Methods and devices for evaluating a sample, e.g., a plasma sample, from a subject for detecting a target red blood cell protein or antibody are disclosed. Antibody screening methods and devices significantly reduce the level of non-specific binding to a surface (e.g., a test surface bound with a red blood cell (rbcm) preparation), allowing for more efficient detection and reduced test time. The antibody screening method includes an immunoglobulin G (IgG) binding moiety that binds selectively and specifically to the plasma IgG present relative to the binding to the lysed rbcm preparation. An antibody screening method is disclosed whereby non-specific binding caused by lysed red blood cell membrane preparations can be reduced by an agent that specifically cleaves a human IgG in the hinge region. Additional methods and devices for target capturing include a substantially planar surface having an optimized angle for capture, or alternative solid phase geometries for capture.