公开(公告)号：US07494577B2

公开(公告)日：2009-02-24

申请号：US10676857

申请日：2003-09-30

申请人： Stephen J. Williams ,  Hong Dong Tan ,  Hung Pin Kao ,  Wyatt N. Vreeland

发明人： Stephen J. Williams ,  Hong Dong Tan ,  Hung Pin Kao ,  Wyatt N. Vreeland

IPC分类号： G01N27/447

CPC分类号： B01L3/5027 ,  B01L3/502753 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/049 ,  C07K1/26 ,  C07K1/28 ,  G01N27/44773

摘要： A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte. With the application of a voltage potential across the microchannel, charged components in the sample stack by isotachophoresis, and electrolytic hydroxyl or hydrogen ions formed by electrolysis at the upstream-end electrode migrate into the trailing-edge ion buffer, titrating the titratable species therein, where the concentration of the titratable species in the trailing-edge electrolyte is selected , in relation to the lengths of the upstream channel region and sample-loading volume, to permit the sample to stack into a relatively small sample volume before electrolytic-ion migration from the upstream electrode into and through the sample-volume region is effective to overtake the charge sample components. With continued application of an electric potential across the channel ends, charged sample components in the stacked sample volume separated by zone electrophoresis.

摘要翻译： 公开了一种分离具有给定负电荷并包含在样品中的组分的方法。 在一个实施方案中，该方法包括将具有样品的微通道加载到放置在具有选定浓度的可滴定物质的后缘电解质和前沿电解质之间。 通过在微通道上施加电压电位，通过等速电泳施加样品叠层中的带电组分，并且通过在上游端电极处电解形成的电解羟基或氢离子迁移到后缘离子缓冲液中，滴定其中的可滴定物质， 其中选择后缘电解质中的可滴定物质的浓度，相对于上游通道区域的长度和样品加载体积，以允许样品在电离离子迁移之前堆积成相对小的样品体积 进入和通过样品体积区域的上游电极有效地超过电荷样品组分。 通过在通道末端继续施加电位，通过区域电泳分离的堆叠样品体积中的带电样品组分。

公开(公告)号：US06685813B2

公开(公告)日：2004-02-03

申请号：US09933993

申请日：2001-08-20

申请人： Stephen J. Williams ,  Hong Dong Tan ,  Hung Pin Kao ,  Wyatt N. Vreeland

发明人： Stephen J. Williams ,  Hong Dong Tan ,  Hung Pin Kao ,  Wyatt N. Vreeland

IPC分类号： B01D5902

CPC分类号： B01L3/5027 ,  B01L3/502753 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/049 ,  C07K1/26 ,  C07K1/28 ,  G01N27/44773

摘要： A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte. With the application of a voltage potential across the microchannel, charged components in the sample stack by isotachophoresis, and electrolytic hydroxyl or hydrogen ions formed by electrolysis at the upstream-end electrode migrate into the trailing-edge ion buffer, titrating the titratable species therein, where the concentration of the titratable species in the trailing-edge electrolyte is selected, in relation to the lengths of the upstream channel region and sample-loading volume, to permit the sample to stack into a relatively small sample volume before electrolytic-ion migration from the upstream electrode into and through the sample-volume region is effective to overtake the charged sample components. With continued application of an electric potential across the channel ends, charged sample components in the stacked sample volume separate by zone electrophoresis.

摘要翻译： 公开了一种分离具有给定负电荷并包含在样品中的组分的方法。 在一个实施方案中，该方法包括将具有样品的微通道加载到放置在具有选定浓度的可滴定物质的后缘电解质和前沿电解质之间。 通过在微通道上施加电压电位，通过等速电泳施加样品叠层中的带电组分，并且通过在上游端电极处电解形成的电解羟基或氢离子迁移到后缘离子缓冲液中，滴定其中的可滴定物质， 其中选择后缘电解质中的可滴定物质的浓度，相对于上游通道区域的长度和样品加载体积，以允许样品在电离离子迁移之前堆积成相对小的样品体积 进入和通过样品体积区域的上游电极有效地超过带电的样品组分。 通过在通道末端继续施加电位，通过区域电泳分离堆叠样品体积中的带电样品组分。

公开(公告)号：US06818113B2

公开(公告)日：2004-11-16

申请号：US09780638

申请日：2001-02-10

申请人： Stephen J. Williams ,  Hong Dong Tan ,  Hung Pin Kao ,  Wyatt N. Vreeland

发明人： Stephen J. Williams ,  Hong Dong Tan ,  Hung Pin Kao ,  Wyatt N. Vreeland

IPC分类号： B01D5702

CPC分类号： C07K1/28 ,  B01L3/5027 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/049 ,  G01N27/44743 ,  G01N27/44747 ,  G01N27/44773 ,  G01N27/44791

摘要： A method and device for injecting a liquid sample into an electrolyte channel in a microfluidics device is disclosed. The device has a channel network that includes an electrolyte channel having upstream and downstream channel portions and first, second, and third side channels that intersect the electrolyte channel between the two channel portions at first, second, and third ports, respectively. In the method, a sample is moved electrokinetically into the electrolyte channel, to form a defined sample volume therein. By simultaneously controlling the voltage applied to the three side channels, and at least one of the upstream and downstream channel end portions, the sample volume element can be shaped to have a desired leading- and trailing-edge shape and/or distribution of sample components within the volume elements.

摘要翻译： 公开了一种用于将液体样品注入微流体装置中的电解质通道的方法和装置。 该装置具有通道网络，其包括具有上游和下游通道部分的电解质通道以及分别在第一，第二和第三端口处的两个通道部分之间与电解质通道相交的第一，第二和第三侧通道。 在该方法中，将样品电动移动到电解质通道中，以在其中形成限定的样品体积。 通过同时控制施加到三个侧通道的电压以及上游和下游通道端部中的至少一个，样品体积元件可以被成形为具有期望的前沿和后沿形状和/或样品组分的分布 在音量元素内。