发明授权
- 专利标题: Production of antimicrobial proteins in fusion proteins
- 专利标题(中): 融合蛋白中抗微生物蛋白的生产
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申请号: US10544414申请日: 2004-02-06
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公开(公告)号: US07785828B1公开(公告)日: 2010-08-31
- 发明人: Gusui Wu , Wessel Lageweg , Maarten H. Stuiver , Lu Liu , Wei Wei , Michael A. Dojka, Jr.
- 申请人: Gusui Wu , Wessel Lageweg , Maarten H. Stuiver , Lu Liu , Wei Wei , Michael A. Dojka, Jr. , Linda K. Dojka, legal representative
- 申请人地址: US IA Johnston
- 专利权人: Pioneer Hi-Bred International, Inc.
- 当前专利权人: Pioneer Hi-Bred International, Inc.
- 当前专利权人地址: US IA Johnston
- 代理机构: Morrison & Foerster LLP
- 国际申请: PCT/US2004/003378 WO 20040206
- 国际公布: WO2004/072239 WO 20040826
- 主分类号: C07K14/00
- IPC分类号: C07K14/00 ; C07K14/415 ; C12N15/62 ; C12N15/09
摘要:
The invention relates to a method of producing cysteine containing polypeptides in fusion proteins by recombinantly expressing in a host cell sequences encoding an antifungal polypeptide, a maltose binding protein, and a histidine tag. The method is carried out in the presence of a reducing agent to prevent misfolding of the fusion proteins.
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