Invention Grant
- Patent Title: Digital counting of individual molecules by stochastic attachment of diverse labels
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Application No.: US14540029Application Date: 2014-11-12
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Publication No.: US09708659B2Publication Date: 2017-07-18
- Inventor: Stephen P. A. Fodor , Glenn K. Fu
- Applicant: Cellular Research, Inc.
- Applicant Address: US CA Menlo Park
- Assignee: Cellular Research, Inc.
- Current Assignee: Cellular Research, Inc.
- Current Assignee Address: US CA Menlo Park
- Agency: Knobbe, Martens, Olson & Bear, LLP
- Main IPC: C40B60/08
- IPC: C40B60/08 ; G01N33/48 ; C40B20/04 ; C40B30/04 ; C12Q1/68 ; C12N15/10 ; G06F19/20 ; G06F19/22

Abstract:
Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.
Public/Granted literature
- US20150099673A1 DIGITAL COUNTING OF INDIVIDUAL MOLECULES BY STOCHASTIC ATTACHMENT OF DIVERSE LABELS Public/Granted day:2015-04-09
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