A voltage-controlled oscillator gain measurement system includes a voltage-controlled oscillator, a voltage detector, and a processor. The voltage-controlled oscillator, which is configured in a phase-locked loop circuit, generates an output signal with an output frequency according to a control signal. The control signal is generated according to the output signal divided by a scaling number. The voltage detector is configured to measure a voltage difference of the control signal. The processor adjusts the scaling number to generate an output frequency difference of the output signal, and obtains a reciprocal gain of the voltage-controlled oscillator by dividing the voltage difference by the output frequency difference.