发明授权
- 专利标题: Method for the separation of cells
- 专利标题(中): 细胞分离方法
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申请号: US12743395申请日: 2008-11-20
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公开(公告)号: US09133263B2公开(公告)日: 2015-09-15
- 发明人: Andreas Bosio
- 申请人: Andreas Bosio
- 申请人地址: DE Bergisch Gladbach
- 专利权人: MILTENYI BIOTEC GMBH
- 当前专利权人: MILTENYI BIOTEC GMBH
- 当前专利权人地址: DE Bergisch Gladbach
- 代理机构: Nixon Peabody LLP
- 代理商 Mark J. FitzGerald
- 优先权: EP07022478 20071120
- 国际申请: PCT/IB2008/003708 WO 20081120
- 国际公布: WO2009/066180 WO 20090528
- 主分类号: A01K67/00
- IPC分类号: A01K67/00 ; C12N15/00 ; C07K14/705 ; C12N5/0735 ; C12N5/00
摘要:
The invention relates to a cell containing a gene encoding a conditional transgenic surface marker that is detectable upon expression on the surface of the cell, wherein the gene encoding a conditional transgenic surface marker comprises: (i) a promoter, operably linked to (ii) a first transcription sequence, and (iii) a second transcription sequence encoding the surface marker, whereby the first transcription sequence prevents the transcription of the second transcription sequence, whereby the first transcription sequence is conditionally removable such that the second transcription sequence is transcribable, and whereby the surface marker renders the cell sortable through the detection of the conditional transgenic surface marker. Furthermore, the invention relates to a construct for generating such a cell, and to a method for separating such a cell from a population of cells.
公开/授权文献
- US20110004952A1 METHOD FOR THE SEPARATION OF CELLS 公开/授权日:2011-01-06
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