A method of adjusting a sampling condition to generate a sampling clock in an analog to digital converter includes performing an analog to digital conversion on an analog input signal to thereby produce a digital sampled signal having a plurality of samples; calculating a difference value between two adjacent samples in the digital sampled signal; comparing the difference value with a threshold; adding the difference value into a sum of differences value if the difference value is greater than the threshold; and generating the sampling clock for the analog to digital converter according to the sum of differences value.