发明授权
US6005092A Arabidopsis thaliana endo-1,4-.beta.-glucanase gene and promoter
失效
拟南芥内-4,4-β-葡聚糖酶基因和启动子
- 专利标题: Arabidopsis thaliana endo-1,4-.beta.-glucanase gene and promoter
- 专利标题(中): 拟南芥内-4,4-β-葡聚糖酶基因和启动子
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申请号: US6636申请日: 1998-01-13
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公开(公告)号: US6005092A公开(公告)日: 1999-12-21
- 发明人: Oded Shoseyov , Ziv Shani
- 申请人: Oded Shoseyov , Ziv Shani
- 申请人地址: ILX
- 专利权人: Yissum Research Development Company of the Hebrew University of Jerusalem
- 当前专利权人: Yissum Research Development Company of the Hebrew University of Jerusalem
- 当前专利权人地址: ILX
- 优先权: ILX121404 19970727
- 主分类号: A01H5/00
- IPC分类号: A01H5/00 ; A01H5/10 ; C07K14/33 ; C12N20060101 ; C12N5/04 ; C12N5/10 ; C12N5/14 ; C12N9/00 ; C12N9/36 ; C12N9/42 ; C12N15/09 ; C12N15/14 ; C12N15/29 ; C12N15/31 ; C12N15/63 ; C12N15/82 ; C12P19/04 ; C12N15/56
摘要:
The present invention discloses genetically engineered plants which display altered structure or morphology. The transgenic plants express a cell wall modulation transgene or gene construct that results in the altered structure or morphology. The altered structure or morphology can be associated with, for example, altered biomass, growth, yield, greater or less resistance to biodegradation, more or less digestible to ruminants, altered cellulose content, larger leaves/normal hypocotyls or smaller leaves/longer hypocotyls, etc. compared to a non-transgenic plant of the same species. The cell wall modulation transgene can be any cellulose binding domain, a cellulose binding protein, or a cell wall modifying protein or enzyme such as endoxyloglucan transferase, xyloglucan endo-transglycosylase, an expansin, cellulose synthase, or a novel isolated endo-1,4-.beta.-glucanase of Arabidopsis thaliana. The invention also discloses transgenic plants containing a gene construct comprising a promoter operably linked to the cell wall modulation protein or polypeptide gene and may further comprise a sequence encoding a secretion signal peptide. In particular, the invention discloses transgenic plants containing a gene construct comprising the cel1 promoter, operably linked to the cel1 signal peptide and any cellulose binding domain. Methods for modulating plant growth by transgenic expression of a cell wall modulating protein or polypeptide are also disclosed. The present invention also discloses a novel, isolated Arabidopsis thaliana endo-1,4-.beta.-glucanase gene (cel1), its promoter (cel1 promoter) and polypeptide (Cel1) and recombinant nucleic acid vectors containing the cel1 gene with or without a secretion signal peptide sequence and/or the cel1 promoter.
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