发明授权
- 专利标题： Method for isolation of highly pure von willebrand factor
- 专利标题（中）： 分离高纯度von willebrand因子的方法
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申请号： US898130申请日： 1997-07-22
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公开(公告)号： US6103693A公开(公告)日： 2000-08-15
- 发明人: Bernhard Fischer , Artur Mitterer , Friedrich Dorner , Hans-Peter Schwarz , Peter Turecek , Johann Eibl , Falko-Guenter Falkner , Uwe Schlokat , Wolfgang Mundt , Manfred Reiter , Renate Den-Bouwmeester
- 申请人： Bernhard Fischer , Artur Mitterer , Friedrich Dorner , Hans-Peter Schwarz , Peter Turecek , Johann Eibl , Falko-Guenter Falkner , Uwe Schlokat , Wolfgang Mundt , Manfred Reiter , Renate Den-Bouwmeester
- 申请人地址： ATX Vienna
- 专利权人： Immuno Aktiengesellschaft
- 当前专利权人： Immuno Aktiengesellschaft
- 当前专利权人地址： ATX Vienna
- 优先权： DEX4435485 19941004; WOXPCT/EP95/03892 19951002
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; A61K35/14 ; A61K38/00 ; A61K38/43 ; A61P7/04 ; C07K1/18 ; C07K1/22 ; C07K14/47 ; C07K14/755 ; C12P21/00 ; A61K38/17 ; C07K14/745
摘要：
The invention relates to a method for isolation of highly pure von Willebrand Factor in which recombinant von Willebrand Factor (rvWF) is chromatographically purified by anion exchange chromatography on an anion exchanger of the quaternary amino type in a buffer solution comprising buffer substances and optionally salt.The buffer solutions are preferably free of stabilizers, amino acids and other additives. According to this method, highly pure recombinant vWF can be obtained, which is free from blood plasma proteins, especially free from Factor VIII, and is physiologically active.Further, the invention relates to a pharmaceutical preparation that contains rvWF, which is comprised of multimers with a high structural integrity.