发明授权
- 专利标题： Nucleic acid amplification in the presence of modified randomers
- 专利标题（中）： 在改良的随机存在下进行核酸扩增
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申请号： US13711846申请日： 2012-12-12
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公开(公告)号： US08592184B2公开(公告)日： 2013-11-26
- 发明人: Dieter Heindl , Waltraud Ankenbauer , Frank Laue
- 申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
- 申请人地址： US IN Indianapolis
- 专利权人： Roche Diagnostics Operations, Inc.
- 当前专利权人： Roche Diagnostics Operations, Inc.
- 当前专利权人地址： US IN Indianapolis
- 代理机构： Dinsmore & Shohl LLP
- 优先权： EP08005100 20080319
- 主分类号： C12P19/34
- IPC分类号： C12P19/34 ; C07H21/00
摘要：
The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.