发明授权
US08834893B2 Nucleic acid derived from hepatitis C virus and expression vector, transformed cell, and hepatitis C virus particles each prepared by using the same
有权
衍生自丙型肝炎病毒的核酸和表达载体，转化细胞和丙型肝炎病毒颗粒各自通过使用它们制备
- 专利标题： Nucleic acid derived from hepatitis C virus and expression vector, transformed cell, and hepatitis C virus particles each prepared by using the same
- 专利标题（中）： 衍生自丙型肝炎病毒的核酸和表达载体，转化细胞和丙型肝炎病毒颗粒各自通过使用它们制备
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申请号： US13142227申请日： 2009-12-25
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公开(公告)号： US08834893B2公开(公告)日： 2014-09-16
- 发明人: Takaji Wakita , Tomoko Date , Hitoshi Takahashi
- 申请人： Takaji Wakita , Tomoko Date , Hitoshi Takahashi
- 申请人地址： JP Tokyo JP Tokyo JP Tokyo
- 专利权人： Toray Industries, Inc.,Japan as Represented by Director-General of National Institute of Infectious Diseases,Tokyo Metropolitan Institute of Medical Science
- 当前专利权人： Toray Industries, Inc.,Japan as Represented by Director-General of National Institute of Infectious Diseases,Tokyo Metropolitan Institute of Medical Science
- 当前专利权人地址： JP Tokyo JP Tokyo JP Tokyo
- 代理机构： Birch, Stewart, Kolasch & Birch, LLP
- 优先权： JP2008-335016 20081226
- 国际申请： PCT/JP2009/071644 WO 20091225
- 国际公布： WO2010/074249 WO 20100701
- 主分类号： C12N15/00
- IPC分类号： C12N15/00 ; C12N15/11 ; C12N15/33 ; C12N15/51 ; C12N7/00 ; C07K14/005 ; A61K39/00
摘要：
The present invention provides a nucleic acid comprises a 5′ untranslated region, an NS3 protein coding region, an NS4A protein coding region, an NS4B protein coding region, an NS5A protein coding region, an NS5B protein coding region, and a 3′ untranslated region of a hepatitis C virus genome, wherein the nucleic acid has nucleotide substitutions causing one or more amino acid substitutions selected from the group consisting of M(1205)K, F(1548)L, C(1615)W, T(1652)N, A(2196)T, A(2218)S, H(2223)Q, Q(2281)R, K(2520)N, and G(2374)S, as defined using the amino acid sequence shown in SEQ ID NO: 6 in the Sequence Listing as a reference sequence, in the NS3 protein coding region, the NS5A protein coding region, or the NS5B protein coding region.