公开(公告)号：US07998726B2

公开(公告)日：2011-08-16

申请号：US10533950

申请日：2003-11-20

申请人： Andre Roget ,  Thierry Livache ,  Yves Levy

发明人： Andre Roget ,  Thierry Livache ,  Yves Levy

IPC分类号： C12M1/34 ,  G01N33/53 ,  G01N33/551 ,  G01N33/544 ,  G01N33/547

CPC分类号： C08G73/0611 ,  C08G61/124 ,  G01N33/54353

摘要： The aim of the present invention is to provide a method for attaching a protein to a conductive polymer, that can be used in particular for producing a sensor or a multisensor, for example a biochip.This aim, and also others, are achieved, in accordance with the invention, by means of a method for attaching a protein to a pyrrole polymer, comprising the following steps: coupling of the protein to be attached with pyrrole so as to obtain a first solution of a protein-pyrrole coupling compound, preparation of a second solution of pyrrole not containing the protein, mixing of said first solution with said second solution so as to obtain an electropolymerization solution, electropolymerization of the pyrrole and of the protein coupled to the pyrrole on a conductive support using said electropolymerization solution.

摘要翻译： 本发明的目的是提供一种将蛋白质连接到导电聚合物上的方法，其特别可用于生产传感器或多传感器，例如生物芯片。 根据本发明，通过将蛋白质与吡咯聚合物连接的方法，其目的以及其它目的以及其它目的，也包括以下步骤：将待连接的蛋白质与吡咯偶联以获得第一 蛋白质 - 吡咯偶联化合物的溶液，制备不含有蛋白质的吡咯的第二溶液，将所述第一溶液与所述第二溶液混合，得到电聚合溶液，吡咯和与吡咯偶联的蛋白质的电聚合 在使用所述电聚合溶液的导电载体上。

公开(公告)号：US20060035208A1

公开(公告)日：2006-02-16

申请号：US10533950

申请日：2003-11-20

申请人： Andre Roget ,  Thierry Livache ,  Yves Levy

发明人： Andre Roget ,  Thierry Livache ,  Yves Levy

IPC分类号： C12Q1/00 ,  G01N33/53 ,  C08G63/91 ,  C08G63/48

CPC分类号： C08G73/0611 ,  C08G61/124 ,  G01N33/54353

摘要： The aim of the present invention is to provide a method for attaching a protein to a conductive polymer, that can be used in particular for producing a sensor or a multisensor, for example a biochip. This aim, and also others, are achieved, in accordance with the invention, by means of a method for attaching a protein to a pyrrole polymer, comprising the following steps: coupling of the protein to be attached with pyrrole so as to obtain a first solution of a protein-pyrrole coupling compound, preparation of a second solution of pyrrole not containing the protein, mixing of said first solution with said second solution so as to obtain an electropolymerization solution, electropolymerization of the pyrrole and of the protein coupled to the pyrrole on a conductive support using said electropolymerization solution.

摘要翻译： 本发明的目的是提供一种将蛋白质连接到导电聚合物上的方法，其特别可用于生产传感器或多传感器，例如生物芯片。 根据本发明，通过将蛋白质与吡咯聚合物连接的方法，其目的以及其它目的以及其它目的，也包括以下步骤：将待连接的蛋白质与吡咯偶联以获得第一 蛋白质 - 吡咯偶联化合物的溶液，不含蛋白质的吡咯的第二溶液的制备，将所述第一溶液与所述第二溶液混合，得到电解聚合溶液，吡咯和与吡咯偶联的蛋白质的电聚合 在使用所述电聚合溶液的导电载体上。

公开(公告)号：US5837859A

公开(公告)日：1998-11-17

申请号：US525506

申请日：1995-09-22

申请人： Robert Teoule ,  Andre Roget ,  Thierry Livache ,  Christelle Barthet ,  Gerard Bidan

发明人： Robert Teoule ,  Andre Roget ,  Thierry Livache ,  Christelle Barthet ,  Gerard Bidan

IPC分类号： C07H19/00 ,  C07H21/00 ,  C08F238/02 ,  C08G61/10 ,  C08G61/12 ,  C08G73/00 ,  C12Q1/68 ,  C25B3/00 ,  C25B11/12 ,  C07H1/02 ,  H05F3/00

CPC分类号： C07H21/00 ,  C08G61/124 ,  C12Q1/6825 ,  B01J2219/00713

摘要： A copolymer of general formula (I), ##STR1## wherein unit A is a monomer of an electronically conductive polymer, unit B is a nucleotide, an oligonucleotide or an analogue thereof, x, y, z are integers of 1 or higher or y is 0, and l is a covalent bond, or a spacer arm. Methods for preparing said polymer and its use, in particular for nucleic acid synthesis, sequencing and hybridization, are also disclosed.

摘要翻译： PCT No.PCT / FR94 / 00354 Sec。 371日期1995年9月22日 102（e）1995年9月22日PCT 1994年3月30日PCT公布。 第WO94 / 22889号公报 日期：1994年10月13日通式（I），其中单元A是电子导电聚合物的单体的通式（I）的共聚物，单元B是核苷酸，寡核苷酸或其类似物，x，y，z 为1以上的整数或y为0，l为共价键或间隔臂。 还公开了制备所述聚合物的方法及其用途，特别是用于核酸合成，测序和杂交。

公开(公告)号：US20060032750A1

公开(公告)日：2006-02-16

申请号：US11155773

申请日：2005-06-20

申请人： Thierry Livache ,  Frederic Lesbre

发明人： Thierry Livache ,  Frederic Lesbre

IPC分类号： C02F1/40

CPC分类号： C09D5/4476 ,  B01J19/0046 ,  B01J2219/00351 ,  B01J2219/00369 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00628 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00713 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

摘要： The invention concerns a method for fabricating matrices of addressed ligands on a carrier. According to this method, an element is used such as a reservoir (1) filled with ligand and containing an electrode (3) to deposit and electrochemically fix the ligand to the conductive carrier (7). The ligand may be an oligonucleotide or a peptide, and fixing may be obtained by electropolymerisation of this oligonucleotide or peptide carrying a pyrrole group at 5′ with pyrrole.

摘要翻译： 本发明涉及一种在载体上制造被寻址配体的基质的方法。 根据该方法，使用诸如填充有配体并且含有电极（3）的储存器（1）的元件以将配体沉积并电化学固定到导电载体（7）。 配体可以是寡核苷酸或肽，并且可以通过将该寡核苷酸或在5'处携带吡咯基团的肽与吡咯电聚合来获得固定。

公开(公告)号：US06607929B1

公开(公告)日：2003-08-19

申请号：US09555136

申请日：2000-07-10

申请人： Thierry Livache ,  Patrice Caillat

发明人： Thierry Livache ,  Patrice Caillat

IPC分类号： H01L2166

CPC分类号： G01R1/06783 ,  G01R31/2829

摘要： A method and device for testing an electronic chip (10) having on the surface a plurality of addressable electrically conductive sensitive elements (12) in which the sensitive elements on the chip are put in contact with a conductive solution (52), one or more sensitive elements (12) are addressed selectively in order to apply to each sensitive element addressed an electrical test signal referred to as an input signal, and a signal, referred to as the output signal, on an electrode (60, 60a) also in contact with the conductive solution, is measured.

摘要翻译： 一种用于测试电子芯片（10）的方法和装置，所述电子芯片（10）在表面上具有多个寻址导电敏感元件（12），其中芯片上的敏感元件与导电溶液（52）接触，一个或多个 选择性地寻址敏感元件（12），以便将被称为输入信号的电测试信号的每个敏感元件和被称为输出信号的信号应用于也接触的电极（60,60a）上 与导电溶液进行测量。

公开(公告)号：US06255677B1

公开(公告)日：2001-07-03

申请号：US09111773

申请日：1998-07-08

申请人： Patrice Caillat ,  Philippe Peltie ,  Thierry Livache

发明人： Patrice Caillat ,  Philippe Peltie ,  Thierry Livache

IPC分类号： H01L2714

CPC分类号： G01N33/53 ,  B01J2219/00495 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00608 ,  B01J2219/00614 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  G01N21/66 ,  G01N27/3276 ,  H05B3/26 ,  H05B2203/002 ,  H05B2203/013

摘要： The invention concerns an analysis device including at least one chip (110) equipped with a plurality of analysis electrodes (112). In accordance with the invention, the device also includes means of individual heating (150) of the analysis electrodes. The invention can be used for the analysis of chemical or biological products, for example in an antigen/antibody recognition or DNA/DNA analysis.

摘要翻译： 本发明涉及包括配备有多个分析电极（112）的至少一个芯片（110）的分析装置。 根据本发明，该装置还包括分析电极单独加热（150）的装置。本发明可用于化学或生物产品的分析，例如在抗原/抗体识别或DNA / DNA分析中。

公开(公告)号：US20140023675A1

公开(公告)日：2014-01-23

申请号：US13977780

申请日：2012-01-03

申请人： Bruno Lina ,  Olivier Ferraris ,  Ho Hong Hai Vo ,  Francois-Loic Cosset ,  Judit Szecsi ,  Alain Heyraud ,  Hugues Lortat-Jacob ,  Julia Bartoli ,  Rabia Sadir ,  Thierry Livache ,  Benoit Darblade ,  Stephane Havet ,  Silvére Bonnet

发明人： Bruno Lina ,  Olivier Ferraris ,  Ho Hong Hai Vo ,  Francois-Loic Cosset ,  Judit Szecsi ,  Alain Heyraud ,  Hugues Lortat-Jacob ,  Julia Bartoli ,  Rabia Sadir ,  Thierry Livache ,  Benoit Darblade ,  Stephane Havet ,  Silvére Bonnet

IPC分类号： A61K31/737

CPC分类号： A61K31/737 ,  A61K31/715 ,  A61K36/05 ,  A61K36/888

摘要： The present invention relates to polysaccharides selected from sulphated arabinogalactans, apiogalacturonans and sulphated heteroglycans intended to be used as a drug for the preventive or curative treatment of an influenza virus, as well as to the pharmaceutical compositions including, in particular in combination with at least one pharmaceutically acceptable carrier: either an extract of Codium fragile, including sulphated arabinogalactans, or an extract of Zostera marina or Lemna minor, including apiogalacturonans, or an extract of Caulerpa racemosa, including sulphated heteroglycans.

摘要翻译： 本发明涉及旨在用作预防或治疗性流感病毒的药物的硫酸化阿拉伯半乳聚糖，阿片半乳糖聚糖和硫酸化异糖聚糖的多糖，以及该药物组合物，特别是与至少一种 药学上可接受的载体：脆弱的Codium的提取物，包括硫酸化的阿拉伯半乳聚糖，或Zostera marina或Lemna minor的提取物，包括半乳糖醛酸聚糖或包括硫酸化杂聚糖的Caulerpa racemosa的提取物。

公开(公告)号：US08147665B2

公开(公告)日：2012-04-03

申请号：US10578318

申请日：2004-11-15

申请人： Thierry Livache ,  Bruno Corso ,  Thibaut Mercey ,  Nathalie Lassalle

发明人： Thierry Livache ,  Bruno Corso ,  Thibaut Mercey ,  Nathalie Lassalle

IPC分类号： G01N27/327

CPC分类号： B01L3/0244 ,  B01L3/0255 ,  B01L2300/0645 ,  B01L2400/025

摘要： The invention relates to a device for receiving a fluid sample, which is designed such as to form an electrode, such as a counter electrode or a working electrode, in an electrochemical cell. The inventive device comprises an end part having at least one cavity which opens to the exterior via an opening and which is equipped with a base. The invention is characterized in that the aforementioned end part comprises a first electrically-insulating hydrophobic zone which is adjacent to the cavity opening and a second electrically-conducting hydrophilic zone which is adjacent to the first zone and which at least partially covers the base of the cavity, such that, when the end part is immersed in the fluid and then removed therefrom, the cavity retains part of the fluid by means of capillary action.

摘要翻译： 本发明涉及一种用于接收流体样品的装置，其被设计成在电化学电池中形成诸如对电极或工作电极之类的电极。 本发明的装置包括具有至少一个空腔的端部，所述至少一个空腔经由开口向外部开口并且具有底座。 本发明的特征在于，上述端部包括与腔开口相邻的第一电绝缘疏水区和邻近第一区的第二导电亲水区，并且至少部分地覆盖第一区的底部 使得当端部浸入流体中然后从其中移出时，空腔通过毛细管作用保持部分流体。

公开(公告)号：US5514540A

公开(公告)日：1996-05-07

申请号：US90436

申请日：1993-07-06

申请人： Robert Teoule ,  Thierry Livache ,  Brigitte Fouque ,  Sylvie Sauvaigo

发明人： Robert Teoule ,  Thierry Livache ,  Brigitte Fouque ,  Sylvie Sauvaigo

IPC分类号： C12Q1/68 ,  C12Q1/70 ,  G01N33/53 ,  C12P19/34

CPC分类号： C12Q1/708 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/686

摘要： A method for detecting specific nucleic acid sequences (a single sequence and/or a mixture of nucleic acid sequences) which are present in a biological sample, comprising at least one enzymatic amplification. Detection is carried out, after placing the biological samples in a solution in order to extract the nucleic acid, by enrichment with target sequences by putting the biological samples into contact with at least one pair of appropriate triggers, followed by at least one appropriate dilution of the obtained enrichment amplifying solution, and by putting a fraction of this solution into contact with at least one pair of triggers, followed by the detection of the obtained double-strand target nucleic acid copies. Applications in the diagnosis of genetic, infectious and tumoral diseases, in checking biological samples and in cell typing etc.

摘要翻译： 存在于生物样品中的特异性核酸序列（单序列和/或核酸序列的混合物）的方法，其包含至少一种酶促扩增。 在将生物样品置于溶液中以提取核酸之后，通过将生物样品与至少一对合适的触发剂接触，然后通过与靶序列的富集至少一次适当的稀释 获得的富集放大溶液，并将该溶液的一部分与至少一对触发剂接触，然后检测所获得的双链靶核酸拷贝。 诊断遗传，感染和肿瘤疾病，检查生物样品和细胞分型等方面的应用

公开(公告)号：US09841429B2

公开(公告)日：2017-12-12

申请号：US13690779

申请日：2012-11-30

申请人： Thierry Livache ,  Yoann Roupioz ,  Sarah Milgram ,  Bernard Maillere ,  Patrice Marche

发明人： Thierry Livache ,  Yoann Roupioz ,  Sarah Milgram ,  Bernard Maillere ,  Patrice Marche

IPC分类号： G01N33/543 ,  G01N33/68 ,  G01N33/50 ,  G01N21/552 ,  B01L3/00

CPC分类号： G01N33/6866 ,  B01L3/5085 ,  G01N21/553 ,  G01N33/5038 ,  G01N33/5044 ,  G01N33/54373 ,  G01N2333/57

摘要： The present invention relates to a method for real-time measurement of the secretion of at least one compound by at least one individual cell, comprising: the culturing, in a liquid medium, of at least one cell in a culture chamber, at least one wall of which comprises at least one sensitive area, a sensitive area comprising a plurality of ligands, attached to a solid support, each ligand being able to bind specifically to the compound, and an element for real-time transduction of a signal produced by the binding of the compound to one of the ligands; the identification, in a sensitive area, of at least one spot producing a signal; the real-time measurement of the signal produced by the spot identified, representing the amount of compound secreted by an individual cell.