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公开(公告)号：EP1931772A4
公开(公告)日：2009-12-30
申请号：EP06801382
申请日：2006-08-10
发明人： HOGREFE HOLLY , AREZI BAHRAM , XING WEIMEI
CPC分类号： C12Y207/07049 , C12N9/1276
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公开(公告)号：EP1766065A4
公开(公告)日：2009-08-05
申请号：EP05755004
申请日：2005-05-25
CPC分类号： B82Y5/00 , B82Y10/00 , C12N9/1252 , C12N9/1276 , C12N15/1096
摘要： The present invention relates to composition, kits and methods comprising a mutant DNA 5 polymerase exhibiting increased reverse transcriptase activity. The invention also relates to methods of generating modified cDNA.
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公开(公告)号：EP1856294A4
公开(公告)日：2009-04-01
申请号：EP06748194
申请日：2006-02-09
发明人： HAPPE SCOTT , SORGE JOSEPH A , FIRMIN ANDREW
CPC分类号： C12Q1/6818 , C12Q2525/301
摘要： The invention relates to compositions and methods for detection of nucleic acid sequences. The invention further relates to kit format of said compositions for detection of nucleic acid sequences. Oligonucleotide probes of the invention comprise a target binding sequence and a sequence at least partially complementary thereto, joined by an optional linker to form a hairpin structure in the absence of the target nucleic acid sequence. The probes of the invention comprise a pair of moieties that produce a detectable signal when the probe hybridizes to the target sequence.
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公开(公告)号：EP1606389B1
公开(公告)日：2008-11-12
申请号：EP04718119.3
申请日：2004-03-05
发明人： SORGE, Joseph, A. , FIRMIN, Andrew
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , B82Y5/00 , B82Y10/00 , C12Q1/6827 , C12Q2565/1015 , C12Q2535/125
摘要： The present invention provides compositions, kits, and methods for detecting polynucleotide sequence differences. The method involves amplifying a polynucleotide in the presence of a labeled nucleotide whose incorporation into the amplified product can indicate the presence of a sequence difference within the polynucleotide template. The invention is particularly useful for differentiating two or more closely related polynucleotide sequences, for example, in determining which allele or alleles of a multiallelic organism are present in a target polynucleotide.
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公开(公告)号：EP1856294A2
公开(公告)日：2007-11-21
申请号：EP06748194.5
申请日：2006-02-09
发明人： HAPPE, Scott , SORGE, Joseph, A. , FIRMIN, Andrew
CPC分类号： C12Q1/6818 , C12Q2525/301
摘要： The invention relates to compositions and methods for detection of nucleic acid sequences. The invention further relates to kit format of said compositions for detection of nucleic acid sequences. Oligonucleotide probes of the invention comprise a target binding sequence and a sequence at least partially complementary thereto, joined by an optional linker to form a hairpin structure in the absence of the target nucleic acid sequence. The probes of the invention comprise a pair of moieties that produce a detectable signal when the probe hybridizes to the target sequence.
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公开(公告)号：EP1616033A4
公开(公告)日：2007-05-09
申请号：EP04758226
申请日：2004-03-19
发明人： BORNS MICHAEL
CPC分类号： C12N15/102 , C12N9/1252 , C12Q1/6844 , C12Q1/686 , C12Q2527/119 , C12Q2522/101 , C12Q2521/101
摘要： The present invention discloses methods of using DNA polymerase fusions at high pH in PCR, DNA sequencing and mutagenesis protocols.
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公开(公告)号：EP1423534A4
公开(公告)日：2006-08-30
申请号：EP02757178
申请日：2002-08-16
CPC分类号： C12Q1/6837 , C12Q1/6848 , C12Q2545/101
摘要： The present invention relates, in part, to control nucleic acid molecules having no significant sequence homology to any known nucleic acid, and predefined G/C-content. The present invention further relates to method of using control nucleic acid molecules to validate microarray analyses, compositions comprising control nucleic acid molecules, and kits comprising control nucleic acid molecules.
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9.发明授权
公开(公告)号：EP1373572B1
公开(公告)日：2006-07-26
申请号：EP02725061.2
申请日：2002-03-01
CPC分类号： C07F15/0093
摘要： The invention relates to novel platinum-based compounds for labeling biomolecules. Platinum based labeling compounds according to the invention irreversibly attach to a target biomolecule via coordination of a platinum (II) metal center with N or S atoms on the target biomolecule. The invention relates to the novel compounds themselves, methods of making the platinum-based labeling compounds, probes labeled with such compounds, methods of making such labeled probes, and kits comprising the novel platinum-based labeling compounds and/or probes labeled with them. The invention also relates to methods of using probes labeled with platinum-based labeling compounds of the invention, particularly array and microarray hybridization methods.
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公开(公告)号：EP0733098B1
公开(公告)日：2006-01-04
申请号：EP94931420.7
申请日：1994-10-20
申请人： STRATAGENE
CPC分类号： B01L7/52 , B01L7/525 , B01L7/5255 , B01L7/54 , B01L2200/147 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2300/1844 , G01N35/0099 , G01N35/028 , G01N2035/042 , Y10S435/809 , Y10T436/25
摘要： A method in which a temperature is generated across a gradient block, and an apparatus comprising a block (2, 17, 18, 19) across which a temperature gradient can be generated. By setting up such a gradient, multiple reaction mixtures held in wells (3) on the gradient block (2, 17, 18, 19) can be simultaneously run at temperatures which differ only slightly, thereby permitting an optimum temperature for the reaction to be quickly identified. In a preferred embodiment, the gradient block (2, 17, 18, 19) is integrated into a thermal cycler used for nucleic acid amplification reactions.
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