发明授权
- 专利标题: HCV variants
- 专利标题(中): HCV变体
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申请号: US09576989申请日: 2000-05-23
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公开(公告)号: US07049428B1公开(公告)日: 2006-05-23
- 发明人: Charles M. Rice, III , Keril J. Blight
- 申请人: Charles M. Rice, III , Keril J. Blight
- 申请人地址: US MO St. Louis
- 专利权人: Washington University
- 当前专利权人: Washington University
- 当前专利权人地址: US MO St. Louis
- 代理机构: Thompson Coburn LLP
- 主分类号: C07H21/00
- IPC分类号: C07H21/00 ; C07H21/04 ; C12N7/00 ; C12N7/02 ; C12N15/00
摘要:
HCV variants are described. The variants include polynucleotides comprising non-naturally occurring HCV sequences and HCV variants that have a transfection efficiency and ability to survive subpassage greater than HCV that have wild-type polyprotein coding regions. Expression vectors comprising the above polynucleotides and HCV variants are also described, as are the provision of cells and host cells comprising the expression vectors. Methods for identifying a cell line that is permissive for infection with HCV are also provided, as are vaccines comprising the above polynucleotides in a pharmaceutically acceptable carrier. Additionally, methods for inducing immunoprotection to HCV in a primate are described, as are methods for testing a compound for inhibiting HCV replication.