Methods of performing homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acid products thereof

发明授权

US06821759B1 Methods of performing homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acid products thereof 有权

标题翻译：在重组缺陷细胞中进行核酸的基于同源重组的修饰以及其修饰的核酸产物的使用的方法

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专利标题： Methods of performing homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acid products thereof
专利标题（中）： 在重组缺陷细胞中进行核酸的基于同源重组的修饰以及其修饰的核酸产物的使用的方法
申请号： US09619364

申请日： 2000-07-19
公开(公告)号： US06821759B1

公开(公告)日： 2004-11-23
发明人: Nathaniel Heintz , Peter Model , Xiangdong W. Yang , Shiaoching Gong
申请人： Nathaniel Heintz , Peter Model , Xiangdong W. Yang , Shiaoching Gong
主分类号： C12N1564
IPC分类号： C12N1564

Methods of performing homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acid products thereof

摘要：

A simple method for modifying genes in a recombination deficient host cell is disclosed. Such modifications include generating insertions, deletions, substitutions, and/or point mutations at any chosen site in the independent origin based cloning vector. The modified gene is contained in an independent origin based cloning vector that is used to introduce a modified heterologous gene into a cell. Such a modified vector may be used in the production of a germline transmitted transgenic animal, or in gene targeting protocols in eukaryotic cells. In particular, high throughput methodology is provided for generating the modified the independent origin based cloning vectors of the present invention.

摘要（中）：

公开了用于修饰重组缺陷宿主细胞中的基因的简单方法。这样的修饰包括在独立起始基克隆载体中的任何选择的位点产生插入，缺失，取代和/或点突变。修饰的基因包含在用于将修饰的异源基因引入细胞的独立的起始基克隆载体中。这种修饰的载体可用于生殖种系传播的转基因动物，或在真核细胞中的基因靶向方案中。特别地，提供了用于产生本发明的基于独立起源的克隆载体的高通量方法。

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