- 专利标题: Production of recombinant proteins with simple glycoforms
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申请号: US15171977申请日: 2016-06-02
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公开(公告)号: US09670271B2公开(公告)日: 2017-06-06
- 发明人: Nan Lin , Natalie Sealover , Henry George , Kevin Kayser
- 申请人: SIGMA-ALDRICH CO. LLC
- 申请人地址: US MO St. Louis
- 专利权人: SIGMA-ALDRICH CO. LLC
- 当前专利权人: SIGMA-ALDRICH CO. LLC
- 当前专利权人地址: US MO St. Louis
- 代理机构: Polsinelli PC
- 主分类号: C12P21/02
- IPC分类号: C12P21/02 ; C07K16/10 ; C12P21/00 ; C12N9/10 ; C07K16/00 ; C12P21/04 ; C12N15/63 ; C12N15/67 ; C12N15/85
摘要:
The present invention provides cell lines deficient in mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I (Mgat1). Also provided are methods for producing the Mga1 deficient cell lines and methods for using the Mgat1 deficient cell lines for the production of recombinant proteins having simple glycoforms.
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