发明授权
- 专利标题: Process for discrimination of nucleic acid sequence variants
- 专利标题(中): 鉴别核酸序列变体的方法
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申请号: US11043099申请日: 2005-01-27
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公开(公告)号: US07141377B2公开(公告)日: 2006-11-28
- 发明人: David H. Gelfand , Pamela M. Holland , Randall K. Saiki , Robert M. Watson
- 申请人: David H. Gelfand , Pamela M. Holland , Randall K. Saiki , Robert M. Watson
- 申请人地址: US CA Alameda
- 专利权人: Roche Molecular Systems, Inc.
- 当前专利权人: Roche Molecular Systems, Inc.
- 当前专利权人地址: US CA Alameda
- 代理商 Charles M. Doyle
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12P19/34
摘要:
A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
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