- 专利标题: Counting particles using an electrical differential counter
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申请号: US13884580申请日: 2011-11-09
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公开(公告)号: US09976973B2公开(公告)日: 2018-05-22
- 发明人: Nicholas Watkins , Rashid Bashir , William Rodriguez , Xuanhong Cheng , Mehmet Toner , Grace Chen , Aaron Oppenheimer
- 申请人: Nicholas Watkins , Rashid Bashir , William Rodriguez , Xuanhong Cheng , Mehmet Toner , Grace Chen , Aaron Oppenheimer
- 申请人地址: US MA Boston US MA Cambridge US IL Urbana US MA Cambridge
- 专利权人: The General Hospital Corporation,Massachusetts Institute of Technology,The Board of Trustees of the University of Illinois,Daktari Diagnostics, Inc.
- 当前专利权人: The General Hospital Corporation,Massachusetts Institute of Technology,The Board of Trustees of the University of Illinois,Daktari Diagnostics, Inc.
- 当前专利权人地址: US MA Boston US MA Cambridge US IL Urbana US MA Cambridge
- 代理机构: Fish & Richardson P.C.
- 国际申请: PCT/US2011/060041 WO 20111109
- 国际公布: WO2012/064878 WO 20120518
- 主分类号: G01N15/10
- IPC分类号: G01N15/10 ; G01N27/02 ; G01N15/12 ; C12M1/34 ; G01N33/569 ; G01N15/14
摘要:
This disclosure relates to methods and devices to count particles of interest, such as cells. The methods include obtaining a fluid sample that may contain particles of interest; counting all types of particles in a portion of the sample using a first electrical differential counter to generate a first total; removing any particles of interest from the portion of the fluid sample; counting any particles remaining in the portion of the fluid sample using a second electrical differential counter after the particles of interest are removed to generate a second total; and calculating a number of particles of interest originally in the fluid sample by subtracting the second total from the first total, wherein the difference is the number of particles of interest in the sample. These methods and related devices can be used, for example, to produce a robust, inexpensive diagnostic kit for CD4+ T cell counting in whole blood samples.