公开(公告)号：WO2023089180A1

公开(公告)日：2023-05-25

申请号：PCT/EP2022/082644

申请日：2022-11-21

Applicant: IST INNUSCREEN GMBH

Inventor： HILLEBRAND, Timo

IPC: C12Q1/6806 ,  G01N1/40

Abstract: Die Erfindung betrifft ein Verfahren und Testkit zur preiswerten und ressourcensparenden Extraktion von Nukleinsäuren, wobei unter „ressourcensparend" die weitgehende Vermeidung von Plastik-Müll zu verstehen ist. Das Verfahren umfasst folgende Schritte: Zugabe eines Bindungspuffers zu einer Nukleinsäure enthaltenden Lösung, wobei die Nukleinsäuren an eine feste Phase angebunden werden, Waschen der angebundenen Nukleinsäuren, Eluieren der gewaschenen Nukleinsäuren, dadurch gekennzeichnet, dass als feste Phase ein Reaktionsgefäß dient, welche an seiner Innenseite Rillen oder einen Gewindeschnitt aufweist, woran die Anbindung der Nukleinsäuren erfolgt. Vor den Anbinden der Nukleinsäuren können diese mittels einer Lyse aus Zellen freigesetzt werden. Das Testkit umfasst mindestens ein Reaktionsgefäß, das an seiner Innenseite Rillen oder einen Gewindeschnitt aufweist, mindestens einen Bindungspuffer zum Anbinden der Nukleinsäuren an die Innenseite des Reaktionsgefäßes, mindestens einen Waschpuffer zum Waschen der angebundenen Nukleinsäuren, mindestens einen Elutionspuffer zum Eluieren der gewaschenen Nukleinsäuren und ggf. Lysepuffer.

公开(公告)号：WO2023087171A1

公开(公告)日：2023-05-25

申请号：PCT/CN2021/131151

申请日：2021-11-17

Applicant: 王锦弘

Inventor： 王锦弘 WANG, Chin Hung

IPC: C12Q1/6806 ,  G01N1/00 ,  C12M1/00 ,  B01L3/00 ,  A61B10/02

Abstract: 一种采检棒容置构件（1），包含第一开口（11）及第二开口（12），第一开口（11）与第二开口（12）相连，且相连处包含窄口（13），且采检棒容置构件（1）在z轴方向具有第一厚度；其中当采检棒（200）置入设置在离心管（100）中的所述采检棒容置构件（1）的所述第一开口（11）且离心时，所述采检棒（200）自所述第一开口（11）通过所述窄口（13）进入所述第二开口（12）。

公开(公告)号：WO2023086942A2

公开(公告)日：2023-05-19

申请号：PCT/US2022/079721

申请日：2022-11-11

Applicant: THE FLORIDA STATE UNIVERSITY RESEARCH FOUNDATION, INC.

Inventor： SINGH, Prashant ,  BOSILEVAC, Joseph, M.

IPC: C12Q1/689 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6853

Abstract: Knowing a target organism's concentration range in the biological sample is critical for many industries, including the food and diagnostic industries. For example, foodborne pathogens (i.e., Salmonella, Campylobacter), which are not considered an adulterant in food, can be present in food samples at varying concentrations. Disclosed herein are methods and kits for quantifying the presence of a microorganism in a sample. Specifically, disclosed are methods for quantifying a pathogen in a sample, such as a food sample, to determine if the levels of pathogen present in the sample are within an acceptable range.

公开(公告)号：WO2023085955A1

公开(公告)日：2023-05-19

申请号：PCT/NZ2022/050140

申请日：2022-11-09

Applicant: VICTORIA LINK LIMITED ,  WAIKATOLINK LIMITED

Inventor： PATRICK, Wayne ,  OULAVALLICKAL, Tifany ,  ARCUS, Vickery Laurence ,  HICKS, Joanna

IPC: C12N15/10 ,  C12N15/70 ,  C12N9/00 ,  C12Q1/25 ,  C12Q1/6806

Abstract: The present disclosure encompasses RNA ligase enzymes, particularly thermostable RNA ligase enzymes, and including variant enzymes, along with the preparation and use thereof. Specifically encompassed are methods for preparing the RNA ligase enzymes, methods for ligation using these enzymes, as well as polynucleotides used for producing these enzymes, libraries comprising these polynucleotides, and microbial strains comprising these polynucleotides.

公开(公告)号：WO2023085117A1

公开(公告)日：2023-05-19

申请号：PCT/JP2022/040284

申请日：2022-10-28

Applicant: 国立大学法人広島大学

Inventor： 岡村　好子 ,  高橋　宏和

IPC: C12N15/09 ,  C12Q1/6806

Abstract: 鋳型ＤＮＡの製造方法は、カセット２本鎖ＤＮＡを準備する工程（ａ）と、標的配列含有オリゴヌクレオチドを調製する工程（ｂ）と、それらをＤＮＡ結合酵素により連結し、標的配列含有環状体ヌクレオチドを得る工程（ｃ）と、該ヌクレオチドを鋳型として鎖置換型ＤＮＡポリメラーゼにより増幅し、サポート配列と、ＲＮＡポリメラーゼのプロモーター配列と、標的配列と、ｓｇＲＮＡスキャフォールド配列とからなる反復配列を有する同一配列コンカテマーとなる標的配列含有２本鎖ＤＮＡを得る工程（ｄ）と、該２本鎖ＤＮＡを同一配列同士の間で切断し、サポート配列とＲＮＡポリメラーゼのプロモーター配列と標的配列とｓｇＲＮＡスキャフォールド配列とからなる配列を有する鋳型ＤＮＡを得る工程（ｅ）とを備えている。

公开(公告)号：WO2023084369A1

公开(公告)日：2023-05-19

申请号：PCT/IB2022/060640

申请日：2022-11-04

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： WATTS, Annabelle ,  BONILLA, Tonya D. ,  RASMUSSEN, Jerald K. ,  FISHMAN, Joshua M. ,  TRAEGER, Lindsay L. ,  COLAK ATAN, Semra

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6813

Abstract: A method of processing polynucleic acids is described comprising: providing a solid support comprising ligands with an amide group; exposing the solid support to polynucleic acid molecules in a buffer having a pH less than 5.5 to bind at least a portion of the polynucleic acid molecules to the ligands; c) exposing the solid support (e.g. particles) with bound polynucleic acid molecules to a buffer having a pH greater than 6 to release a portion of the bound polynucleic acid molecules from the ligands of the particles and to retain a portion of the polynucleic acid molecules bound to the solid support (e.g. particles); and utilizing the solid support with retained bound polynucleic acid molecules or suspension thereof.

公开(公告)号：WO2023081796A1

公开(公告)日：2023-05-11

申请号：PCT/US2022/079270

申请日：2022-11-04

Applicant: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH

Inventor： TAYLOR, William R. ,  KISIEL, John B. ,  MAHONEY, Douglas W.

IPC: C12Q1/6886 ,  C12N15/10 ,  C12Q1/6806

Abstract: The present disclosure relates to detecting one or more types of oropharyngeal cancer in a biological sample from a subject. In particular, the present disclosure provides compositions and methods for detecting the presence or absence of one or more types of oropharyngeal cancer (e.g., HPV+ oropharyngeal squamous cell cancer) in a biological sample from a subject having or suspected of having an oropharyngeal cancer.

公开(公告)号：WO2023079140A1

公开(公告)日：2023-05-11

申请号：PCT/EP2022/080978

申请日：2022-11-07

Applicant: BLINK AG

Inventor： ERMANTRAUT, Eugen ,  ELLINGER, Thomas ,  LEMUTH, Oliver ,  STEINMETZER, Katrin ,  KLINGNER, Susanne ,  KANITZ, Lea

IPC: C12Q1/6806 ,  C12Q1/686

Abstract: The present invention relates to microsphere comprising a first material, preferably a first polymer, and a second material, preferably a second polymer. The present invention also relates to a liquid phase comprising a plurality of such microspheres. Furthermore, the present invention relates to a method for capturing a nucleic acid from a sample and to a method of amplifying a nucleic acid from a sample. The present invention also relates to the use of a plurality of microspheres in an assay for the detection of multiple nucleic acid analytes. Furthermore, the present invention relates to the use of a plurality of microspheres in an assay for the detection of a single nucleic acid analyte in multiple samples.

公开(公告)号：WO2023073635A1

公开(公告)日：2023-05-04

申请号：PCT/IB2022/060393

申请日：2022-10-28

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： LAUBER, Matthew A. ,  NGUYEN, Jennifer M. ,  LIU, Xiaoxiao ,  DONEGAN, Michael

IPC: C12Q1/6806

Abstract: The present disclosure provides compositions and methods for sample processing, particularly for oligonucleotide analysis e.g. analysis of formulated nucleic acid drugs. A composition for pretreating at least one target nucleic acid in a biological mixture provided herein includes a chaotropic agent selected from a substituted guanidine, a substituted amidine, a substituted quaternary amine, or a combination thereof, an optional protease, and/or an optional disulfide-reducing agent. Methods of analyzing at least one target nucleic acid in a biological mixture is also provided herein. Furthermore, the present disclosure provides methods for quantifying at least one target cationic lipid interacting with a nucleic acid.

公开(公告)号：WO2023069367A1

公开(公告)日：2023-04-27

申请号：PCT/US2022/046906

申请日：2022-10-17

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： LACKEY, Jeremy ,  BANYAI, William

IPC: C12Q1/6869 ,  C12M1/34 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6876 ,  C12Q1/6806

Abstract: Provided herein are compositions, devices, systems and methods for polynucleotide sequencing. Further provided are devices comprising surfaces for continuous sequencing. The compositions, devices, systems and methods described herein provide improved storage, density, and retrieval of biomolecule-based information.