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公开(公告)号：US20090269766A1
公开(公告)日：2009-10-29
申请号：US12406450
申请日：2009-03-18
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
CPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/686 , C12Q2525/179 , C12Q2525/101
摘要： The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.
摘要翻译： 本发明涉及包含优选热稳定性的DNA聚合酶，脱氧核苷酸，至少一种引物寡核苷酸或一对扩增引物和随机化的5-8位寡核苷酸的组合物，其特征在于所述寡核苷酸包含与有机 疏水部分这样的组合物特别适用于进行热启动PCR。
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公开(公告)号：US20090181401A1
公开(公告)日：2009-07-16
申请号：US12407339
申请日：2009-03-19
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q2561/113 , C12Q2531/113 , C12Q2521/319
摘要： The present invention is directed to a method and a composition for amplifying and detecting a target nucleic comprising subjecting said target nucleic acid to a real time PCR amplification reaction in the presence of a thermostable DNA polymerase, a thermostable double strand dependent 3′-5′ exonuclease having a temperature optimum above 37° C., a pair of amplification primers, deoxynucleoside triphosphates, a detecting oligonucleotide carrying a first label and a second label, said first label being capable of acting as a fluorescent reporter entity when excited with light of an appropriate wavelength, said second label being capable of acting as a fluorescence quenching entity of said fluorescent reporter entity, characterized in that one label is bound to the 3′ end of said detecting oligonucleotide, and further characterized in that the other label is bound either internally or at the 5′ end of said detecting oligonucleotide, and monitoring fluorescence of said fluorescent reporter entity at least after a plurality of amplification cycles.
摘要翻译： 本发明涉及用于扩增和检测靶核酸的方法和组合物，其包括在热稳定性DNA聚合酶，耐热双链依赖性3'-5'的存在下使所述靶核酸进行实时PCR扩增反应， 温度最高在37℃以上的外切核酸酶，一对扩增引物，脱氧核苷三磷酸，携带第一标记和第二标记的检测寡核苷酸，所述第一标记当用 所述第二标记能够用作所述荧光报道实体的荧光猝灭实体，其特征在于一个标记与所述检测寡核苷酸的3'末端结合，其特征还在于另一个标记在内部结合 或在所述检测寡核苷酸的5'末端，并监测所述荧光报告的荧光 至少在多个放大循环之后。
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公开(公告)号：US20050037410A1
公开(公告)日：2005-02-17
申请号：US10903992
申请日：2004-07-30
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
CPC分类号： C12Q1/6818 , C12Q2561/113 , C12Q2531/113 , C12Q2521/319
摘要： The present invention is directed to a method and a composition for amplifying and detecting a target nucleic comprising subjecting said target nucleic acid to a real time PCR amplification reaction in the presence of a thermostable DNA polymerase, a thermostable double strand dependent 3′-5′ exonuclease having a temperature optimum above 37° C., a pair of amplification primers, deoxynucleoside triphosphates, a detecting oligonucleotide carrying a first label and a second label, said first label being capable of acting as a fluorescent reporter entity when excited with light of an appropriate wavelength, said second label being capable of acting as a fluorescence quenching entity of said fluorescent reporter entity, characterized in that one label is bound to the 3′ end of said detecting oligonucleotide, and further characterized in that the other label is bound either internally or at the 5′ end of said detecting oligonucleotide, and monitoring fluorescence of said fluorescent reporter entity at least after a plurality of amplification cycles.
摘要翻译： 本发明涉及用于扩增和检测靶核酸的方法和组合物，其包括在热稳定性DNA聚合酶，耐热双链依赖性3'-5'的存在下使所述靶核酸进行实时PCR扩增反应， 温度最高在37℃以上的外切核酸酶，一对扩增引物，脱氧核苷三磷酸，携带第一标记和第二标记的检测寡核苷酸，所述第一标记当用 所述第二标记能够用作所述荧光报道实体的荧光猝灭实体，其特征在于一个标记与所述检测寡核苷酸的3'末端结合，其特征还在于另一个标记在内部结合 或在所述检测寡核苷酸的5'末端，并监测所述荧光报告的荧光 至少在多个放大循环之后。
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公开(公告)号：US20130109060A1
公开(公告)日：2013-05-02
申请号：US13711846
申请日：2012-12-12
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/686 , C12Q2525/179 , C12Q2525/101
摘要： The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.
摘要翻译： 本发明涉及包含优选热稳定性的DNA聚合酶，脱氧核苷酸，至少一种引物寡核苷酸或一对扩增引物和随机化的5-8位寡核苷酸的组合物，其特征在于所述寡核苷酸包含与有机 疏水部分这样的组合物特别适用于进行热启动PCR。
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公开(公告)号：US07910720B2
公开(公告)日：2011-03-22
申请号：US12547013
申请日：2009-08-25
申请人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Eva Walter , Renate Kolb
发明人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Eva Walter , Renate Kolb
CPC分类号： C07H21/00 , C12Q1/6848 , C12Q2527/125
摘要： The present invention is directed to a novel chemical compound comprising the structure [Xx-(CH2)m-phosphate-Yy]n, characterized in that 3≦m≦6, 30≦n≦60, each x and y is independently from each other 0 or 1, each X and Y is independently from each other any photometrically measurable entity; provided that the terminal X can also be an —OH group or a phosphate group, and further provided that the terminal Y can also be an —OH group. Such a compound can be used as a suitable hot start additive for PCR based amplification of nucleic acids.
摘要翻译： 本发明涉及一种包含结构[Xx-（CH2）m-磷酸-Yy] n结构的新化合物，其特征在于，3和nlE; m＆nlE; 6,30＆lt; n; n＆nlE; 60，每个x和y各自独立地表示 其他0或1，每个X和Y彼此独立地具有任何光度测量实体; 条件是末端X也可以是-OH基或磷酸基，并且进一步规定，末端Y也可以是-OH基。 这种化合物可以用作基于PCR扩增核酸的合适的热启动添加剂。
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公开(公告)号：US07122355B2
公开(公告)日：2006-10-17
申请号：US10192902
申请日：2002-07-11
申请人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Andreas Huber
发明人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Andreas Huber
IPC分类号： C12P19/34
CPC分类号： C12Q1/6848 , C12Q1/6853 , C12Q2549/101 , C12Q2525/186 , C12Q2521/319
摘要： The present invention is directed to a new composition for performing a nucleic acid amplification reaction comprising (i) a thermostable DNA-Polymerase, (ii) a thermostable 3′-5′ Exonuclease, and (iii) at least one primer for nucleic acid amplification with a modified 3′ terminal residue which is not elongated by said thermostable DNA-Polymerase as well as methods for performing a PCR reaction using this composition. Furthermore, the method is directed to kits comprising such a composition.
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公开(公告)号：US08592184B2
公开(公告)日：2013-11-26
申请号：US13711846
申请日：2012-12-12
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
CPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/686 , C12Q2525/179 , C12Q2525/101
摘要： The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.
摘要翻译： 本发明涉及包含优选热稳定性的DNA聚合酶，脱氧核苷酸，至少一种引物寡核苷酸或一对扩增引物和随机化的5-8位寡核苷酸的组合物，其特征在于所述寡核苷酸包含与有机 疏水部分这样的组合物特别适用于进行热启动PCR。
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公开(公告)号：US08026058B2
公开(公告)日：2011-09-27
申请号：US12198966
申请日：2008-08-27
申请人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue
发明人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue
CPC分类号： C12Q1/6848 , C12Q2549/101 , C12Q2527/125
摘要： The present invention is directed to a synthetic peptide having a length of not more than 30 amino acids comprising a divalent cation binding site. Such a peptide according to the present invention is part of a composition for nucleic acid amplification and provides for a so-called hot start effect.
摘要翻译： 本发明涉及长度不超过30个氨基酸的包含二价阳离子结合位点的合成肽。 根据本发明的这种肽是用于核酸扩增的组合物的一部分，并提供所谓的热启动效应。
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公开(公告)号：US20100285535A1
公开(公告)日：2010-11-11
申请号：US12198966
申请日：2008-08-27
申请人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue
发明人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue
CPC分类号： C12Q1/6848 , C12Q2549/101 , C12Q2527/125
摘要： The present invention is directed to a synthetic peptide having a length of not more than 30 amino acids comprising a divalent cation binding site. Such a peptide according to the present invention is part of a composition for nucleic acid amplification and provides for a so-called hot start effect.
摘要翻译： 本发明涉及长度不超过30个氨基酸的包含二价阳离子结合位点的合成肽。 根据本发明的这种肽是用于核酸扩增的组合物的一部分，并提供所谓的热启动效应。
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公开(公告)号：US20100255547A1
公开(公告)日：2010-10-07
申请号：US12547013
申请日：2009-08-25
申请人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Eva Walter , Renate Kolb
发明人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Eva Walter , Renate Kolb
CPC分类号： C07H21/00 , C12Q1/6848 , C12Q2527/125
摘要： The present invention is directed to a novel chemical compound comprising the structure [Xx-(CH2)m-phosphate-Yy]n, characterized in that 3≦m≦6, 30≦n≦60, each x and y is independently from each other 0 or 1, each X and Y is independently from each other any photometrically measurable entity; provided that the terminal X can also be an —OH group or a phosphate group, and further provided that the terminal Y can also be an —OH group. Such a compound can be used as a suitable hot start additive for PCR based amplification of nucleic acids.
摘要翻译： 本发明涉及一种包含结构[Xx-（CH2）m-磷酸-Yy] n结构的新化合物，其特征在于，3和nlE; m＆nlE; 6,30＆lt; n; n＆nlE; 60，每个x和y各自独立地表示 其他0或1，每个X和Y彼此独立地具有任何光度测量实体; 条件是末端X也可以是-OH基或磷酸基，并且进一步规定，末端Y也可以是-OH基。 这种化合物可以用作基于PCR扩增核酸的合适的热启动添加剂。
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