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1.发明授权GRAM POSITIVE BACTERIAL CELLS COMPRISING A DISRUPTED FLAGELLIN GENE, FLAGELLIN-BASED FUSION PROTEINS AND USE IN REMOVAL OF METAL IONS FROM A LIQUID 有权革兰氏阳性细菌中含有BROKEN FLAGELLINGEN，融合蛋白ON FLAGELLINBASIS及其用于除去金属离子从液体
公开(公告)号：EP1836221B1
公开(公告)日：2011-08-03
申请号：EP05822486.6
申请日：2005-12-02
申请人： CSIR
CPC分类号： C07K14/31 , C02F3/34 , C02F2101/20 , C07K2319/00
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公开(公告)号：EP2235045B1
公开(公告)日：2012-10-03
申请号：EP08870024.0
申请日：2008-12-08
申请人： CSIR
发明人： BERGER, Eldie , DU PLESSIS, Erika, Margarete , LOUW, Maureen, Elizabeth , CRAMPTON, Michael, Craig , GERBER, Isak, Bartholomeus
CPC分类号： C07K14/32 , C07K2319/00
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公开(公告)号：EP2356246A1
公开(公告)日：2011-08-17
申请号：EP08875859.4
申请日：2008-11-17
申请人： CSIR
发明人： VISSER, Daniel, Finsch , GORDON, Gregory, Ernest, Robert , HENNESSY, Fritha , RASHAMUSE, Konanani, Justice , BRADY, Dean , BODE, Moira, Leanne , LOUW, Maureen, Elizabeth
CPC分类号： C12P19/385 , C12N9/1077
摘要： A method for manipulating a nucleoside includes converting guanosine to 5- methyluridine by means of biocatalytic transglycosylation. The biocatalyst comprises a combination of a purine nucleoside phosphorylase from Bacillus halodurans, PNPase (BH1531 ) of sequence ID No 1, or a protein with at least 90% sequence similarity thereto, and a pyhmidine nucleoside phosphorylase (PyNPase).
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公开(公告)号：EP2356246B1
公开(公告)日：2012-08-22
申请号：EP08875859.4
申请日：2008-11-17
申请人： CSIR
发明人： VISSER, Daniel, Finsch , GORDON, Gregory, Ernest, Robert , HENNESSY, Fritha , RASHAMUSE, Konanani, Justice , BRADY, Dean , BODE, Moira, Leanne , LOUW, Maureen, Elizabeth
CPC分类号： C12P19/385 , C12N9/1077
摘要： A method for manipulating a nucleoside includes converting guanosine to 5- methyluridine by means of biocatalytic transglycosylation. The biocatalyst comprises a combination of a purine nucleoside phosphorylase from Bacillus halodurans, PNPase (BH1531 ) of sequence ID No 1, or a protein with at least 90% sequence similarity thereto, and a pyhmidine nucleoside phosphorylase (PyNPase).
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公开(公告)号：EP2235045A1
公开(公告)日：2010-10-06
申请号：EP08870024.0
申请日：2008-12-08
申请人： CSIR
发明人： BERGER, Eldie , DU PLESSIS, Erika, Margarete , LOUW, Maureen, Elizabeth , CRAMPTON, Michael, Craig , GERBER, Isak, Bartholomeus
CPC分类号： C07K14/32 , C07K2319/00
摘要： A method of producing a flagellin-based chimeric protein includes culturing a B. halodurans BhFD05 (Δhag, ΔfIiD, ΔwprA, ΔaIp, Δapr, Δvpr, Δasp) strain deposited under Accession Number 41533 at the NCIMB. The strain is caused to express and secrete high levels of a flagellin-based chimeric protein into an extracellular growth medium. The flagellin-based chimeric protein comprises a heterologous peptide (i) inserted in-frame into a flagellin variable region which is flanked on its N-terminal side by an N-terminal fragment of a flagellin polypeptide and, optionally, flanked on its C-terminal side by a C- terminal fragment of a flagellin polypeptide, or (ii) fused to the C-terminal of a flagellin polypeptide.
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6.发明公开GRAM POSITIVE BACTERIAL CELLS COMPRISING A DISRUPTED FLAGELLIN GENE, FLAGELLIN-BASED FUSION PROTEINS AND USE IN REMOVAL OF METAL IONS FROM A LIQUID 有权革兰氏阳性细菌中含有BROKEN FLAGELLINGEN，融合蛋白ON FLAGELLINBASIS及其用于除去金属离子从液体
公开(公告)号：EP1836221A2
公开(公告)日：2007-09-26
申请号：EP05822486.6
申请日：2005-12-02
申请人： CSIR
CPC分类号： C07K14/31 , C02F3/34 , C02F2101/20 , C07K2319/00
摘要： The invention provides f lagellin-based fusion proteins (FBFP) useful for a variety of purposes, in bioremediation to remove metal ions from a liquid, to express an enzyme or an immunogen, nucleic acids encoding the FBFP, vectors containing the nucleic acids, and host cells harboring the vectors. Furthermore, the invention provides methods for obtaining over-expression and surface display of heterologous polypeptides in Gram-positive bacterial cells in Bacillus halodurans in particular. In addition, the invention features gene-disrupted bacteria useful for expressing the recombinant FBFP on their surfaces. Also included in the invention are genetic constructs useful for making FBFP and methods of using the FBFP.
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