or a non-viral promoter such as the mouse metallothionein promoter. Rat and mouse host myeloma cell-lines such as the rat YB/2/3.0 Ag20 hybridoma, the mouse SP-20 Ag hybridoma and the mouse NSO hybridoma are employed. The production of tissue plasminogen activator (tPA) is exemplified.
摘要翻译： 用包含编码真核细胞多肽的基因和非免疫球蛋白启动子的载体转化的骨髓瘤细胞系，使得由非免疫球蛋白启动子指导的编码真核细胞多肽的基因的表达发生。 启动子可以是病毒启动子，例如SV40启动子，劳斯肉瘤病毒长末端重复或莫洛尼鼠白血病长末端重复序列，或非病毒启动子如小鼠金属硫蛋白启动子。 使用大鼠和小鼠宿主骨髓瘤细胞系如大鼠YB / 2 / 3.0Ag20杂交瘤，小鼠SP-20GG杂交瘤和小鼠NSO杂交瘤。 组织纤溶酶原激活物（tPA）的生产被列举。
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公开(公告)号：US5891693A
公开(公告)日：1999-04-06
申请号：US376380
申请日：1995-01-23
IPC分类号： C07K14/025 , C07K14/045 , C12N9/00 , C12N15/85 , C12N15/00 , C07H21/02 , C12P21/04 , C12P21/06
CPC分类号： C07K14/005 , C12N15/85 , C12N9/93 , C12N2710/16122 , C12N2710/22022
摘要： The present invention relates to vectors useful for transforming a lymphoid cell line to glutamine independence. The vectors comprise an active glutamine synthetase (GS) gene as well as a heterologous gene of interest to be expressed. The preferred embodiments encompass vectors wherein the heterologous gene is expressed from a relatively strong promoter and the GS gene is expressed from a relatively weak promoter. In one example, the heterologous gene is operatively linked to the hCMV-MIE promoter and the GS gene is operatively linked to the SV40 early region promoter.
摘要翻译： 本发明涉及用于将淋巴细胞系转化为谷氨酰胺独立性的载体。 载体包含活性谷氨酰胺合成酶（GS）基因以及待表达的感兴趣的异源基因。 优选的实施方案包括其中异源基因由相对强的启动子表达并且GS基因由相对弱的启动子表达的载体。 在一个实例中，异源基因与hCMV-MIE启动子有效连接，GS基因与SV40早期区域启动子有效连接。
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公开(公告)号：US5736353A
公开(公告)日：1998-04-07
申请号：US261007
申请日：1994-06-14
IPC分类号： C12N9/08 , C12N9/96 , C12Q1/28 , G01N33/535
CPC分类号： C12N9/0065 , C12N9/96 , C12Q1/28 , G01N33/535 , C12Q2326/12 , G01N2400/18 , Y10S435/975
摘要： The present invention relates to a peroxidase-containing reagent comprising a buffered aqueous solution of a peroxidase conjugate and a substrate for the peroxidase, in the absence of peroxide. The invention further relates to a method of stabilizing the peroxidase activity of a buffered aqueous solution containing a peroxidase conjugate. Further, the invention relates to a tetramethylbenzidine peroxidase substrate reagent and to a method of preparing same.
摘要翻译： 本发明涉及含有过氧化物酶的试剂，其在不存在过氧化物的情况下，包含过氧化物酶缀合物的缓冲水溶液和过氧化物酶底物。 本发明还涉及稳定含有过氧化物酶缀合物的缓冲水溶液的过氧化物酶活性的方法。 此外，本发明涉及四甲基联苯胺过氧化物酶底物试剂及其制备方法。
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