-
公开(公告)号：US07049428B1
公开(公告)日：2006-05-23
申请号：US09576989
申请日：2000-05-23
CPC分类号： C07K14/005 , C12N7/00 , C12N2770/24221 , C12N2770/24222
摘要： HCV variants are described. The variants include polynucleotides comprising non-naturally occurring HCV sequences and HCV variants that have a transfection efficiency and ability to survive subpassage greater than HCV that have wild-type polyprotein coding regions. Expression vectors comprising the above polynucleotides and HCV variants are also described, as are the provision of cells and host cells comprising the expression vectors. Methods for identifying a cell line that is permissive for infection with HCV are also provided, as are vaccines comprising the above polynucleotides in a pharmaceutically acceptable carrier. Additionally, methods for inducing immunoprotection to HCV in a primate are described, as are methods for testing a compound for inhibiting HCV replication.
摘要翻译： 描述了HCV变体。 变体包括包含非天然存在的HCV序列和HCV变体的多核苷酸，其具有转染效率和能够存活大于具有野生型多蛋白编码区的HCV的亚临床的能力。 还描述了包含上述多核苷酸和HCV变体的表达载体，以及提供包含表达载体的细胞和宿主细胞。 还提供了鉴定用于感染HCV的细胞系的方法，以及在药学上可接受的载体中包含上述多核苷酸的疫苗。 此外，还描述了在灵长类动物中诱导HCV的免疫保护的方法，以及用于测试抑制HCV复制的化合物的方法。
-
2.发明申请MICROPATTERNED CO-CULTURE SYSTEMS AS INFECTIOUS DISEASE ANALYSIS PLATFORMS 审中-公开微生物共培养系统作为感染性疾病分析平台公开(公告)号：US20110318730A1
公开(公告)日：2011-12-29
申请号：US13003214
申请日：2009-07-07
申请人： Charles M. Rice, III , Christopher Thomas Jones , Alexander Ploss , Sangeeta N. Bhatia , Salman Khetani
发明人： Charles M. Rice, III , Christopher Thomas Jones , Alexander Ploss , Sangeeta N. Bhatia , Salman Khetani
CPC分类号： G01N33/5067 , C12N2535/10 , C12Q1/18 , G01N33/5014
摘要： Cell cultures are provided that include a population of micropatterened hepatocytes and one or more non-parenchymal cell populations, where the hepatocytes are infected with a virus or parasite and include a reporter of virus or parasite infection. Methods of making and using the cell cultures are also provided.
摘要翻译： 提供细胞培养物，其包括微小细胞肝细胞群和一个或多个非实质细胞群体，其中肝细胞被病毒或寄生虫感染，并且包括病毒或寄生虫感染的报告物。 还提供了制备和使用细胞培养物的方法。
-
3.发明授权公开(公告)号：US07455969B2
公开(公告)日：2008-11-25
申请号：US10534571
申请日：2003-11-13
CPC分类号： C07K14/005 , C12N5/067 , C12N7/00 , C12N2770/24221 , C12N2770/24222 , C12N2770/24251
摘要： This invention relates generally to cells and cell lines that are permissive for hepatitis C virus (HCV) replication, and methods and materials for making and using them. The subject cell line referred to as Huh-7.5 has the A.T.C.C. designation number PTA-8561, having been deposited on Aug. 1, 2007.
摘要翻译： 本发明一般涉及对丙型肝炎病毒（HCV）复制是允许的细胞和细胞系，以及用于制备和使用它们的方法和材料。 称为Huh-7.5的主体细胞系具有A.T.C.C. 名称号码PTA-8561，已于2007年8月1日交存。
-
公开(公告)号：US07407758B2
公开(公告)日：2008-08-05
申请号：US11173792
申请日：2005-07-01
CPC分类号： C07K14/005 , C12N7/00 , C12N2770/24221 , C12N2770/24222
摘要： HCV variants are described. The variants include polynucleotides comprising non-naturally occurring HCV sequences and HCV variants that have a transfection efficiency and ability to survive subpassage greater than HCV that have wild-type polyprotein coding regions. Expression vectors comprising the above polynucleotides and HCV variants are also described, as are the provision of cells and host cells comprising the expression vectors. Methods for identifying a cell line that is permissive for infection with HCV are also provided, as are methods for identifying HCV variant polynucleotides with increased transfection efficiencies. Additionally, methods for identifying one or more HCV sequence mutations that provide drug-resistant HCV variants are disclosed.
摘要翻译： 描述了HCV变体。 变体包括包含非天然存在的HCV序列和HCV变体的多核苷酸，其具有转染效率和能够存活大于具有野生型多蛋白编码区的HCV的亚临床的能力。 还描述了包含上述多核苷酸和HCV变体的表达载体，以及提供包含表达载体的细胞和宿主细胞。 还提供了用于鉴定允许感染HCV的细胞系的方法，以及用于鉴定具有增加的转染效率的HCV变体多核苷酸的方法。 另外，公开了用于鉴定提供耐药HCV变体的一个或多个HCV序列突变的方法。
-
-
-
搜索结果
4 条记录 (耗时 0.026 秒)