公开(公告)号：US08685644B2

公开(公告)日：2014-04-01

申请号：US10515535

申请日：2003-11-04

申请人： Holger Klapproth

发明人： Holger Klapproth

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00

CPC分类号： G01N33/54373 ,  B01L3/5027 ,  G01N27/4145 ,  G01N2035/00158

摘要： The invention relates to a method for determining a concentration of ligands in an analyzed sample. The inventive method consists in immobilizing receptors specifically boundable with said ligands on a support. At least one measured value of the occupation of the support surface by the receptors is determined by means of a sensor for the surface occupation. Afterwards, the sample is put in contact with the receptors. At least one measured value of the frequency of liaisons between the ligands and the receptors is determined by means of at least one detector. The concentration of ligands in the sample can be determined with the aid of the measured values of the occupation of the surface and frequency of ligands/receptors liaisons.

摘要翻译： 本发明涉及一种用于测定分析样品中配体浓度的方法。 本发明的方法包括将与所述配体特异性结合的受体固定在载体上。 通过用于表面占据的传感器来确定由受体对支撑表面的占据的至少一个测量值。 然后将样品与受体接触。 通过至少一个检测器确定配体和受体之间的联络频率的至少一个测量值。 样品中配体的浓度可以借助于配体/受体结构的表面和频率的占据的测量值来确定。

公开(公告)号：US08518709B2

公开(公告)日：2013-08-27

申请号：US12619871

申请日：2009-11-17

申请人： Sonja Bednar ,  Johannes Baader ,  Holger Klapproth ,  Ingo Freund

发明人： Sonja Bednar ,  Johannes Baader ,  Holger Klapproth ,  Ingo Freund

IPC分类号： G01N21/00

CPC分类号： G01N33/54393

摘要： In a method for regenerating a biosensor, a biosensor is prepared having a substrate surface on which at least one receptor is immobilized. At least one ligand that is binding specific to the receptor is bound to the receptor, said ligand, together with the receptor, forming a ligand-receptor complex. To regenerate the biosensor, the ligand-receptor complex is brought into contact with an enzyme. The enzyme is selected so that it catalyzes the ligand into fragments. The enzyme is inert with respect to the receptor.

摘要翻译： 在用于再生生物传感器的方法中，制备具有固定有至少一种受体的底物表面的生物传感器。 与受体结合特异性的至少一种配体与受体结合，所述配体与受体一起形成配体 - 受体复合物。 为了再生生物传感器，使配体 - 受体复合物与酶接触。 选择该酶以使其将配体催化成片段。 该酶相对于受体是惰性的。