发明授权
- 专利标题: Methods of constructing camel antibody libraries
- 专利标题(中): 骆驼抗体文库的构建方法
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申请号: US10489477申请日: 2002-09-13
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公开(公告)号: US07371849B2公开(公告)日: 2008-05-13
- 发明人: Toshio Honda , Yasushi Akahori , Yoshikazu Kurosawa
- 申请人: Toshio Honda , Yasushi Akahori , Yoshikazu Kurosawa
- 申请人地址: JP Nagoya-shi
- 专利权人: Institute For Antibodies Co., Ltd.
- 当前专利权人: Institute For Antibodies Co., Ltd.
- 当前专利权人地址: JP Nagoya-shi
- 代理机构: Townsend and Townsend and Crew LLP
- 优先权: JP2001-277765 20010913
- 国际申请: PCT/JP02/09448 WO 20020913
- 国际公布: WO03/025020 WO 20030327
- 主分类号: C07H21/04
- IPC分类号: C07H21/04 ; C12P19/34 ; C12Q1/68
摘要:
The present invention provides camel antibody libraries that maintain in vivo diversity of camelid antibody variable region genes. The in vivo diversity of antibody variable region genes can be accomplished by, for example, mixing genes derived from a plurality of animals or modifying gene amplification conditions. Conventional methods yield only VHHs with limited repertoire diversity. However, the present invention provides libraries comprising genes encoding functional VHHs with sufficient repertoire size. According to the present invention, libraries that enable to freely obtain VHHs against arbitrary antigens are provided. VHHs have excellent solubility and stability, and show a reactivity that usually cannot be expected from tetrameric IgGs.
公开/授权文献
- US20050037421A1 Methods of constructing camel antibody libraries 公开/授权日:2005-02-17