公开(公告)号：US08101553B1

公开(公告)日：2012-01-24

申请号：US10204773

申请日：2001-02-22

申请人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Yoshitaka Iba ,  Kazuhiko Morino ,  Midori Shinohara ,  Motohide Takahashi ,  Yoshinobu Okuno ,  Kimiyasu Shiraki

发明人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Yoshitaka Iba ,  Kazuhiko Morino ,  Midori Shinohara ,  Motohide Takahashi ,  Yoshinobu Okuno ,  Kimiyasu Shiraki

IPC分类号： C40B50/06

CPC分类号： C12N15/10 ,  C07K16/00 ,  C07K2317/50 ,  C07K2317/56

摘要： An antibody library is prepared by selecting a light chain variable region capable of binding to the variable region of heavy chain to reproduce an active conformation and using the same. Because of being capable of maintaining the diversity of the heavy chain variable region at a high ratio in vitro, the antibody library of the present invention is expected as enabling the acquisition of antibodies with various binding activities.

摘要翻译： 通过选择能够结合重链可变区的轻链可变区以再现活性构象并使用它们来制备抗体文库。 由于能够在体外以高比例保持重链可变区的多样性，本发明的抗体文库预期能够获得具有各种结合活性的抗体。

公开(公告)号：US20050037421A1

公开(公告)日：2005-02-17

申请号：US10489477

申请日：2002-09-13

申请人： Toshio Honda ,  Yasushi Akahori ,  Yoshikazu Kurosawa

发明人： Toshio Honda ,  Yasushi Akahori ,  Yoshikazu Kurosawa

IPC分类号： C07K16/00 ,  C07K16/40 ,  C12Q1/68 ,  C07H21/04 ,  G01N33/53

CPC分类号： C07K16/40 ,  C07K16/00 ,  C07K16/005 ,  C07K2317/22 ,  C07K2317/52 ,  C07K2317/569

摘要： The present invention provides camel antibody libraries that maintain in vivo diversity of camelid antibody variable region genes. The in vivo diversity of antibody variable region genes can be accomplished by, for example, mixing genes derived from a plurality of animals or modifying gene amplification conditions. Conventional methods yield only VHHs with limited repertoire diversity. However, the present invention provides libraries comprising genes encoding functional VHHs with sufficient repertoire size. According to the present invention, libraries that enable to freely obtain VHHs against arbitrary antigens are provided. VHHs have excellent solubility and stability, and show a reactivity that usually cannot be expected from tetrameric IgGs.

摘要翻译： 本发明提供了保持骆驼抗体可变区基因的体内多样性的骆驼抗体文库。 抗体可变区基因的体内多样性可以通过例如混合衍生自多种动物的基因或修饰基因扩增条件来实现。 常规方法仅产生具有有限谱库多样性的VHH。 然而，本发明提供了包含编码具有足够谱库大小的功能性VHH的基因的文库。 根据本发明，提供能够自由获得针对任意抗原的VHH的文库。 VHH具有优异的溶解度和稳定性，并显示通常不能从四聚体IgGs预期的反应性。

公开(公告)号：US07371849B2

公开(公告)日：2008-05-13

申请号：US10489477

申请日：2002-09-13

申请人： Toshio Honda ,  Yasushi Akahori ,  Yoshikazu Kurosawa

发明人： Toshio Honda ,  Yasushi Akahori ,  Yoshikazu Kurosawa

IPC分类号： C07H21/04 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C07K16/40 ,  C07K16/00 ,  C07K16/005 ,  C07K2317/22 ,  C07K2317/52 ,  C07K2317/569

摘要： The present invention provides camel antibody libraries that maintain in vivo diversity of camelid antibody variable region genes. The in vivo diversity of antibody variable region genes can be accomplished by, for example, mixing genes derived from a plurality of animals or modifying gene amplification conditions. Conventional methods yield only VHHs with limited repertoire diversity. However, the present invention provides libraries comprising genes encoding functional VHHs with sufficient repertoire size. According to the present invention, libraries that enable to freely obtain VHHs against arbitrary antigens are provided. VHHs have excellent solubility and stability, and show a reactivity that usually cannot be expected from tetrameric IgGs.

摘要翻译： 本发明提供了保持骆驼抗体可变区基因的体内多样性的骆驼抗体文库。 抗体可变区基因的体内多样性可以通过例如混合衍生自多种动物的基因或修饰基因扩增条件来实现。 常规方法仅产生具有有限谱库多样性的VHH。 然而，本发明提供了包含编码具有足够谱库大小的功能性VHH的基因的文库。 根据本发明，提供能够自由获得针对任意抗原的VHH的文库。 VHH具有优异的溶解度和稳定性，并显示通常不能从四聚体IgGs预期的反应性。

公开(公告)号：US20120046451A1

公开(公告)日：2012-02-23

申请号：US13238096

申请日：2011-09-21

申请人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Nobuhiro Takahashi ,  Atsushi Sugioka ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Kazuhiro Suzuki ,  Miwa Morita ,  Gene Kurosawa ,  Sari Fujiyama ,  Susumu Tsutsumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Yoshitaka Iba ,  Mariko Sumitomo ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Morishita

发明人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Nobuhiro Takahashi ,  Atsushi Sugioka ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Kazuhiro Suzuki ,  Miwa Morita ,  Gene Kurosawa ,  Sari Fujiyama ,  Susumu Tsutsumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Yoshitaka Iba ,  Mariko Sumitomo ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Morishita

IPC分类号： C07K16/42

CPC分类号： C07K16/303 ,  A61K2039/505 ,  C07K16/2803 ,  C07K2317/52 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2319/00 ,  G01N33/57426 ,  G01N33/57438

摘要： It is intended to clarify a molecule which is available as a target in treating or diagnosing cancer and utilize the molecule in the medical field or the research field. By treating IgSF4, which has been identified as a molecule specifically expressed in lung cancer cells, with an antibody, and ADCC activity is exerted. Based on this finding, an anti-IgSF4 antibody is provided as a means efficacious in treating cancer, etc.

摘要翻译： 旨在澄清可用作治疗或诊断癌症并在医学领域或研究领域中利用分子的靶标的分子。 通过用抗体处理被鉴定为特异性表达于肺癌细胞中的分子的IgSF4，并施加ADCC活性。 基于该发现，提供了抗IgSF4抗体作为治疗癌症等的手段。

公开(公告)号：US08048992B2

公开(公告)日：2011-11-01

申请号：US11885245

申请日：2006-02-22

申请人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Nobuhiro Takahashi ,  Atsushi Sugioka ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Kazuhiro Suzuki ,  Miwa Morita ,  Gene Kurosawa ,  Sari Fujiyama ,  Susumu Tsustumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Yoshitaka Iba ,  Mariko Sumitomo ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Morishita

发明人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Nobuhiro Takahashi ,  Atsushi Sugioka ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Kazuhiro Suzuki ,  Miwa Morita ,  Gene Kurosawa ,  Sari Fujiyama ,  Susumu Tsustumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Yoshitaka Iba ,  Mariko Sumitomo ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Morishita

IPC分类号： C07K16/00

CPC分类号： C07K16/303 ,  A61K2039/505 ,  C07K16/2803 ,  C07K2317/52 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2319/00 ,  G01N33/57426 ,  G01N33/57438

摘要： It is intended to clarify a molecule which is available as a target in treating or diagnosing cancer and utilize the molecule in the medical field or the research field. By treating IgSF4, which has been identified as a molecule specifically expressed in lung cancer cells, with an antibody, and ADCC activity is exerted. Based on this finding, an anti-IgSF4 antibody is provided as a means efficacious in treating cancer, etc.

摘要翻译： 旨在澄清可用作治疗或诊断癌症并在医学领域或研究领域中利用分子的靶标的分子。 通过用抗体处理被鉴定为特异性表达于肺癌细胞中的分子的IgSF4，并施加ADCC活性。 基于该发现，提供了抗IgSF4抗体作为治疗癌症等的手段。

公开(公告)号：US20090203538A1

公开(公告)日：2009-08-13

申请号：US12318829

申请日：2009-01-09

申请人： Atsushi Sugioka ,  Mototaka Sugiura ,  Yasushi Akahori ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Miwa Morita ,  Gene Kurosawa ,  Mariko Sumitomo ,  Susumu Tsutsumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Noriko Satou ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Suzuki ,  Yoshikazu Kurosawa ,  Miho Tanaka ,  Mamoru Shiraishi

发明人： Atsushi Sugioka ,  Mototaka Sugiura ,  Yasushi Akahori ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Miwa Morita ,  Gene Kurosawa ,  Mariko Sumitomo ,  Susumu Tsutsumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Noriko Satou ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Suzuki ,  Yoshikazu Kurosawa ,  Miho Tanaka ,  Mamoru Shiraishi

IPC分类号： C40B30/04 ,  G01N33/567 ,  G01N33/574 ,  C07K16/00 ,  C40B40/10 ,  C40B60/10

CPC分类号： C07K16/30 ,  C07K16/005 ,  C07K16/28 ,  C07K16/2803 ,  C07K16/2863 ,  C07K16/2896 ,  C07K16/303 ,  C07K16/32 ,  C07K16/40 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/732 ,  C12N15/1138 ,  C12N2310/14 ,  G01N33/57492 ,  G01N33/6854

摘要： It is intended to provide a method whereby a plural number of antibodies against cell surface antigens are quickly classified and to provide a method whereby antigens of the thus classified antibodies are quickly identified. Further, it is intended to provide a method of promoting the utilization of the useful data obtained by the above methods. Furthermore, it is intended to provide an antibody which is effective in treating or diagnosing cancer. Namely, a method of classifying antibodies which comprises: (1) the step of preparing a plural number of antibodies respectively recognizing cell surface antigens; (2) the step of bringing each of these antibodies into contact with a cell of the same species; (3) the step of analyzing each of the cells having been treated in the step (2) by flow cytometry and thus obtaining data indicating the reactivity of each antibody with its cell surface antigen; and (4) the step of comparing the thus obtained data and classifying the individual antibodies depending on the similarity. A method of identifying antigens which further comprises: (5) the step of selecting one to several antibodies from each antibody group formed in the step (4) and identifying antigens thereof; and (6) on the assumption that antigens of the antibodies belonging to a single antibody group are the same or highly related to one another, making relations between the antigens having been identified in the step (5) and the antibody groups to thereby identify the antigens. An antibody against HER1, an antibody against HER2, an antibody against CD46, an antibody against ITGA3, an antibody against ICAM1, an antibody against ALCAM, an antibody against CD147, an antibody against C1qR, an antibody against CD44, an antibody against CD73, an antibody against EpCAM and an antibody against HGFR, each obtained by using the above methods.

公开(公告)号：US20090053243A1

公开(公告)日：2009-02-26

申请号：US11885245

申请日：2006-02-22

申请人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Nobuhiro Takahashi ,  Atsushi Sugioka ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Kazuhiro Suzuki ,  Miwa Morita ,  Gene Kurosawa ,  Sari Fujiyama ,  Susumu Tsustumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Yoshitaka Iba ,  Mariko Sumitomo ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Morishita

发明人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Nobuhiro Takahashi ,  Atsushi Sugioka ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Kazuhiro Suzuki ,  Miwa Morita ,  Gene Kurosawa ,  Sari Fujiyama ,  Susumu Tsustumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Yoshitaka Iba ,  Mariko Sumitomo ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Morishita

IPC分类号： A61K39/395 ,  C07K16/18 ,  C12N15/11 ,  C12N15/00 ,  A61P31/00 ,  C12N5/06 ,  G01N33/574

CPC分类号： C07K16/303 ,  A61K2039/505 ,  C07K16/2803 ,  C07K2317/52 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2319/00 ,  G01N33/57426 ,  G01N33/57438

摘要： It is intended to clarify a molecule which is available as a target in treating or diagnosing cancer and utilize the molecule in the medical field or the research field. By treating IgSF4, which has been identified as a molecule specifically expressed in lung cancer cells, with an antibody, and ADCC activity is exerted. Based on this finding, an anti-IgSF4 antibody is provided as a means efficacious in treating cancer, etc.

摘要翻译： 旨在澄清可用作治疗或诊断癌症并在医学领域或研究领域中利用分子的靶标的分子。 通过用抗体处理被鉴定为特异性表达于肺癌细胞中的分子的IgSF4，并施加ADCC活性。 基于该发现，提供了抗IgSF4抗体作为治疗癌症等的手段。

公开(公告)号：US5984647A

公开(公告)日：1999-11-16

申请号：US52982

申请日：1998-04-01

申请人： Fumiyuki Miyamoto ,  Yoshinori Shirafuji ,  Shinobu Ogasawara ,  Yasushi Akahori ,  Hitoshi Tokuyama ,  Yukio Hidaka ,  Hirofumi Fujioka

发明人： Fumiyuki Miyamoto ,  Yoshinori Shirafuji ,  Shinobu Ogasawara ,  Yasushi Akahori ,  Hitoshi Tokuyama ,  Yukio Hidaka ,  Hirofumi Fujioka

IPC分类号： F04C29/00 ,  H01B3/44 ,  H02K3/30 ,  H02K15/12 ,  F04B17/03 ,  F25B31/02

CPC分类号： H01B3/44 ,  H02K3/30 ,  Y10T29/49009

摘要： A process for producing a hermetic electric compressor for a refrigerating or air-conditioning apparatus using a refrigerant having a hydrofluorocarbon base which comprises a motor unit, a compressing unit driven by the motor unit and a sealed housing enclosing the motor unit and the compressing unit and stores a refrigeration oil on the bottom of the sealed housing, wherein a solventless varnish comprising a thermosetting resin having at least two (meth)acryloyl groups in one molecule, a low-viscosity vinyl or 1-alkylvinyl monomer having an ether or ester linkage as a reactive diluent and an organic peroxide as a reaction initiator is used as an insulating varnish to insulate and fix the magnet wires of the stator of the motor unit, and the solventless varnish is cured by heating at a temperature of from 153 to 180.degree. C. for from 0.5 to 3.5 hours.

摘要翻译： 一种使用具有氢氟烃基底的制冷剂的制冷或空气调节装置的密闭式电动压缩机的制造方法，该制冷剂或空气调节装置包括马达单元，由马达单元驱动的压缩单元和封闭马达单元和压缩单元的密封壳体， 在所述密封壳体的底部存储冷冻机油，其中，包含在一分子中具有至少两个（甲基）丙烯酰基的热固性树脂的无溶剂清漆，具有醚或酯键的低粘度乙烯基或1-烷基乙烯基单体作为 使用反应性稀释剂和作为反应引发剂的有机过氧化物作为绝缘漆，以使马达单元的定子的电线绝缘和固定，并且将无溶剂清漆通过在153-180℃的温度下加热而固化 0.5至3.5小时。

公开(公告)号：US5531080A

公开(公告)日：1996-07-02

申请号：US273848

申请日：1994-07-12

申请人： Takuho Hirahara ,  Susumu Kawaguchi ,  Tatsuaki Shimizu ,  Katsuyuki Kawasaki ,  Noboru Masuda ,  Shinobu Ogasawara ,  Hiroshige Konishi ,  Hitoshi Maruyama ,  Yoshihiro Sumida ,  Akemi Ueyama ,  Satoru Toyama ,  Sou Suzuki ,  Yasushi Akahori

发明人： Takuho Hirahara ,  Susumu Kawaguchi ,  Tatsuaki Shimizu ,  Katsuyuki Kawasaki ,  Noboru Masuda ,  Shinobu Ogasawara ,  Hiroshige Konishi ,  Hitoshi Maruyama ,  Yoshihiro Sumida ,  Akemi Ueyama ,  Satoru Toyama ,  Sou Suzuki ,  Yasushi Akahori

IPC分类号： C09K5/04 ,  C10M171/00 ,  F04C23/00 ,  F25B9/00 ,  F25B31/00 ,  F25B43/00 ,  F25B43/02

CPC分类号： F04C23/008 ,  C09K5/045 ,  C10M171/008 ,  F25B31/002 ,  F25B43/006 ,  F25B9/006 ,  C09K2205/22 ,  C09K2205/24 ,  C09K2205/32 ,  C09K2205/34 ,  C10M2201/066 ,  C10M2203/06 ,  C10M2203/10 ,  C10M2203/102 ,  C10M2203/104 ,  C10M2203/106 ,  C10M2203/108 ,  C10M2205/00 ,  C10M2205/02 ,  C10M2207/023 ,  C10M2207/024 ,  C10M2207/026 ,  C10M2207/04 ,  C10M2207/042 ,  C10M2207/121 ,  C10M2207/122 ,  C10M2207/125 ,  C10M2207/129 ,  C10M2207/402 ,  C10M2209/00 ,  C10M2209/02 ,  C10M2209/10 ,  C10M2211/02 ,  C10M2211/022 ,  C10M2211/042 ,  C10M2211/044 ,  C10M2211/06 ,  C10M2211/08 ,  C10M2215/02 ,  C10M2215/04 ,  C10M2215/064 ,  C10M2215/065 ,  C10M2215/26 ,  C10M2219/00 ,  C10M2219/068 ,  C10M2219/082 ,  C10M2219/086 ,  C10M2219/087 ,  C10M2219/089 ,  C10M2219/10 ,  C10M2219/102 ,  C10M2219/104 ,  C10M2219/106 ,  C10M2219/108 ,  C10M2221/02 ,  C10M2223/04 ,  C10M2223/042 ,  C10M2223/045 ,  C10M2223/047 ,  C10M2227/00 ,  C10M2229/041 ,  C10N2240/00 ,  C10N2240/22 ,  C10N2240/30 ,  C10N2240/50 ,  C10N2240/52 ,  C10N2240/54 ,  C10N2240/56 ,  C10N2240/58 ,  C10N2240/60 ,  C10N2240/66 ,  F25B2400/12

摘要： A refrigerant circulating system that uses a highly polar refrigerant, as typified by a hydrofluorocarbon-based one, and a refrigerating oil that has slight solubility with a liquid refrigerant on the condensation side. Also, a refrigerant circulating cycle that uses a highly polar refrigerant, as typified by a hydrofluorocarbon-based one, and a refrigerating oil that has a slight solubility with a liquid refrigerant and which has a greater specific gravity than the liquid refrigerant. Thereby, it provides a high electrical insulation and moisture resistance, good oil return to the compressor, and high reliability.

摘要翻译： 使用以氢氟烃类为代表的高极性制冷剂的制冷剂循环系统和在冷凝侧与液体制冷剂具有轻微溶解性的冷冻机油。 此外，使用以氢氟烃类为代表的高极性制冷剂的制冷剂循环循环和与液体制冷剂具有轻微溶解性并且比液体制冷剂具有更大比重的冷冻机油。 因此，它提供了高电气绝缘和防潮性，良好的油返回到压缩机，并具有高可靠性。

公开(公告)号：US06221274B1

公开(公告)日：2001-04-24

申请号：US09233199

申请日：1999-01-20

申请人： Yasushi Akahori ,  Noboru Masuda ,  Takeshi Izawa ,  Masayoshi Muraki ,  Kazuo Tagawa ,  Tomohiro Magome

发明人： Yasushi Akahori ,  Noboru Masuda ,  Takeshi Izawa ,  Masayoshi Muraki ,  Kazuo Tagawa ,  Tomohiro Magome

IPC分类号： C10M12918

CPC分类号： C10M169/04 ,  C09K5/045 ,  C10M171/008 ,  C10M2207/042 ,  C10M2207/281 ,  C10M2207/282 ,  C10M2207/283 ,  C10M2207/286 ,  C10M2211/022 ,  C10M2211/06 ,  C10M2223/04 ,  C10M2223/041 ,  C10M2223/042 ,  C10M2223/047 ,  C10N2240/00 ,  C10N2240/22 ,  C10N2240/30 ,  C10N2240/50 ,  C10N2240/52 ,  C10N2240/54 ,  C10N2240/56 ,  C10N2240/58 ,  C10N2240/60 ,  C10N2240/66

摘要： A lubricant composition for a refrigerating machine employing a HFC type refrigerant containing at least one selected from the group consisting of HFC-32, HFC-125 and HFC-134a, which comprises a base oil of an ester formed from a dihydric or higher polyol as an alcohol and a monobasic aliphatic acid, alone or as a mixture thereof; and additives of, based on the total amount, 1) not less than 0.01 vol % and less than 1.0 vol % of a phosphate, 2) from 0.01 to 1.0 vol % of at least one of an alkylphosphorothionate and an arylphosphorothionate and 3) from 0.01 to 1.0 vol % of an epoxy compound.

摘要翻译： 一种使用含有选自HFC-32，HFC-125和HFC-134a中的至少一种的HFC型制冷剂的制冷机的润滑剂组合物，其包含由二羟基或更高级多元醇形成的酯的基础油作为 醇和一元脂肪酸，单独或作为其混合物; 和基于总量的添加剂，1）不小于0.01vol％且小于1.0vol％的磷酸盐，2）0.01至1.0vol％的至少一种烷基硫代硫酸盐和芳基硫代硫酸盐，以及3）从 0.01〜1.0体积％的环氧化合物。