发明授权
US5928917A Conjugate of non-protein chemical moiety and polypeptide having
cellulose binding region
失效
具有纤维素结合区域的非蛋白质化学部分和多肽的缀合物
- 专利标题: Conjugate of non-protein chemical moiety and polypeptide having cellulose binding region
- 专利标题(中): 具有纤维素结合区域的非蛋白质化学部分和多肽的缀合物
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申请号: US249037申请日: 1994-05-24
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公开(公告)号: US5928917A公开(公告)日: 1999-07-27
- 发明人: Douglas G. Kilburn , Robert C. Miller , Neil Gilkes , R. Antony J. Warren
- 申请人: Douglas G. Kilburn , Robert C. Miller , Neil Gilkes , R. Antony J. Warren
- 申请人地址: CAX
- 专利权人: University of British Columbia
- 当前专利权人: University of British Columbia
- 当前专利权人地址: CAX
- 主分类号: G01N33/573
- IPC分类号: G01N33/573 ; A61K39/39 ; C07K14/55 ; C07K17/12 ; C07K19/00 ; C08B15/00 ; C08B37/08 ; C08H8/00 ; C12N9/16 ; C12N9/38 ; C12N9/42 ; C12N11/12 ; C12N15/09 ; C12P21/02 ; C12Q1/56 ; C12R1/19 ; D06P3/60 ; D21H17/00 ; G01N33/531 ; C12N15/00 ; C12P21/00
摘要:
Fusion proteins or conjugates are provided containing an amino acid sequence having a substrate binding region of a polysaccharidase such as cellulase that binds to a .beta.-1,4-glycan matrix such as cellulose. The substrate binding region is essentially without polysaccharidase activity. In the fusion protein, the substrate binding region is fused or chemically linked to a polypeptide such as an enzyme, a hormone, an immunoglobulin or a protein dye. By contacting the fusion protein with a .beta.-1,4-glycan matrix, the substrate binding region binds to the matrix to immobilize the polypeptide on the matrix. The polypeptide or fusion protein can be removed from the matrix with a protease recognition sequence or with a solution having a low ionic strength or high pH. In the conjugate, the substrate binding region is joined such as by covalent bonding to a non-protein chemical moiety such as a dye, chromophore, fluorescor, radionuclide or enzyme co-factor. By contacting the conjugate with a .beta.-1,4-glycan matrix, the substrate binding region binds to the matrix to immobilize the chemical moiety on the matrix. The conjugate or chemical moiety can be removed with a protease acting on a protease recognition sequence or with a solution having a low ionic strength or high pH.
公开/授权文献
- US6066784A Soybean cultivar J335752 公开/授权日:2000-05-23
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