The present invention provides an ambient light sensing module, which comprises a sawtooth signal generating circuit, an optical sensing unit, and a comparing unit. The sawtooth signal generating circuit produces a sawtooth signal. The optical sensing unit senses a light source and produces a light-sensing signal. The comparing unit produces a pulse-width modulation (PWM) signal related to the intensity of the light source according to the light-sensing signal and the sawtooth signal so that the PWM signal can be used as the control signal of the electronic device. The ambient light sensing module further comprises at least a fuse for determining a processing parameter. A signal processing unit processes the light-sensing signal according to the processing parameter for outputting a converting signal. The comparing unit compares the converting signal with the sawtooth signal for producing the PWM signal. An adjusting unit produces an adjusting signal according to the PWM signal and the light intensity or according to the converting signal and the light intensity for controlling a trimming unit to trim the fuse and thus modifying the processing parameter.