In a method for regenerating a biosensor, a biosensor is prepared having a substrate surface on which at least one receptor is immobilized. At least one ligand that is binding specific to the receptor is bound to the receptor, said ligand, together with the receptor, forming a ligand-receptor complex. To regenerate the biosensor, the ligand-receptor complex is brought into contact with an enzyme. The enzyme is selected so that it catalyzes the ligand into fragments. The enzyme is inert with respect to the receptor.