Microfluidic device with sample injector and method of using
    1.
    发明授权
    Microfluidic device with sample injector and method of using 有权
    具有样品注射器的微流体装置和使用方法

    公开(公告)号:US06818113B2

    公开(公告)日:2004-11-16

    申请号:US09780638

    申请日:2001-02-10

    IPC分类号: B01D5702

    摘要: A method and device for injecting a liquid sample into an electrolyte channel in a microfluidics device is disclosed. The device has a channel network that includes an electrolyte channel having upstream and downstream channel portions and first, second, and third side channels that intersect the electrolyte channel between the two channel portions at first, second, and third ports, respectively. In the method, a sample is moved electrokinetically into the electrolyte channel, to form a defined sample volume therein. By simultaneously controlling the voltage applied to the three side channels, and at least one of the upstream and downstream channel end portions, the sample volume element can be shaped to have a desired leading- and trailing-edge shape and/or distribution of sample components within the volume elements.

    摘要翻译: 公开了一种用于将液体样品注入微流体装置中的电解质通道的方法和装置。 该装置具有通道网络,其包括具有上游和下游通道部分的电解质通道以及分别在第一,第二和第三端口处的两个通道部分之间与电解质通道相交的第一,第二和第三侧通道。 在该方法中,将样品电动移动到电解质通道中,以在其中形成限定的样品体积。 通过同时控制施加到三个侧通道的电压以及上游和下游通道端部中的至少一个,样品体积元件可以被成形为具有期望的前沿和后沿形状和/或样品组分的分布 在音量元素内。

    Tandem isotachophoresis/zone electrophoresis method and system
    2.
    发明授权
    Tandem isotachophoresis/zone electrophoresis method and system 有权
    串联等电泳/区域电泳方法和系统

    公开(公告)号:US07494577B2

    公开(公告)日:2009-02-24

    申请号:US10676857

    申请日:2003-09-30

    IPC分类号: G01N27/447

    摘要: A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte. With the application of a voltage potential across the microchannel, charged components in the sample stack by isotachophoresis, and electrolytic hydroxyl or hydrogen ions formed by electrolysis at the upstream-end electrode migrate into the trailing-edge ion buffer, titrating the titratable species therein, where the concentration of the titratable species in the trailing-edge electrolyte is selected , in relation to the lengths of the upstream channel region and sample-loading volume, to permit the sample to stack into a relatively small sample volume before electrolytic-ion migration from the upstream electrode into and through the sample-volume region is effective to overtake the charge sample components. With continued application of an electric potential across the channel ends, charged sample components in the stacked sample volume separated by zone electrophoresis.

    摘要翻译: 公开了一种分离具有给定负电荷并包含在样品中的组分的方法。 在一个实施方案中,该方法包括将具有样品的微通道加载到放置在具有选定浓度的可滴定物质的后缘电解质和前沿电解质之间。 通过在微通道上施加电压电位,通过等速电泳施加样品叠层中的带电组分,并且通过在上游端电极处电解形成的电解羟基或氢离子迁移到后缘离子缓冲液中,滴定其中的可滴定物质, 其中选择后缘电解质中的可滴定物质的浓度,相对于上游通道区域的长度和样品加载体积,以允许样品在电离离子迁移之前堆积成相对小的样品体积 进入和通过样品体积区域的上游电极有效地超过电荷样品组分。 通过在通道末端继续施加电位,通过区域电泳分离的堆叠样品体积中的带电样品组分。

    Tandem isotachophoresis/zone electrophoresis method and system
    3.
    发明授权
    Tandem isotachophoresis/zone electrophoresis method and system 有权
    串联等电泳/区域电泳方法和系统

    公开(公告)号:US06685813B2

    公开(公告)日:2004-02-03

    申请号:US09933993

    申请日:2001-08-20

    IPC分类号: B01D5902

    摘要: A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte. With the application of a voltage potential across the microchannel, charged components in the sample stack by isotachophoresis, and electrolytic hydroxyl or hydrogen ions formed by electrolysis at the upstream-end electrode migrate into the trailing-edge ion buffer, titrating the titratable species therein, where the concentration of the titratable species in the trailing-edge electrolyte is selected, in relation to the lengths of the upstream channel region and sample-loading volume, to permit the sample to stack into a relatively small sample volume before electrolytic-ion migration from the upstream electrode into and through the sample-volume region is effective to overtake the charged sample components. With continued application of an electric potential across the channel ends, charged sample components in the stacked sample volume separate by zone electrophoresis.

    摘要翻译: 公开了一种分离具有给定负电荷并包含在样品中的组分的方法。 在一个实施方案中,该方法包括将具有样品的微通道加载到放置在具有选定浓度的可滴定物质的后缘电解质和前沿电解质之间。 通过在微通道上施加电压电位,通过等速电泳施加样品叠层中的带电组分,并且通过在上游端电极处电解形成的电解羟基或氢离子迁移到后缘离子缓冲液中,滴定其中的可滴定物质, 其中选择后缘电解质中的可滴定物质的浓度,相对于上游通道区域的长度和样品加载体积,以允许样品在电离离子迁移之前堆积成相对小的样品体积 进入和通过样品体积区域的上游电极有效地超过带电的样品组分。 通过在通道末端继续施加电位,通过区域电泳分离堆叠样品体积中的带电样品组分。

    Chiral temperature gradient focusing
    5.
    发明授权
    Chiral temperature gradient focusing 失效
    手性温度梯度聚焦

    公开(公告)号:US07572357B2

    公开(公告)日:2009-08-11

    申请号:US11039955

    申请日:2005-01-24

    IPC分类号: G01N27/447

    CPC分类号: G01N27/44795 G01N27/44734

    摘要: A method and device are provided for concentrating and separating materials in fluids within a fluidic device having a fluid conduit such as a channel or capillary. The concentration is achieved by balancing the electrophoretic velocity of a material against the bulk flow of fluid in the presence of a temperature gradient. An additive is added to the fluid which interacts with the material and which modifies the normal electrophoretic mobility of the material. Using an appropriate fluid, the temperature gradient can generate a corresponding gradient in the electrophoretic velocity so that the electrophoretic and bulk velocities sum to zero at a unique position along the conduit and the material will be focused at that position. The method and device may be adapted for use with a variety of materials including fluorescent dyes, amino acids, proteins, DNA and to concentrate a dilute material.

    摘要翻译: 提供了一种方法和装置,用于在具有诸如通道或毛细管的流体导管的流体装置内的流体中浓缩和分离材料。 通过在存在温度梯度的情况下平衡材料的电泳速度与流体的体积流动来实现浓度。 将添加剂添加到与材料相互作用并且改变材料的正常电泳迁移率的流体中。 使用适当的流体,温度梯度可以产生相应的电泳速度梯度,使得电泳和体积速度在沿着导管的独特位置处总和为零,并且材料将聚焦在该位置。 该方法和装置可适用于各种材料,包括荧光染料,氨基酸,蛋白质,DNA和浓缩稀释材料。

    Micellar gradient focusing
    7.
    发明授权
    Micellar gradient focusing 有权
    胶束梯度聚焦

    公开(公告)号:US07718046B2

    公开(公告)日:2010-05-18

    申请号:US10864485

    申请日:2004-06-10

    IPC分类号: B01D57/02

    摘要: A method and device are provided for affinity gradient focusing for directing at least one analyte in a solution containing a pseudostationary phase and located in a channel such as a capillary or a microchannel. The method includes establishing a steady-state spatial gradient in a retention factor of the pseudostationary phase for the at least one analyte. The analyte is caused to be moved within the channel whereby the concentration of the at least one analyte changes at one or more positions along the gradient. The pseudostationary phase is charged and the analyte is either neutral or charged or alternatively, the pseudostationary phase is neutral and the analyte is charged. The device may include a fluid channel, a pseudostationary phase having a retention factor gradient, an electrical current source and a pump system for establishing the bulk flow in the solution in the channel.

    摘要翻译: 提供了一种用于亲和梯度聚焦的方法和装置,用于将至少一种分析物引导到含有假阶段并且位于诸如毛细管或微通道的通道中的溶液中。 该方法包括在至少一种分析物的假定相的保留因子中建立稳态空间梯度。 导致分析物在通道内移动,由此至少一种分析物的浓度在沿梯度的一个或多个位置处变化。 假定相被充电,分析物是中性的或带电的,或者替代地,假定相是中性的并且分析物被充电。 该装置可以包括流体通道,具有保持因子梯度的假定相,电流源和用于在通道中的溶液中建立大量流的泵系统。