发明授权
- 专利标题： Mesenchymal precursor cell
- 专利标题（中）： 间充质前体细胞
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申请号： US10030411申请日： 2000-07-07
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公开(公告)号： US07122178B1公开(公告)日： 2006-10-17
- 发明人: Paul Simmons , Andrew Zannettino , Stan Gronthos
- 申请人： Paul Simmons , Andrew Zannettino , Stan Gronthos
- 申请人地址： US NY New York
- 专利权人： Angioblast Systems, Incorporated
- 当前专利权人： Angioblast Systems, Incorporated
- 当前专利权人地址： US NY New York
- 代理机构： Cooper & Dunham LLP
- 代理商 John P. White, Esq.
- 优先权： AUPQ1477 19990707
- 国际申请： PCT/AU00/00822 WO 20000707
- 国际公布： WO01/04268 WO 20010118
- 主分类号： A01N63/00
- IPC分类号： A01N63/00 ; A61K35/26 ; C12N5/00 ; C12N5/08
摘要：
A method of enriching mesenchymal precursor sells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two color FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.