发明授权
- 专利标题: Using populations of beads for the fabrication of arrays on surfaces
- 专利标题(中): 使用珠子的种群在表面上制造阵列
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申请号: US14204329申请日: 2014-03-11
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公开(公告)号: US09079148B2公开(公告)日: 2015-07-14
- 发明人: Roberto Rigatti , Geoffrey Paul Smith , Jonathan Mark Boutell
- 申请人: Illumina Cambridge Limited
- 申请人地址: GB Nr Saffron Walden
- 专利权人: Illumina Cambridge Limited
- 当前专利权人: Illumina Cambridge Limited
- 当前专利权人地址: GB Nr Saffron Walden
- 代理机构: Illumina, Inc.
- 代理商 Brent C. Moore
- 主分类号: C12P19/34
- IPC分类号: C12P19/34 ; B01J19/00 ; C12Q1/68
摘要:
The present invention provides methods for creating an array of features on a surface based on content transferred from a plurality of beads to the surface. Nucleic acid content can be transferred using a method including the steps of (a) providing a surface having one or more primer oligonucleotides attached to the surface; (b) providing a pool of beads, wherein beads in the pool have a plurality of templates attached thereto, the plurality comprising multiple copies of a single nucleic acid template sequence; (c) arraying the beads onto the surface by hybridizing the templates to the primer oligonucleotides; and (d) extending the primers to produce copies of the templates attached to the surface.
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