发明申请
- 专利标题: METHOD OF GENERATING A LIBRARY OF POLYNUCLEOTIDE MOLECULES ENCODING GUIDE RNAS
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申请号: PCT/GB2022/052509申请日: 2022-10-04
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公开(公告)号: WO2023057746A1公开(公告)日: 2023-04-13
- 发明人: WU, Qianxin , TREHAN, Ashutosh
- 申请人: GENOME RESEARCH LIMITED
- 申请人地址: Wellcome Trust Genome Campus
- 专利权人: GENOME RESEARCH LIMITED
- 当前专利权人: GENOME RESEARCH LIMITED
- 当前专利权人地址: Wellcome Trust Genome Campus
- 代理机构: BELL, Lewis et al.
- 优先权: GB2114206.2 2021-10-04
- 主分类号: C12N9/22
- IPC分类号: C12N9/22 ; C12N15/113 ; C12Q1/6806 ; C12N15/10 ; C12N15/1093 ; C12N15/11 ; C12N2310/20 ; C12N2310/345 ; C12N2330/31 ; C12Y301/21004
摘要:
The invention relates to a method of generating a library of polynucleotide molecules encoding guide RNAs (gRNAs) from target polynucleotide(s). The invention also relates to a library of polynucleotide molecules encoding gRNAs obtainable by the aforementioned method, and a gRNA library generation kit thereof.