发明授权
- 专利标题: Tandem isotachophoresis/zone electrophoresis method and system
- 专利标题(中): 串联等电泳/区域电泳方法和系统
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申请号: US10676857申请日: 2003-09-30
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公开(公告)号: US07494577B2公开(公告)日: 2009-02-24
- 发明人: Stephen J. Williams , Hong Dong Tan , Hung Pin Kao , Wyatt N. Vreeland
- 申请人: Stephen J. Williams , Hong Dong Tan , Hung Pin Kao , Wyatt N. Vreeland
- 申请人地址: US CA South San Francisco
- 专利权人: Monogram Biosciences, Inc.
- 当前专利权人: Monogram Biosciences, Inc.
- 当前专利权人地址: US CA South San Francisco
- 代理机构: Perkins Coie LLP
- 主分类号: G01N27/447
- IPC分类号: G01N27/447
摘要:
A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte. With the application of a voltage potential across the microchannel, charged components in the sample stack by isotachophoresis, and electrolytic hydroxyl or hydrogen ions formed by electrolysis at the upstream-end electrode migrate into the trailing-edge ion buffer, titrating the titratable species therein, where the concentration of the titratable species in the trailing-edge electrolyte is selected , in relation to the lengths of the upstream channel region and sample-loading volume, to permit the sample to stack into a relatively small sample volume before electrolytic-ion migration from the upstream electrode into and through the sample-volume region is effective to overtake the charge sample components. With continued application of an electric potential across the channel ends, charged sample components in the stacked sample volume separated by zone electrophoresis.