公开(公告)号：US09534092B2

公开(公告)日：2017-01-03

申请号：US13371264

申请日：2012-02-10

申请人： Wolfgang Hinz ,  Ryan Jones ,  David Light

发明人： Wolfgang Hinz ,  Ryan Jones ,  David Light

IPC分类号： B07B1/00 ,  C08J3/12 ,  C12N15/10

CPC分类号： C08J3/12 ,  B07B1/00 ,  C08J2300/14 ,  C12N15/1017

摘要： A method of preparing hydrogel particles includes applying a solution including a plurality of hydrogel particles to a stir cell. A retentate side of a filter defines a lower surface of the stir cell. The filter has the retentate side and a permeate side. The method further includes, while stirring the solution within the stir cell, dispensing a buffer solution at a first flow rate to the stir cell and drawing a permeate from the permeate side of the filter using a pump at a second flow rate, the permeate including a subset of the plurality of hydrogel particles.

摘要翻译： 制备水凝胶颗粒的方法包括将包含多个水凝胶颗粒的溶液施加到搅拌槽。 过滤器的滞留侧限定了搅拌槽的下表面。 过滤器具有滞留侧和渗透侧。 该方法还包括在搅拌槽内搅拌溶液的同时，以第一流速向缓冲池分配缓冲溶液，并以第二流量使用泵从过滤器的渗透侧抽出渗透物，渗透物包括 多个水凝胶颗粒的一个子集。

公开(公告)号：US20120094871A1

公开(公告)日：2012-04-19

申请号：US13027355

申请日：2011-02-15

申请人： Wolfgang HINZ ,  John Leamon ,  David Light ,  Jonathan Rothberg

发明人： Wolfgang HINZ ,  John Leamon ,  David Light ,  Jonathan Rothberg

IPC分类号： C40B40/06

CPC分类号： C12N15/1093 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

摘要： The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

摘要翻译： 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中粒子与粒子变化的减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。

公开(公告)号：US07737145B2

公开(公告)日：2010-06-15

申请号：US11644244

申请日：2006-12-22

申请人： Damian Arnaiz ,  Greg Brown ,  Emmanuel Claret ,  Arwed Cleve ,  David Davey ,  William Guilford ,  Seock-Kyu Khim ,  Thomas Kirkland ,  Monica J. Kochanny ,  Amy Liang ,  David Light ,  John Parkinson ,  David Vogel ,  Guo Ping Wei ,  Bin Ye

发明人： Damian Arnaiz ,  Greg Brown ,  Emmanuel Claret ,  Arwed Cleve ,  David Davey ,  William Guilford ,  Seock-Kyu Khim ,  Thomas Kirkland ,  Monica J. Kochanny ,  Amy Liang ,  David Light ,  John Parkinson ,  David Vogel ,  Guo Ping Wei ,  Bin Ye

IPC分类号： A01N43/60 ,  A61K31/495 ,  C07D241/36 ,  C07D271/00 ,  C07D487/00 ,  C07D491/00 ,  C07D495/00 ,  C07D497/00 ,  C07D498/00 ,  C07D513/00

CPC分类号： C07D487/08 ,  C07B2200/07 ,  C07C217/58 ,  C07C229/08 ,  C07C229/38 ,  C07C233/78 ,  C07C255/57 ,  C07C271/20 ,  C07C2601/14 ,  C07D207/04 ,  C07D207/09 ,  C07D207/16 ,  C07D211/26 ,  C07D211/34 ,  C07D211/58 ,  C07D243/08 ,  C07D263/14 ,  C07D263/32 ,  C07D277/22 ,  C07D277/68 ,  C07D295/04 ,  C07D295/096 ,  C07D295/16 ,  C07D401/10 ,  C07D401/12 ,  C07D403/12 ,  C07D405/12 ,  C07D409/12 ,  C07D413/12 ,  C07D451/02 ,  C07D451/04

摘要： This invention is directed to compounds of formula (I): where r, q, R, R2, R3, R4, R5a, R5b, R5c, R6a, R6b, R6c, R7, R8, and R9 are described herein, as single stereoisomers or as mixtures of stereoisomers, or pharmaceutically acceptable salts, solvates, clathrates, polymorphs, ammonium ions, N-oxides or prodrugs thereof; which are leukotriene A4 hydrolase inhibitors and therefore useful in treating inflammatory disorders. Pharmaceutical compositions comprising the compounds of the invention and methods of preparing the compounds of the invention are also disclosed.

摘要翻译： 本发明涉及式（I）化合物：其中r，q，R，R2，R3，R4，R5a，R5b，R5c，R6a，R6b，R6c，R7，R8和R9在本文中描述为单立体异构体 或作为立体异构体或其药学上可接受的盐，溶剂合物，包合物，多晶型物，铵离子，N-氧化物或前药的混合物; 其是白三烯A4水解酶抑制剂，因此可用于治疗炎症性疾病。 还公开了包含本发明化合物的药物组合物和制备本发明化合物的方法。

公开(公告)号：US20070155727A1

公开(公告)日：2007-07-05

申请号：US11644822

申请日：2006-12-22

申请人： Ming Chen ,  Emmanuel Claret ,  Arwed Cleve ,  David Davey ,  William Guilford ,  Seock-Kyu Khim ,  Thomas Kirkland ,  Monica Kochanny ,  Amy Liang ,  David Light ,  John Parkinson ,  David Vogel ,  Guo Wei ,  Bin Ye ,  Hong Ye

发明人： Ming Chen ,  Emmanuel Claret ,  Arwed Cleve ,  David Davey ,  William Guilford ,  Seock-Kyu Khim ,  Thomas Kirkland ,  Monica Kochanny ,  Amy Liang ,  David Light ,  John Parkinson ,  David Vogel ,  Guo Wei ,  Bin Ye ,  Hong Ye

IPC分类号： A61K31/551 ,  A61K31/407 ,  C07D487/02 ,  C07D243/08

CPC分类号： C07D207/04 ,  C07C237/04 ,  C07C237/06 ,  C07C237/30 ,  C07C255/58 ,  C07C275/40 ,  C07C275/42 ,  C07C275/62 ,  C07C311/05 ,  C07C311/08 ,  C07C311/09 ,  C07C311/37 ,  C07C2601/08 ,  C07C2601/14 ,  C07D211/06 ,  C07D243/08 ,  C07D295/04 ,  C07D295/16 ,  C07D487/02 ,  C07D487/08

摘要： This invention is directed to compounds of formula (I): where r, q, R, R2, R3, R4a, R4b, R4c, R5a, R5b, R5c, R6a, R6b, R6c, R7, R8, R9 and R10 are described herein, or pharmaceutically acceptable salts, solvates, polymorphs, ammonium ions, N-oxides or prodrugs thereof; which are leukotriene A4 hydrolase inhibitors and which are therefore useful in treating inflammatory disorders. Pharmaceutical compositions comprising the compounds of the invention and methods of using and preparing the compounds of the invention are also disclosed.

摘要翻译： 本发明涉及式（I）化合物：其中r，q，R，R 2，R 3，R 4a，R 4， R 4b，R 4c，R 5a，R 5b，R 5c，R 5，R 5， R 6b，R 6b，R 6，R 7，R 8，R 8，R 8， 本文描述了本发明的其它药学上可接受的盐，溶剂合物，多晶型物，铵离子，N-氧化物或前体药物; 其是白三烯A 4水解酶抑制剂，因此其可用于治疗炎症性疾病。 还公开了包含本发明化合物的药物组合物和使用和制备本发明化合物的方法。

公开(公告)号：US07152311B2

公开(公告)日：2006-12-26

申请号：US10032886

申请日：2001-12-26

申请人： Masud Beroz ,  Thomas H. DiStefano ,  Matthew T. Hendrickson ,  David Light ,  John W. Smith

发明人： Masud Beroz ,  Thomas H. DiStefano ,  Matthew T. Hendrickson ,  David Light ,  John W. Smith

IPC分类号： B23P19/04

CPC分类号： H01L24/86 ,  B29C2043/5825 ,  H01L21/67144 ,  H01L2224/7965 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01011 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01019 ,  H01L2924/01029 ,  H01L2924/01033 ,  H01L2924/01042 ,  H01L2924/01078 ,  H01L2924/01079 ,  H01L2924/01082 ,  H01L2924/12042 ,  H01L2924/30105 ,  Y10S269/909 ,  Y10T29/53265 ,  Y10T29/53435 ,  Y10T428/23 ,  Y10T428/239 ,  H01L2924/00

摘要： A flexible sheet used in manufacture of microelectronic components is held on a frame formed from a rigid material so that the frame maintains the sheet under tension during processing and thereby stabilizes the dimensions of the sheet. The frame may be formed from a rigid, light-transmissive material such as a glass, and the bond between the frame and sheet may be made or released by light transmitted through the frame. Preferred features of the framed sheet minimize entrapment of processing liquids such as etch solutions, thereby minimizing carryover of processing solutions between steps. The frame may have contact openings which permit engagement of a metallic layer on the sheet by an electrode carrying electroplating or etching current without disturbing the main portion of the sheet where features are to be formed or treated.

公开(公告)号：US20060275951A1

公开(公告)日：2006-12-07

申请号：US11487880

申请日：2006-07-17

申请人： Michael Warner ,  Masud Beroz ,  David Light ,  Delin Li ,  Dennis Castillo ,  Hung-ming Wang ,  John Smith

发明人： Michael Warner ,  Masud Beroz ,  David Light ,  Delin Li ,  Dennis Castillo ,  Hung-ming Wang ,  John Smith

IPC分类号： H01L21/00

CPC分类号： H01L24/10 ,  H01L23/3128 ,  H01L23/3135 ,  H01L23/49816 ,  H01L23/49827 ,  H01L23/4985 ,  H01L23/5389 ,  H01L24/13 ,  H01L2224/05001 ,  H01L2224/05022 ,  H01L2224/05572 ,  H01L2224/13 ,  H01L2224/13022 ,  H01L2224/13099 ,  H01L2224/73204 ,  H01L2224/81193 ,  H01L2224/8193 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01022 ,  H01L2924/01024 ,  H01L2924/01029 ,  H01L2924/01033 ,  H01L2924/01079 ,  H01L2924/01082 ,  H01L2924/01322 ,  H01L2924/014 ,  H01L2924/00 ,  H01L2224/05644 ,  H01L2924/00014 ,  H01L2224/05647 ,  H01L2224/05655 ,  H01L2224/05666 ,  H01L2224/05671 ,  H01L2224/05124

摘要： A microelectronic assembly includes a first microelectronic element having a first face and contacts accessible at the first face, and a layer of a dielectric material having a bottom surface contacting the first microelectronic element, a top surface facing away from the first microelectronic element and holes extending between the top and bottom faces in alignment with the contacts on the first microelectronic element. The assembly includes conductive protrusions extending through the holes to the contacts, the conductive protrusions projecting beyond the top surface of the dielectric layer. The assembly also has a second microelectronic element having a first surface with conductive elements being accessible at the first surface thereof, the second microelectronic element being assembled with the first microelectronic element so that the contacts of the second microelectronic element are connected with the conductive protrusions and the top surface of the dielectric layer is spaced from the first surface of the second microelectronic element.

摘要翻译： 微电子组件包括具有第一面和在第一面可接触的触点的第一微电子元件和具有接触第一微电子元件的底表面的电介质材料层，背离第一微电子元件的顶表面和延伸 在顶面和底面之间，与第一微电子元件上的触点对齐。 组件包括延伸穿过孔到触头的导电突起，导电突出部突出超过电介质层的顶表面。 组件还具有第二微电子元件，其具有第一表面，其导电元件在其第一表面处可访问，第二微电子元件与第一微电子元件组装，使得第二微电子元件的触点与导电突起连接， 电介质层的顶表面与第二微电子元件的第一表面间隔开。

公开(公告)号：US20050014783A1

公开(公告)日：2005-01-20

申请号：US10857062

申请日：2004-05-28

申请人： William Dole ,  David Light ,  Baby Martin-McNulty ,  Sabine Schirm ,  Yi-Xin Wang

发明人： William Dole ,  David Light ,  Baby Martin-McNulty ,  Sabine Schirm ,  Yi-Xin Wang

IPC分类号： A61K31/4409 ,  A61K31/47 ,  A61K31/4725 ,  A61K31/551 ,  A61K31/5513 ,  A61P9/00 ,  A61P9/10 ,  C07D401/12

CPC分类号： C07D401/12

摘要： The present invention is directed to a method for preventing or decreasing the incidence of or treating aneurysm or cardiac hypertrophy, comprising administering an effective amount of a rho-kinase inhibitor, such as fasudil, to a subject in need thereof.

摘要翻译： 本发明涉及一种用于预防或减少动脉瘤或心脏肥大的发生或治疗的方法，包括向有需要的受试者施用有效量的rho激酶抑制剂，如法舒地尔。

公开(公告)号：US06790828B2

公开(公告)日：2004-09-14

申请号：US10438648

申请日：2003-05-14

申请人： David Light ,  Michael John Morser ,  Mariko Nagashima

发明人： David Light ,  Michael John Morser ,  Mariko Nagashima

IPC分类号： A61K3816

CPC分类号： C07K14/7455 ,  A61K38/00

摘要： The present invention relates to the design, production and use of analogs of thrombomodulin (TM) that have the ability to enhance the thrombin-mediated activation of protein C but which have a significantly reduced ability to promote activation of thrombin-activatable fibrinolysis inhibitor (TAFI). These analogs are useful in, for example, antithrombotic therapy.

摘要翻译： 本发明涉及血栓调节蛋白（TM）类似物的设计，生产和使用，其具有增强凝血酶介导的蛋白质C活化但能显着降低促凝血酶激活性纤维蛋白溶解抑制剂（TAFI）活化能力的能力 ）。 这些类似物在例如抗血栓治疗中是有用的。

公开(公告)号：US08574835B2

公开(公告)日：2013-11-05

申请号：US12785685

申请日：2010-05-24

申请人： Wolfgang Hinz ,  John Leamon ,  David Light ,  Jonathan Rothberg

发明人： Wolfgang Hinz ,  John Leamon ,  David Light ,  Jonathan Rothberg

IPC分类号： C12Q1/68 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00

CPC分类号： B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12N15/1093 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

摘要： The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

摘要翻译： 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中粒子与粒子变化的减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。

公开(公告)号：US20110318820A1

公开(公告)日：2011-12-29

申请号：US13172048

申请日：2011-06-29

申请人： Wolfgang Hinz ,  David Light ,  Todd Rearick ,  James Ball

发明人： Wolfgang Hinz ,  David Light ,  Todd Rearick ,  James Ball

IPC分类号： C12M1/34

CPC分类号： C12Q1/6874 ,  G01N27/4145

摘要： The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.

摘要翻译： 本公开涉及新型颗粒组合物和制备所述组合物在核酸分析中的应用的方法，以及用于其的装置和系统。