发明授权
US09464281B2 Method for concentrating viruses, method for concentrating cells or bacteria, and magnetic composite
有权
浓缩病毒的方法,浓缩细胞或细菌的方法以及磁性复合材料
- 专利标题: Method for concentrating viruses, method for concentrating cells or bacteria, and magnetic composite
- 专利标题(中): 浓缩病毒的方法,浓缩细胞或细菌的方法以及磁性复合材料
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申请号: US12844742申请日: 2010-07-27
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公开(公告)号: US09464281B2公开(公告)日: 2016-10-11
- 发明人: Yasuo Suda , Masahiro Wakao , Takashi Kodama
- 申请人: Yasuo Suda , Masahiro Wakao , Takashi Kodama
- 申请人地址: JP Kagoshima-shi JP Kagoshima-shi JP Osaka-shi
- 专利权人: SUDx-Biotec Corporation,National University Corporation Kagoshima University,Neat Co., Ltd.
- 当前专利权人: SUDx-Biotec Corporation,National University Corporation Kagoshima University,Neat Co., Ltd.
- 当前专利权人地址: JP Kagoshima-shi JP Kagoshima-shi JP Osaka-shi
- 代理机构: Morrison & Foerster LLP
- 优先权: JP2009-175001 20090728; JP2010-045753 20100302
- 主分类号: C12N7/02
- IPC分类号: C12N7/02 ; G01N33/566 ; C12N13/00 ; B03C1/01 ; B03C1/28 ; B82Y25/00 ; C12N1/20 ; C12N7/00 ; C12Q1/24 ; G01N33/543 ; H01F1/00
摘要:
A method for concentrating viruses includes applying a magnetic force to a mixture containing: sugar chain-immobilized magnetic metal nano-particles each having a structure in which a sugar chain-immobilized metal nano-particle is bound to a first magnetic nano-particle; second magnetic particles with mean particle size larger than that of the sugar chain-immobilized magnetic metal nano-particles; and a specimen. Each sugar chain-immobilized metal nano-particle has a structure where a ligand-conjugate is bound to a metal nano-particle via sulfur atoms. The ligand-conjugate has a structure where a linker compound's amino group is connected to a sugar chain having a reducing terminal. The linker compound includes, in molecules thereof, an amino group, sulfur atoms, and a hydrocarbon chain having carbon-nitrogen bonds. This allows short-time concentration of viruses in a sufficient amount almost equal to that of centrifugation concentration, allowing safely and effectively concentrating target viruses, resulting in prompt, easy, and highly sensitive detection and identification of viruses.
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