发明授权
- 专利标题: Closed-system multi-stage nucleic acid amplification reactions
- 专利标题(中): 封闭系统多级核酸扩增反应
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申请号: US11262523申请日: 2005-10-27
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公开(公告)号: US08133703B2公开(公告)日: 2012-03-13
- 发明人: Jesus Ching , Ronald Chang , Douglas Bryan Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wingkei Wong , Kendra Lara Paul , Reuel Van Atta
- 申请人: Jesus Ching , Ronald Chang , Douglas Bryan Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wingkei Wong , Kendra Lara Paul , Reuel Van Atta
- 申请人地址: US CA Sunnyvale
- 专利权人: Ceoheid
- 当前专利权人: Ceoheid
- 当前专利权人地址: US CA Sunnyvale
- 代理机构: Kilpatrick Towsend & Stockton LLP
- 主分类号: C12P19/34
- IPC分类号: C12P19/34
摘要:
The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.