Invention Grant
US09522218B2 Method for preparing porous scaffold for tissue engineering, cell culture and cell delivery
有权
用于组织工程,细胞培养和细胞递送的多孔支架的制备方法
- Patent Title: Method for preparing porous scaffold for tissue engineering, cell culture and cell delivery
- Patent Title (中): 用于组织工程,细胞培养和细胞递送的多孔支架的制备方法
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Application No.: US12681682Application Date: 2008-10-10
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Publication No.: US09522218B2Publication Date: 2016-12-20
- Inventor: Catherine Le Visage , Didier Letourneur
- Applicant: Catherine Le Visage , Didier Letourneur
- Applicant Address: FR Paris FR Paris
- Assignee: INSERM (Institut National de la Santé et de la Recherche Médicale),Université Paris 7—Denis Diderot
- Current Assignee: INSERM (Institut National de la Santé et de la Recherche Médicale),Université Paris 7—Denis Diderot
- Current Assignee Address: FR Paris FR Paris
- Agency: McAndrews, Held & Malloy, Ltd.
- Priority: EP07301452 20071011
- International Application: PCT/EP2008/063671 WO 20081010
- International Announcement: WO2009/047346 WO 20090416
- Main IPC: A61L27/20
- IPC: A61L27/20 ; C08J3/075 ; C08B37/00 ; C08B37/02 ; C08B37/04 ; C08B37/10 ; C08B37/12 ; C08J9/08 ; A61L27/52 ; A61L27/56 ; A61L27/38 ; A61L27/50 ; C12N5/00 ; C08B37/08 ; C08J9/28

Abstract:
The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprises the steps consisting of: a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide, an amount of a cross-linking agent and an amount of a porogen agent b) transforming the solution into a hydrogel by placing said solution at a temperature from about 4° C. to about 80° C. for a sufficient time to allow the cross-linking of said amount of polysaccharide and c) submerging said hydrogel into an aqueous solution d) washing the porous scaffold obtained at step c).
Public/Granted literature
- US20100221303A1 Method for Preparing Porous Scaffold for Tissue Engineering, Cell Culture and Cell Delivery Public/Granted day:2010-09-02
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