发明授权
US08574835B2 Scaffolded nucleic acid polymer particles and methods of making and using
有权
支架核酸聚合物颗粒及其制备和使用方法
- 专利标题: Scaffolded nucleic acid polymer particles and methods of making and using
- 专利标题(中): 支架核酸聚合物颗粒及其制备和使用方法
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申请号: US12785685申请日: 2010-05-24
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公开(公告)号: US08574835B2公开(公告)日: 2013-11-05
- 发明人: Wolfgang Hinz , John Leamon , David Light , Jonathan Rothberg
- 申请人: Wolfgang Hinz , John Leamon , David Light , Jonathan Rothberg
- 申请人地址: US CA Carlsbad
- 专利权人: Life Technologies Corporation
- 当前专利权人: Life Technologies Corporation
- 当前专利权人地址: US CA Carlsbad
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12M1/00 ; C12M1/34 ; C12M3/00
摘要:
The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.