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公开(公告)号:US20120244534A1
公开(公告)日:2012-09-27
申请号:US13397416
申请日:2012-02-15
申请人: Jesus Ching , Ronald Chang , Douglas Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wingkei Wong , Kendra Lara Paul , Reuel Van Atta
发明人: Jesus Ching , Ronald Chang , Douglas Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wingkei Wong , Kendra Lara Paul , Reuel Van Atta
CPC分类号: G01N21/6428 , B01L3/502 , B01L7/52 , B01L2200/0684 , B01L2300/0654 , B01L2300/0877 , B01L2400/0487 , B01L2400/0644 , C12Q1/6844 , C12Q1/6851 , C12Q1/686 , C12Q2565/629 , C12Q2563/173 , C12Q2549/119 , C12Q2531/143 , C12Q2537/149
摘要: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
摘要翻译: 本发明涉及用于进行多级扩增反应的系统,方法和装置,特别是在流体封闭的条件下。 在一个方面,本发明的方法在流体封闭的反应体系中进行,其允许分离第一(或先前)反应混合物的一部分,并将其用作第二(或后续)反应混合物中的样品或样品 ,从而基本上避免了如果两个反应混合物简单地组合在一起,第一反应组分可能在第二反应中可能具有干扰效应。 在这方面,本发明的系统,方法和装置可以与基于嵌套引物的使用允许多个扩增阶段的任何扩增反应一起使用。
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公开(公告)号:US08133703B2
公开(公告)日:2012-03-13
申请号:US11262523
申请日:2005-10-27
申请人: Jesus Ching , Ronald Chang , Douglas Bryan Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wingkei Wong , Kendra Lara Paul , Reuel Van Atta
发明人: Jesus Ching , Ronald Chang , Douglas Bryan Dority , Jian Ping Zhang , James Jian Quan Wang , Wendy Wingkei Wong , Kendra Lara Paul , Reuel Van Atta
IPC分类号: C12P19/34
CPC分类号: G01N21/6428 , B01L3/502 , B01L7/52 , B01L2200/0684 , B01L2300/0654 , B01L2300/0877 , B01L2400/0487 , B01L2400/0644 , C12Q1/6844 , C12Q1/6851 , C12Q1/686 , C12Q2565/629 , C12Q2563/173 , C12Q2549/119 , C12Q2531/143 , C12Q2537/149
摘要: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
摘要翻译: 本发明涉及用于进行多级扩增反应的系统,方法和装置,特别是在流体封闭的条件下。 在一个方面,本发明的方法在流体封闭的反应体系中进行,其允许分离第一(或先前)反应混合物的一部分,并将其用作第二(或后续)反应混合物中的样品或样品 ,从而基本上避免了如果两个反应混合物简单地组合在一起,第一反应组分可能在第二反应中可能具有干扰效应。 在这方面,本发明的系统,方法和装置可以与基于嵌套引物的使用允许多个扩增阶段的任何扩增反应一起使用。
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