公开(公告)号：US20100129894A1

公开(公告)日：2010-05-27

申请号：US12619871

申请日：2009-11-17

申请人： Sonja Bednar ,  Johannes Baader ,  Holger Klapproth ,  Ingo Freund

发明人： Sonja Bednar ,  Johannes Baader ,  Holger Klapproth ,  Ingo Freund

IPC分类号： C07K1/00

CPC分类号： G01N33/54393

摘要： In a method for regenerating a biosensor, a biosensor is prepared having a substrate surface on which at least one receptor is immobilized. At least one ligand that is binding specific to the receptor is bound to the receptor, said ligand, together with the receptor, forming a ligand-receptor complex. To regenerate the biosensor, the ligand-receptor complex is brought into contact with an enzyme. The enzyme is selected so that it catalyzes the ligand into fragments. The enzyme is inert with respect to the receptor.

摘要翻译： 在用于再生生物传感器的方法中，制备具有固定有至少一种受体的底物表面的生物传感器。 与受体结合特异性的至少一种配体与受体结合，所述配体与受体一起形成配体 - 受体复合物。 为了再生生物传感器，使配体 - 受体复合物与酶接触。 选择该酶以使其将配体催化成片段。 该酶相对于受体是惰性的。

公开(公告)号：US08518709B2

公开(公告)日：2013-08-27

申请号：US12619871

申请日：2009-11-17

申请人： Sonja Bednar ,  Johannes Baader ,  Holger Klapproth ,  Ingo Freund

发明人： Sonja Bednar ,  Johannes Baader ,  Holger Klapproth ,  Ingo Freund

IPC分类号： G01N21/00

CPC分类号： G01N33/54393

摘要： In a method for regenerating a biosensor, a biosensor is prepared having a substrate surface on which at least one receptor is immobilized. At least one ligand that is binding specific to the receptor is bound to the receptor, said ligand, together with the receptor, forming a ligand-receptor complex. To regenerate the biosensor, the ligand-receptor complex is brought into contact with an enzyme. The enzyme is selected so that it catalyzes the ligand into fragments. The enzyme is inert with respect to the receptor.

摘要翻译： 在用于再生生物传感器的方法中，制备具有固定有至少一种受体的底物表面的生物传感器。 与受体结合特异性的至少一种配体与受体结合，所述配体与受体一起形成配体 - 受体复合物。 为了再生生物传感器，使配体 - 受体复合物与酶接触。 选择该酶以使其将配体催化成片段。 该酶相对于受体是惰性的。

公开(公告)号：US08329104B2

公开(公告)日：2012-12-11

申请号：US12146644

申请日：2008-06-26

申请人： Mirko Lehmann ,  Ingo Freund ,  Sonja Mohry ,  Holger Klapproth

发明人： Mirko Lehmann ,  Ingo Freund ,  Sonja Mohry ,  Holger Klapproth

IPC分类号： G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/566 ,  G01N33/543

CPC分类号： G01N33/54373 ,  G01D3/08 ,  G01N27/414 ,  G01N27/4163 ,  G01N33/0032

摘要： A measurement device has at least one sensor for detecting a parameter, a data storage unit, and a power supply for said sensor and data storage unit. In addition to the first sensor, the measurement device has at least one second sensor connected to the power supply and to the data storage unit. The second sensor is provided for detecting a physical quantity acting on the first sensor and is capable of permanently altering the measurement characteristic of the first sensor. An evaluator functions coactively with the second sensor and the data storage unit so that the permanent alteration of the measurement characteristic of the first sensor is recordable as a function of the measurement signal of the second sensor.

摘要翻译： 测量装置具有用于检测参数的至少一个传感器，用于所述传感器和数据存储单元的数据存储单元和电源。 除了第一传感器之外，测量装置具有连接到电源和数据存储单元的至少一个第二传感器。 提供第二传感器用于检测作用在第一传感器上的物理量，并且能够永久地改变第一传感器的测量特性。 评估器与第二传感器和数据存储单元共同作用，使得可以根据第二传感器的测量信号来记录第一传感器的测量特性的永久变化。

公开(公告)号：US20090175764A1

公开(公告)日：2009-07-09

申请号：US12146644

申请日：2008-06-26

申请人： Mirko Lehmann ,  Ingo Freund ,  Sonja Mohry ,  Holger Klapproth

发明人： Mirko Lehmann ,  Ingo Freund ,  Sonja Mohry ,  Holger Klapproth

IPC分类号： B01J19/00

CPC分类号： G01N33/54373 ,  G01D3/08 ,  G01N27/414 ,  G01N27/4163 ,  G01N33/0032

摘要： A measurement device has at least one sensor for detecting a parameter, a data storage unit, and a power supply for said sensor and data storage unit. In addition to the first sensor, the measurement device has at least one second sensor connected to the power supply and to the data storage unit. The second sensor is provided for detecting a physical quantity acting on the first sensor and is capable of permanently altering the measurement characteristic of the first sensor. An evaluator functions coactively with the second sensor and the data storage unit so that the permanent alteration of the measurement characteristic of the first sensor is recordable as a function of the measurement signal of the second sensor.

摘要翻译： 测量装置具有用于检测参数的至少一个传感器，用于所述传感器和数据存储单元的数据存储单元和电源。 除了第一传感器之外，测量装置具有连接到电源和数据存储单元的至少一个第二传感器。 提供第二传感器用于检测作用在第一传感器上的物理量，并且能够永久地改变第一传感器的测量特性。 评估器与第二传感器和数据存储单元共同作用，使得可以根据第二传感器的测量信号来记录第一传感器的测量特性的永久变化。

公开(公告)号：US20050158848A1

公开(公告)日：2005-07-21

申请号：US10511192

申请日：2003-04-10

申请人： Ulrich Sieben ,  Ingo Freund ,  Holger Klapproth

发明人： Ulrich Sieben ,  Ingo Freund ,  Holger Klapproth

IPC分类号： B01J20/32 ,  C09D125/06 ,  C09D179/08 ,  C09D201/00 ,  G01N33/543 ,  G01N37/00 ,  C12M1/34 ,  B05D3/00

CPC分类号： B01J20/3204 ,  B01J20/103 ,  B01J20/28011 ,  B01J20/3257 ,  B01J20/327 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3276 ,  B01J20/3289 ,  G01N33/54393

摘要： In a method for immobilizing molecules on a surface, a layer of a hydrophobic, e.g., a non-swelling polymer, may be applied to the surface and molecules are immobilized on a surface of the polymer layer. The hydrophobic polymers may be polyimide or polystyrene, and the surface to which the polymer layer is applied may be made of an inorganic material, e.g., a semiconductor material (silicon), a semiconducting oxide (silicon dioxide), glass, nitride, or ceramic. Hydrophobic polymers such as polyimide or polystyrene can be applied to the surface of an inorganic support with conventional methods. Further, they electrically insulate the support with respect to the molecules applied to the surface of the polymer layer or substances associated with these molecules. Thus, electrical sensors and processor circuits can be integrated into the support without any detrimental effect on their function due to molecules and substances applied to the surface of the polymer layer.

摘要翻译： 在将分子固定在表面上的方法中，可以将一层疏水的，例如不溶胀的聚合物施加到表面上，并将分子固定在聚合物层的表面上。 疏水聚合物可以是聚酰亚胺或聚苯乙烯，并且施加聚合物层的表面可以由无机材料制成，例如半导体材料（硅），半导体氧化物（二氧化硅），玻璃，氮化物或陶瓷 。 疏水性聚合物如聚酰亚胺或聚苯乙烯可以用常规方法施加到无机载体的表面上。 此外，它们相对于施加到聚合物层的表面上的分子或与这些分子相关联的物质将载体电绝缘。 因此，电传感器和处理器电路可以集成到支撑件中，而不会由于施加到聚合物层表面的分子和物质而对它们的功能产生不利影响。

公开(公告)号：US20060051244A1

公开(公告)日：2006-03-09

申请号：US10528954

申请日：2003-09-26

申请人： Mirko Lehmann ,  Claas Muller ,  Holger Klapproth ,  Ingo Freund

发明人： Mirko Lehmann ,  Claas Muller ,  Holger Klapproth ,  Ingo Freund

IPC分类号： G01N33/50

CPC分类号： G01N21/648 ,  G01N21/552 ,  G01N21/6428 ,  G01N21/6454 ,  G01N33/54373

摘要： Disclosed is a device for detecting at least one ligand contained in a sample that is to be analyzed. Said device comprises an optical waveguide, on the surface of which at least one ligand-specific receptor is directly or indirectly immobilized. The ligand bonds to said receptor during contact therewith. The inventive device comprises at least one optical source of radiation for injecting excitation radiation into the waveguide, the radiation being used for exciting emission of luminescence radiation in accordance with the bonding of the ligand to the receptor. At least one radiation receiver is integrated into the semiconductor substrate of a semiconductor chip so as to detect the luminescence radiation. The waveguide is integrated in a monolithic manner into the semiconductor substrate or is applied thereupon as a wave-guiding layer.

摘要翻译： 公开了一种用于检测待分析样品中所含的至少一种配体的装置。 所述装置包括光波导，其表面上直接或间接固定至少一种配体特异性受体。 配体在与其接触时与所述受体结合。 本发明的装置包括至少一个用于将激发辐射注入到波导中的辐射光源，所述辐射用于根据配体与受体的结合激发发射辐射。 至少一个辐射接收器被集成到半导体芯片的半导体衬底中，以便检测发光辐射。 波导以单片方式集成到半导体衬底中或作为波导层施加在其上。

公开(公告)号：US07462326B2

公开(公告)日：2008-12-09

申请号：US10528954

申请日：2003-09-26

申请人： Mirko Lehmann ,  Claas Müller ,  Holger Klapproth ,  Ingo Freund

发明人： Mirko Lehmann ,  Claas Müller ,  Holger Klapproth ,  Ingo Freund

IPC分类号： G01N21/76 ,  G01N21/00 ,  G01N33/543 ,  G01N21/41 ,  G02B6/12 ,  G02B6/00 ,  G02B6/10 ,  G01B9/02 ,  C12M1/34

CPC分类号： G01N21/648 ,  G01N21/552 ,  G01N21/6428 ,  G01N21/6454 ,  G01N33/54373

摘要： Disclosed is a device for detecting at least one ligand contained in a sample that is to be analyzed. Said device comprises an optical waveguide, on the surface of which at least one ligand-specific receptor is directly or indirectly immobilized. The ligand bonds to said receptor during contact therewith. The inventive device comprises at least one optical source of radiation for injecting excitation radiation into the waveguide, the radiation being used for exciting emission of luminescence radiation in accordance with the bonding of the ligand to the receptor. At least one radiation receiver is integrated into the semiconductor substrate of a semiconductor chip so as to detect the luminescence radiation. The waveguide is integrated in a monolithic manner into the semiconductor substrate or is applied thereupon as a wave-guiding layer.

摘要翻译： 公开了一种用于检测待分析样品中所含的至少一种配体的装置。 所述装置包括光波导，其表面上直接或间接固定至少一种配体特异性受体。 配体在与其接触时与所述受体结合。 本发明的装置包括至少一个用于将激发辐射注入到波导中的辐射光源，所述辐射用于根据配体与受体的结合激发发射辐射。 至少一个辐射接收器被集成到半导体芯片的半导体衬底中，以便检测发光辐射。 波导以单片方式集成到半导体衬底中或作为波导层施加在其上。

公开(公告)号：US20070243637A1

公开(公告)日：2007-10-18

申请号：US11784768

申请日：2007-04-09

申请人： Sonja Mohry ,  Holger Klapproth ,  Mirko Lehmann ,  Ingo Freund

发明人： Sonja Mohry ,  Holger Klapproth ,  Mirko Lehmann ,  Ingo Freund

IPC分类号： G01N33/543 ,  C12M3/00

CPC分类号： G01N33/558 ,  G01N33/54306

摘要： In a process for determining the concentrations of ligands (9, 10) in a sample to be analyzed, ligands (9) of a first ligand type are bond-specific for receptors (6) of a first receptor type, and ligands (10) of a second ligand type are bond-specific for receptors (8) of a second receptor type. A first receptor (6) of the first receptor type is immobilized at least one first test site (5), and a second receptor (8) of the second receptor type is immobilized at a second test site (7) on a substrate (23). A third receptor (11) of the second receptor type is mixed with the sample in such a way that it is present in a specified concentration in the mixture that is obtained in this manner. The mixture is caused to contact the substrate in such a way that at least one ligand (9) of the first ligand type can bind to the first receptor (6) and ligands (10) of the second ligand type can bind to the second receptor (8) and to the third receptor (11). Thereupon components of the mixture that are not bound to an immobilized receptor (6, 8) are removed from the substrate. Thereupon a first measurement signal that is dependent on the concentration of the ligand (9) bound to the first receptor (6) and a second measurement signal that is dependent on the concentration of the ligand (10) bound to the second receptor (8) are generated. With the aid of the first measurement signal the concentration of the ligand (9) of the first ligand type in the sample is determined and, with the aid of the second measurement signal and of the concentration of the third receptor (11) in the mixture, the concentration of the ligands (10) of the second ligand type in the sample is determined.

摘要翻译： 在用于测定待分析样品中配体（9,10）的浓度的方法中，第一配体类型的配体（9）对于第一受体类型的受体（6）是键特异性的，而配体（10） 第二种配体类型是第二受体类型的受体（8）的键特异性。 将第一受体类型的第一受体（6）固定在至少一个第一测试位点（5）上，并且将第二受体类型的第二受体（8）固定在基底（23）上的第二测试位点（7） ）。 将第二受体类型的第三受体（11）与样品混合，使得其以以这种方式获得的混合物中以特定浓度存在。 使混合物以这样的方式接触底物，使得第一配体型的至少一种配体（9）可以结合第一受体（6），并且第二配体型的配体（10）可以结合第二受体 （8）和第三受体（11）。 因此，从底物中除去不与固定化受体（6,8）结合的混合物的组分。 因此，依赖于与第一受体（6）结合的配体（9）的浓度的第一测量信号和取决于与第二受体（8）结合的配体（10）的浓度的第二测量信号， 被生成。 借助于第一测量信号，测定样品中第一配体类型的配体（9）的浓度，借助于第二测量信号和混合物中第三受体（11）的浓度 测定样品中第二配体类型的配体（10）的浓度。

公开(公告)号：US20080138829A1

公开(公告)日：2008-06-12

申请号：US11665169

申请日：2005-10-12

申请人： Mirko Lehmann ,  Ingo Freund ,  Holger Klapproth ,  Sonja Mohry

发明人： Mirko Lehmann ,  Ingo Freund ,  Holger Klapproth ,  Sonja Mohry

IPC分类号： G01N21/76 ,  G01N33/543 ,  G01N33/53

CPC分类号： G01N33/54373

摘要： The invention relates to a method for detecting and/or determining the concentration of a ligand contained in a solution to be analyzed, during which a receptor, which can enter into a specific bond with the ligand, is immobilized on a semiconductor chip at a test location. In order to bind the ligand to the receptor, the solution is applied to the test location. A luminescence radiation or a color change is generated according to the binding of the ligand to the receptor. A radiation measurement signal is picked up with the aid of a radiation receiver integrated in the semiconductor chip, whereas the solution and/or an auxiliary liquid that does not contain the luminophore is in contact with the radiation receiver. While the generation of luminescence radiation is prevented and the radiation receiver is in contact with the auxiliary liquid and/or with a substitute liquid, a darkness measurement signal is measured for the radiation receiver. The radiation measurement signal is compensated for with the darkness measurement signal

摘要翻译： 本发明涉及一种用于检测和/或确定待分析溶液中所含配体的浓度的方法，在该方法中，可以与配体进入特定键的受体在测试中固定在半导体芯片上 位置。 为了将配体结合到受体，将溶液施加到测试位置。 根据配体与受体的结合产生发光辐射或颜色变化。 借助于集成在半导体芯片中的辐射接收器拾取辐射测量信号，而不包含发光体的溶液和/或辅助液体与辐射接收器接触。 虽然防止了发光辐射的产生并且辐射接收器与辅助液体和/或替代液体接触，但是对辐射接收器测量了暗度测量信号。 用黑度测量信号补偿辐射测量信号

公开(公告)号：US20050070009A1

公开(公告)日：2005-03-31

申请号：US10495845

申请日：2002-11-12

申请人： Holger Klapproth ,  Mirko Lehmann ,  Ingo Freund ,  Joachim Sturken

发明人： Holger Klapproth ,  Mirko Lehmann ,  Ingo Freund ,  Joachim Sturken

IPC分类号： B01J19/00 ,  C12M1/34 ,  C08G63/48 ,  C08G63/91

CPC分类号： B01J19/0046

摘要： The invention concerns a biopolymer synthesis substrate, its use and a method for producing biopolymers

摘要翻译： 本发明涉及生物聚合物合成底物，其用途和用于生产生物聚合物的方法