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公开(公告)号：US08187557B2
公开(公告)日：2012-05-29
申请号：US11485944
申请日：2006-07-13
Applicant: Reuel Van Atta , Dias Lourenco Francisco
Inventor： Reuel Van Atta , Dias Lourenco Francisco
CPC classification number: G01N1/38 , A61B5/0059 , B01L3/502 , B01L3/508 , B01L2200/0647 , B01L2200/185 , Y10T436/25
Abstract: The invention provides a reagent reservoir system and disposable reaction cassettes using the same. In one aspect, such system comprises a chamber in which dried reagent, particularly lyophilized reagent, is constrained to remain in a defined region of the chamber by a retaining member that obstructs passage of such reagents to other regions of the chamber where they may escape hydration or activation.
Abstract translation: 本发明提供一种试剂储存系统和使用其的一次性反应盒。 在一个方面，这种系统包括一个室，其中干燥的试剂，特别是冻干试剂被约束以通过阻挡这些试剂通过室的其他区域的保持构件保持在室的限定区域，在那里它们可以逸出水合 或激活。
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公开(公告)号：US08133703B2
公开(公告)日：2012-03-13
申请号：US11262523
申请日：2005-10-27
Applicant: Jesus Ching , Ronald Chang , Douglas Bryan Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wingkei Wong , Kendra Lara Paul , Reuel Van Atta
Inventor： Jesus Ching , Ronald Chang , Douglas Bryan Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wingkei Wong , Kendra Lara Paul , Reuel Van Atta
IPC: C12P19/34
CPC classification number: G01N21/6428 , B01L3/502 , B01L7/52 , B01L2200/0684 , B01L2300/0654 , B01L2300/0877 , B01L2400/0487 , B01L2400/0644 , C12Q1/6844 , C12Q1/6851 , C12Q1/686 , C12Q2565/629 , C12Q2563/173 , C12Q2549/119 , C12Q2531/143 , C12Q2537/149
Abstract: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
Abstract translation: 本发明涉及用于进行多级扩增反应的系统，方法和装置，特别是在流体封闭的条件下。 在一个方面，本发明的方法在流体封闭的反应体系中进行，其允许分离第一（或先前）反应混合物的一部分，并将其用作第二（或后续）反应混合物中的样品或样品 ，从而基本上避免了如果两个反应混合物简单地组合在一起，第一反应组分可能在第二反应中可能具有干扰效应。 在这方面，本发明的系统，方法和装置可以与基于嵌套引物的使用允许多个扩增阶段的任何扩增反应一起使用。
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公开(公告)号：US20130084631A1
公开(公告)日：2013-04-04
申请号：US13458762
申请日：2012-04-27
Applicant: Reuel Van Atta , Dias Lourenco Francisco
Inventor： Reuel Van Atta , Dias Lourenco Francisco
IPC: B01L3/00
CPC classification number: G01N1/38 , A61B5/0059 , B01L3/502 , B01L3/508 , B01L2200/0647 , B01L2200/185 , Y10T436/25
Abstract: The invention provides a reagent reservoir system and disposable reaction cassettes using the same. In one aspect, such system comprises a chamber in which dried reagent, particularly lyophilized reagent, is constrained to remain in a defined region of the chamber by a retaining member that obstructs passage of such reagents to other regions of the chamber where they may escape hydration or activation.
Abstract translation: 本发明提供一种试剂储存系统和使用其的一次性反应盒。 在一个方面，这种系统包括一个室，其中干燥的试剂，特别是冻干试剂被约束以通过阻挡这些试剂通过室的其他区域的保持构件保持在室的限定区域，在那里它们可以逸出水合 或激活。
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公开(公告)号：US20060177844A1
公开(公告)日：2006-08-10
申请号：US11262523
申请日：2005-10-27
Applicant: Jesus Ching , Ronald Chang , Douglas Dority , Jian Ping Zhang , James Wang , Wendy Wong , Kendra Paul , Reuel Van Atta
Inventor： Jesus Ching , Ronald Chang , Douglas Dority , Jian Ping Zhang , James Wang , Wendy Wong , Kendra Paul , Reuel Van Atta
CPC classification number: G01N21/6428 , B01L3/502 , B01L7/52 , B01L2200/0684 , B01L2300/0654 , B01L2300/0877 , B01L2400/0487 , B01L2400/0644 , C12Q1/6844 , C12Q1/6851 , C12Q1/686 , C12Q2565/629 , C12Q2563/173 , C12Q2549/119 , C12Q2531/143 , C12Q2537/149
Abstract: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
Abstract translation: 本发明涉及用于进行多级扩增反应的系统，方法和装置，特别是在流体封闭的条件下。 在一个方面，本发明的方法在流体封闭的反应体系中进行，其允许分离第一（或先前）反应混合物的一部分，并将其用作第二（或后续）反应混合物中的样品或样品 ，从而基本上避免了如果两个反应混合物简单地组合在一起，第一反应组分可能在第二反应中可能具有干扰效应。 在这方面，本发明的系统，方法和装置可以与基于嵌套引物的使用允许多个扩增阶段的任何扩增反应一起使用。
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Patent Agency Ranking
公开(公告)号：US08728765B2
公开(公告)日：2014-05-20
申请号：US13397416
申请日：2012-02-15
Applicant: Jesus Ching , Ronald Chang , Douglas Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wong , Kendra Lara Paul , Reuel Van Atta
Inventor： Jesus Ching , Ronald Chang , Douglas Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wong , Kendra Lara Paul , Reuel Van Atta
IPC: C12P19/34
CPC classification number: G01N21/6428 , B01L3/502 , B01L7/52 , B01L2200/0684 , B01L2300/0654 , B01L2300/0877 , B01L2400/0487 , B01L2400/0644 , C12Q1/6844 , C12Q1/6851 , C12Q1/686 , C12Q2565/629 , C12Q2563/173 , C12Q2549/119 , C12Q2531/143 , C12Q2537/149
Abstract: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
Abstract translation: 本发明涉及用于进行多级扩增反应的系统，方法和装置，特别是在流体封闭的条件下。 在一个方面，本发明的方法在流体封闭的反应体系中进行，其允许分离第一（或先前）反应混合物的一部分，并将其用作第二（或后续）反应混合物中的样品或样品 ，从而基本上避免了如果两个反应混合物简单地组合在一起，第一反应组分可能在第二反应中可能具有干扰效应。 在这方面，本发明的系统，方法和装置可以与基于嵌套引物的使用允许多个扩增阶段的任何扩增反应一起使用。
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公开(公告)号：US08758701B2
公开(公告)日：2014-06-24
申请号：US13458762
申请日：2012-04-27
Applicant: Reuel Van Atta , Dias Lourenco Francisco
Inventor： Reuel Van Atta , Dias Lourenco Francisco
CPC classification number: G01N1/38 , A61B5/0059 , B01L3/502 , B01L3/508 , B01L2200/0647 , B01L2200/185 , Y10T436/25
Abstract: The invention provides a reagent reservoir system and disposable reaction cassettes using the same. In one aspect, such system comprises a chamber in which dried reagent, particularly lyophilized reagent, is constrained to remain in a defined region of the chamber by a retaining member that obstructs passage of such reagents to other regions of the chamber where they may escape hydration or activation.
Abstract translation: 本发明提供一种试剂储存系统和使用其的一次性反应盒。 在一个方面，这种系统包括一个室，其中干燥的试剂，特别是冻干试剂被约束以通过阻挡这些试剂通过室的其他区域的保持构件保持在室的限定区域，在那里它们可以逸出水合 或激活。
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公开(公告)号：US20120244534A1
公开(公告)日：2012-09-27
申请号：US13397416
申请日：2012-02-15
Applicant: Jesus Ching , Ronald Chang , Douglas Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wingkei Wong , Kendra Lara Paul , Reuel Van Atta
Inventor： Jesus Ching , Ronald Chang , Douglas Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wingkei Wong , Kendra Lara Paul , Reuel Van Atta
CPC classification number: G01N21/6428 , B01L3/502 , B01L7/52 , B01L2200/0684 , B01L2300/0654 , B01L2300/0877 , B01L2400/0487 , B01L2400/0644 , C12Q1/6844 , C12Q1/6851 , C12Q1/686 , C12Q2565/629 , C12Q2563/173 , C12Q2549/119 , C12Q2531/143 , C12Q2537/149
Abstract: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
Abstract translation: 本发明涉及用于进行多级扩增反应的系统，方法和装置，特别是在流体封闭的条件下。 在一个方面，本发明的方法在流体封闭的反应体系中进行，其允许分离第一（或先前）反应混合物的一部分，并将其用作第二（或后续）反应混合物中的样品或样品 ，从而基本上避免了如果两个反应混合物简单地组合在一起，第一反应组分可能在第二反应中可能具有干扰效应。 在这方面，本发明的系统，方法和装置可以与基于嵌套引物的使用允许多个扩增阶段的任何扩增反应一起使用。
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公开(公告)号：US20080014114A1
公开(公告)日：2008-01-17
申请号：US11485944
申请日：2006-07-13
Applicant: Reuel Van Atta , Dias Lourenco Francisco
Inventor： Reuel Van Atta , Dias Lourenco Francisco
IPC: A61L2/00
CPC classification number: G01N1/38 , A61B5/0059 , B01L3/502 , B01L3/508 , B01L2200/0647 , B01L2200/185 , Y10T436/25
Abstract: The invention provides a reagent reservoir system and disposable reaction cassettes using the same. In one aspect, such system comprises a chamber in which dried reagent, particularly lyophilized reagent, is constrained to remain in a defined region of the chamber by a retaining member that obstructs passage of such reagents to other regions of the chamber where they may escape hydration or activation.
Abstract translation: 本发明提供一种试剂储存系统和使用其的一次性反应盒。 在一个方面，这种系统包括一个室，其中干燥的试剂，特别是冻干试剂被约束以通过阻挡这些试剂通过室的其他区域的保持构件保持在室的限定区域，在那里它们可以逸出水合 或激活。
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公开(公告)号：US06187532B1
公开(公告)日：2001-02-13
申请号：US08742376
申请日：1996-11-01
Applicant: Michael Wood , Reuel Van Atta , David Albagli
Inventor： Michael Wood , Reuel Van Atta , David Albagli
IPC: C12Q168
CPC classification number: C12Q1/6827 , Y10S435/81 , C12Q2565/131 , C12Q2523/101 , C12Q2523/313 , C12Q2565/125
Abstract: Double-stranded conformational polymorphism analysis is performed by combining a probe comprising a cross-linking agent and optionally a label with a sample having a target sequence, which may be complementary or have one or a few mismatches with respect to the probe sequence. After sufficient time for hybridization under mild or lesser stringency conditions, hybridized pairs are irradiated to induce cross-link formation by the cross-linking agent. The sample is then analyzed by denaturing gel electrophoresis where the rate of migration depends upon the degree of complementarity between the probe and the target. For corroboration, in a second experiment, the probe may be combined with the sample under high stringency conditions, where it is found that the formation of cross-linked probe/target is substantially lower for pairs having mismatches than for fully matched pairs. After cross-linking, the sample may be separated by gel electrophoresis, and the amount of cross-linked nucleic acid determined.
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