公开(公告)号：EP2318839A1

公开(公告)日：2011-05-11

申请号：EP09812131.2

申请日：2009-09-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： ROWELL, Craig ,  PANATTONI, Cory ,  BERKELMAN, Thomas, R. ,  CATER, Sean ,  PETERSEN, Shane ,  OLECH, Lee ,  YANG, Xuemei

IPC: G01N33/558 ,  G01N33/559 ,  G01N33/561

CPC classification number: G01N27/44747 ,  B01D57/02 ,  C08F220/56 ,  C08F222/385

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

Abstract translation: 聚丙烯酰胺凝胶在蛋白质分离中提供高分辨率并且相对于水解比常规聚丙烯酰胺凝胶更稳定，其依赖于Tris或Tris-Bis作为缓冲剂，通过掺入三乙醇胺代替大部分或全部的Tris或Tris-Bis制备。

公开(公告)号：EP2318839B1

公开(公告)日：2017-05-17

申请号：EP09812131.2

申请日：2009-09-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： ROWELL, Craig ,  PANATTONI, Cory ,  BERKELMAN, Thomas, R. ,  CATER, Sean ,  PETERSEN, Shane ,  OLECH, Lee ,  YANG, Xuemei

IPC: G01N33/558 ,  G01N33/559 ,  G01N33/561 ,  G01N27/447 ,  B01D57/02

CPC classification number: G01N27/44747 ,  B01D57/02 ,  C08F220/56 ,  C08F222/385

公开(公告)号：EP2364318A1

公开(公告)日：2011-09-14

申请号：EP09830962.8

申请日：2009-12-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： BERKELMAN, Thomas, R.

IPC: C07F5/00 ,  C07F11/00 ,  C07F13/00 ,  C07F15/00

CPC classification number: C07F15/0033 ,  C09B57/00 ,  C09B57/008 ,  C09B57/10 ,  G01N33/52 ,  G01N33/6803

Abstract: A complex containing a transition metal ion and a plurality of donor ligands each of which is fully coordinated to the transition metal ion and is either a nitrogen donor ligand or a cyclometalated donor ligand, such that at least one of the donor ligands is a cyclometalated donor ligand bears one or more reactive groups connected to at least one of the donor ligands through a linker that includes a chain of four or more atoms. The linker offers advantages that make the complex particularly effective in labeling biomolecules and in multiplex analyses.

公开(公告)号：EP2547666B1

公开(公告)日：2015-11-04

申请号：EP11756750.3

申请日：2011-03-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： BERKELMAN, Thomas, R. ,  MARTIN, Lisandra ,  JONES, Rhiannon ,  WALKER II, John

IPC: C07D255/02 ,  B01D15/00 ,  G01N30/00 ,  G01N33/00

CPC classification number: G01N27/44747 ,  B01D15/3823 ,  C07D255/02 ,  C07F3/06 ,  C07F13/005 ,  C08F220/56 ,  C08F220/60 ,  G01N33/54306 ,  Y10T436/143333 ,  Y10T436/163333

公开(公告)号：EP2547666A1

公开(公告)日：2013-01-23

申请号：EP11756750.3

申请日：2011-03-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： BERKELMAN, Thomas, R. ,  MARTIN, Lisandra ,  JONES, Rhiannon ,  WALKER II, John

IPC: C07D255/02

CPC classification number: G01N27/44747 ,  B01D15/3823 ,  C07D255/02 ,  C07F3/06 ,  C07F13/005 ,  C08F220/56 ,  C08F220/60 ,  G01N33/54306 ,  Y10T436/143333 ,  Y10T436/163333

Abstract: Conjugates of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes with a variety of conjugating members are used in the formation of dinuclear metal complexes which bind to phosphate esters. By virtue of their conjugated forms, the complexes are incorporated into chromatographic media, affinity binding reagents, and dyes, which make the complexes useful in a wide range of assays, separations, and purifications. In addition, dinuclear metal complexes of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes that are not so conjugated are used in the detection of phosphate esters of biological species by either MALDI-TOF mass spectrometry or by dye displacement.

公开(公告)号：EP2057508A1

公开(公告)日：2009-05-13

申请号：EP07814469.8

申请日：2007-08-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： BERKELMAN, Thomas, R. ,  YARMOLUK, Sergiy, M. ,  KOVALSKA, Vladyslava, B. ,  LOSYTSKYY, Mykhaylo, Yu. ,  VOLKOVA, Kateryna, D.

IPC: G03F9/00

CPC classification number: G01N33/6839 ,  C09B23/0066 ,  C09B57/007 ,  G01N2458/30

Abstract: Squaraine dyes are incorporated into a separation medium in which protein or polypeptide mixtures are separated, which medium also contains a detergent that forms a complex with the polypeptides or proteins. The dyes, upon excitation in the separation medium, exhibit a heretofore unrecognized selectivity in their fluorescent emissions by emitting a signal only when the dye molecules are associated with complexes of protein (or polypeptide) and detergent molecules, despite the additional presence of dyes in the bulk of the separation medium. The dyes are thus able to indicate the presence and locations of proteins or polypeptides in the separation medium without the need for removing unassociated dyes from the medium.