公开(公告)号：US07554657B2

公开(公告)日：2009-06-30

申请号：US11580378

申请日：2006-10-13

申请人： Andreas Bosio

发明人： Andreas Bosio

IPC分类号： G01N21/01

CPC分类号： G02B21/34 ,  B01L9/52 ,  B01L2200/025 ,  B01L2300/0822 ,  G01N35/00029

摘要： A receiving device for object holders comprises a base part. A plurality of centering elements are connected with the base part. The centering elements are arranged such that a plurality of receiving areas for receiving one object holder each are configured. The centering elements form a preferably circumferential centering frame around each receiving area.

摘要翻译： 用于对象夹持器的接收装置包括基座部分。 多个定心元件与基部连接。 定心元件被布置成使得构成用于接收一个物体保持器的多个接收区域。 定心元件在每个接收区域周围形成优选圆周定心框架。

公开(公告)号：US07378279B2

公开(公告)日：2008-05-27

申请号：US10472132

申请日：2002-03-15

申请人： Andreas Bosio

发明人： Andreas Bosio

IPC分类号： G01N1/31 ,  G01N1/00 ,  C12Q1/68

CPC分类号： G01N1/312 ,  Y10T436/113332 ,  Y10T436/25

摘要： An analyzing device for analyzing chemical and/or biological samples comprises a plurality of base parts, e. g. object holders, in a receiving device. The base parts are closed by a lid which is configured such that each base part and the lid form a test chamber for receiving the sample. Further, a conveying means for moving the sample in the test chamber is provided. The pumps of the conveying means are connected via channels with the test chamber and are controlled such that a portion of the sample liquid is alternately drawn off the test chamber and supplied back thereto by the pumps.

摘要翻译： 用于分析化学和/或生物样品的分析装置包括多个基部件， G。 对象持有人，在接收设备中。 基部由盖子封闭，盖子构造成使得每个基部和盖子形成用于接收样品的测试室。 此外，提供了用于使样本在测试室中移动的输送装置。 输送装置的泵通过通道与测试室连接并被控制，使得一部分样品液体被交替地从测试室中抽出并由泵反馈到其上。

公开(公告)号：US20070030480A1

公开(公告)日：2007-02-08

申请号：US11580378

申请日：2006-10-13

申请人： Andreas Bosio

发明人： Andreas Bosio

IPC分类号： G01N21/01

CPC分类号： G02B21/34 ,  B01L9/52 ,  B01L2200/025 ,  B01L2300/0822 ,  G01N35/00029

摘要： A receiving device for object holders comprises a base part. A plurality of centering elements are connected with the base part. The centering elements are arranged such that a plurality of receiving areas for receiving one object holder each are configured. The centering elements form a preferably circumferential centering frame around each receiving area.

摘要翻译： 用于对象夹持器的接收装置包括基座部分。 多个定心元件与基部连接。 定心元件被布置成使得构成用于接收一个物体保持器的多个接收区域。 定心元件在每个接收区域周围形成优选圆周定心框架。

公开(公告)号：US06830886B1

公开(公告)日：2004-12-14

申请号：US09701584

申请日：2001-02-01

申请人： Andreas Bosio ,  Wilhelm Stoffel ,  Markus Stoffel

发明人： Andreas Bosio ,  Wilhelm Stoffel ,  Markus Stoffel

IPC分类号： C12Q168

CPC分类号： C12Q1/6834 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00722 ,  C07B2200/11 ,  C07H21/00 ,  C40B40/06

摘要： The invention relates to a support. Oligonucleotides or polynucleotides are covalently bound with the 5′- or 3′-termination on least one main surface of said support via bifunctional spacers and bifunctional linkers. The support is characterized in that the oligonucleotides or polynucleotides which are covalently bound with the 5′- or 3′-termination via bifunctional spacers and bifunctional linkers comprise 200 to 600 bp, and the oligonucleotides or polynucleotides can be obtained by using a method which comprises the following steps: Selecting homologous regions of mRNA of a target species and of at least one model species; selecting amplification primers which permit the amplification of 200 to 600, preferably 200 to 400 bp long nucleic acids from the homologous regions of both the mRNA of the target species and the mRNA of at least one model species, whereby the amplification primers optionally comprise a maximum of 1 mismatch per 6 nucleic acids of the amplification primer; immobilizing the nucleic acids on at the least one main surface of the support, said nucleic acids being obtained from the corresponding 200 to 600 bp long nucleic acids which are amplified for the target species or for the at least one model species by amplifications using the amplification primers.

摘要翻译： 本发明涉及一种支撑体。 寡核苷酸或多核苷酸通过双官能间隔基和双官能连接体与所述载体的至少一个主表面上的5'或3'端基共价结合。 该载体的特征在于通过双功能间隔基和双官能连接基与5'-或3'末端共价结合的寡核苷酸或多核苷酸包含200至600bp，并且寡核苷酸或多核苷酸可以通过使用包含 以下步骤：选择目标物种和至少一种模式物种的mRNA的同源区; 选择允许从目标物种的mRNA的同源区和至少一个模型物种的mRNA的同源区扩增200至600个，优选200至400bp长的核酸的扩增引物，由此扩增引物任选地包含最大 每扩增引物6个核酸1个错配; 将核酸固定在载体的至少一个主表面上，所述核酸从相应的200至600bp长的核酸获得，所述核酸通过扩增用于靶物种或至少一种模型物种扩增 引物。

公开(公告)号：US09133263B2

公开(公告)日：2015-09-15

申请号：US12743395

申请日：2008-11-20

申请人： Andreas Bosio

发明人： Andreas Bosio

IPC分类号： A01K67/00 ,  C12N15/00 ,  C07K14/705 ,  C12N5/0735 ,  C12N5/00

CPC分类号： C07K14/705 ,  C07K14/70589 ,  C07K14/70596 ,  C12N5/0606 ,  C12N2510/00

摘要： The invention relates to a cell containing a gene encoding a conditional transgenic surface marker that is detectable upon expression on the surface of the cell, wherein the gene encoding a conditional transgenic surface marker comprises: (i) a promoter, operably linked to (ii) a first transcription sequence, and (iii) a second transcription sequence encoding the surface marker, whereby the first transcription sequence prevents the transcription of the second transcription sequence, whereby the first transcription sequence is conditionally removable such that the second transcription sequence is transcribable, and whereby the surface marker renders the cell sortable through the detection of the conditional transgenic surface marker. Furthermore, the invention relates to a construct for generating such a cell, and to a method for separating such a cell from a population of cells.

摘要翻译： 本发明涉及包含编码条件转基因表面标记的基因的细胞，其在细胞表面上表达时可检测，其中编码有条件的转基因表面标志的基因包括：（i）与（ii）有效连接的启动子， 第一转录序列，和（iii）编码表面标记的第二转录序列，其中第一转录序列阻止第二转录序列的转录，由此第一转录序列有条件地可除去，使得第二转录序列是可转录的，以及 由此表面标记通过检测条件转基因表面标记使得细胞可分类。 此外，本发明涉及用于产生这种细胞的构建体，以及用于从细胞群分离这样的细胞的方法。

公开(公告)号：US20110004952A1

公开(公告)日：2011-01-06

申请号：US12743395

申请日：2008-11-20

申请人： Andreas Bosio

发明人： Andreas Bosio

IPC分类号： A01K67/00 ,  C12N15/63 ,  C12N15/00 ,  C12N5/071 ,  C07K14/00 ,  C07K16/00 ,  C12N5/073

CPC分类号： C07K14/705 ,  C07K14/70589 ,  C07K14/70596 ,  C12N5/0606 ,  C12N2510/00

摘要： The invention relates to a cell containing a gene encoding a conditional transgenic surface marker that is detectable upon expression on the surface of the cell, wherein the gene encoding a conditional transgenic surface marker comprises: (i) a promoter, operably linked to (ii) a first transcription sequence, and (iii) a second transcription sequence encoding the surface marker, whereby the first transcription sequence prevents the transcription of the second transcription sequence, whereby the first transcription sequence is conditionally removable such that the second transcription sequence is transcribable, and whereby the surface marker renders the cell sortable through the detection of the conditional transgenic surface marker. Furthermore, the invention relates to a construct for generating such a cell, and to a method for separating such a cell from a population of cells.

摘要翻译： 本发明涉及包含编码条件转基因表面标记的基因的细胞，其在细胞表面上表达时可检测，其中编码有条件的转基因表面标志的基因包括：（i）与（ii）有效连接的启动子， 第一转录序列，和（iii）编码表面标记的第二转录序列，其中第一转录序列阻止第二转录序列的转录，由此第一转录序列有条件地可除去，使得第二转录序列是可转录的，以及 由此表面标记通过检测条件转基因表面标记使得细胞可分类。 此外，本发明涉及用于产生这种细胞的构建体，以及用于从细胞群分离这样的细胞的方法。

公开(公告)号：US20090155126A1

公开(公告)日：2009-06-18

申请号：US11920197

申请日：2006-05-18

申请人： Stefan Miltenyi ,  Juergen Schulz ,  Elmar Niklas Neuschaefer ,  Andreas Bosio ,  Jan Drewes

发明人： Stefan Miltenyi ,  Juergen Schulz ,  Elmar Niklas Neuschaefer ,  Andreas Bosio ,  Jan Drewes

IPC分类号： B01L3/00

CPC分类号： B01L7/52 ,  B01L3/5025 ,  B01L3/5027 ,  B01L2300/0822 ,  B01L2300/0877 ,  B01L2400/0487 ,  G01N1/312

摘要： A device for carrying out chemical and/or biological reactions comprises a sample chamber (38) formed by a first portion (12) and a second portion (26) and designed for holding the sample. With the aid of a moving means (46,50) the sample can be moved in the sample chamber (38). The second portion (26) is connected via an intermediate element (27) with a heating and cooling-control element (29) for heating and cooling the sample. For ensuring good temperature conductivity and resistance to the sample of the second portion (26), in a preferred embodiment, the second portion (26) is made of ceramic.

摘要翻译： 用于进行化学和/或生物反应的装置包括由第一部分（12）和第二部分（26）形成并被设计用于保持样品的样品室（38）。 借助于移动装置（46,50），样品可以在样品室（38）中移动。 第二部分（26）经由中间元件（27）与加热和冷却控制元件（29）连接，用于加热和冷却样品。 为了确保良好的温度传导性和对第二部分（26）的样品的耐受性，在优选实施例中，第二部分（26）由陶瓷制成。

公开(公告)号：US20080009614A1

公开(公告)日：2008-01-10

申请号：US11600326

申请日：2006-11-16

申请人： Andreas Bosio

发明人： Andreas Bosio

IPC分类号： C07H21/04

CPC分类号： C12N15/1013 ,  C12N15/1096

摘要： A nucleic acid complex with an anchor oligonucleotide, an arbitrary nucleic acid or an arbitrary oligonucleotide and a linker nucleic acid, wherein the linker nucleic acid is partially hybridized with the anchor nucleotide and partially hybridized with the arbitrary nucleic acid or the arbitrary oligonucleotide and the anchor nucleotide is immobilized on a support by its 3′-terminus.

摘要翻译： 与锚定寡核苷酸，任意核酸或任意寡核苷酸和连接体核酸的核酸复合物，其中连接体核酸与锚定核苷酸部分杂交并与任意核酸或任意寡核苷酸和锚定部分杂交 核苷酸通过其3'末端固定在载体上。