发明授权
US07998726B2 Method for fixing a protein on a pyrrole-based polymer and use thereof for making a sensor
有权
将蛋白质固定在吡咯类聚合物上的方法及其制造传感器的用途
- 专利标题： Method for fixing a protein on a pyrrole-based polymer and use thereof for making a sensor
- 专利标题（中）： 将蛋白质固定在吡咯类聚合物上的方法及其制造传感器的用途
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申请号： US10533950申请日： 2003-11-20
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公开(公告)号： US07998726B2公开(公告)日： 2011-08-16
- 发明人: Andre Roget , Thierry Livache , Yves Levy
- 申请人： Andre Roget , Thierry Livache , Yves Levy
- 申请人地址： FR Paris FR Paris
- 专利权人： Commissariat a l'Energie Atomique,Centre National de la Recherche Scientifique
- 当前专利权人： Commissariat a l'Energie Atomique,Centre National de la Recherche Scientifique
- 当前专利权人地址： FR Paris FR Paris
- 代理机构： Oblon, Spivak, McClelland, Maier & Neustadt, L.L.P.
- 优先权： FR0214580 20021121
- 国际申请： PCT/FR03/50127 WO 20031120
- 国际公布： WO2004/048972 WO 20040610
- 主分类号： C12M1/34
- IPC分类号： C12M1/34 ; G01N33/53 ; G01N33/551 ; G01N33/544 ; G01N33/547
摘要：
The aim of the present invention is to provide a method for attaching a protein to a conductive polymer, that can be used in particular for producing a sensor or a multisensor, for example a biochip.This aim, and also others, are achieved, in accordance with the invention, by means of a method for attaching a protein to a pyrrole polymer, comprising the following steps: coupling of the protein to be attached with pyrrole so as to obtain a first solution of a protein-pyrrole coupling compound, preparation of a second solution of pyrrole not containing the protein, mixing of said first solution with said second solution so as to obtain an electropolymerization solution, electropolymerization of the pyrrole and of the protein coupled to the pyrrole on a conductive support using said electropolymerization solution.
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