-
1.发明公开
公开(公告)号：EP1166120A1
公开(公告)日：2002-01-02
申请号：EP00917985.4
申请日：2000-03-15
发明人： SIIMAN, Olavi , MATIJEVIC, Egon , SONDI, Ivan
IPC分类号： G01N33/58 , G01N33/533 , G01N33/543
CPC分类号： B82Y15/00 , G01N33/588 , Y10T428/2982 , Y10T428/2991
摘要： A semiconductor nanoparticle for use in analysis of biological samples is described. This semiconductor nanoparticle is composed of an aminodextran which is bound to at least one nanoparticle of the formula (X Y)n wherein X is selected from the group comprising Cd?2+, Hg2+¿, and Zn2+ and combinations thereof; and Y is selected from the group comprising S?2-, Se2- and Te2-¿ and combinations thereof; and n = approximately 50 to 1000. Also provided are methods of making these semiconductor nanoparticles and methods of making conjugates composed of these semiconductor nanoparticles linked to ligands. Also described are uses for the conjugates in a variety of biological assays.
-
2.发明授权BIODEGRADABLE PARTICLE COATINGS HAVING A PROTEIN COVALENTLY IMMOBILIZED BY A CROSSLINKING AGENT 失效与生物可降解粒子份通过网络SAY固定的蛋白质
公开(公告)号：EP0556346B1
公开(公告)日：2001-07-11
申请号：EP92904811.4
申请日：1991-10-25
IPC分类号： G01N33/553
CPC分类号： G01N33/54313 , B03C1/01 , G01N33/531 , G01N33/5434 , G01N33/54393 , G01N2446/20 , G01N2446/30 , G01N2446/84 , G01N2446/86 , G01N2446/90 , Y10T428/2991 , Y10T428/2998
-
3.发明授权PROTEIN-COLLOIDAL METAL-AMINODEXTRAN COATED PARTICLE AND METHODS OF PREPARATION AND USE 有权金属包覆颗粒PROTEINKOLLOIDALEN及其制造方法
公开(公告)号：EP1023602B1
公开(公告)日：2006-01-11
申请号：EP98949745.8
申请日：1998-10-01
发明人： SIIMAN, Olavi , GORDON, Kristie , RODRIGUEZ, Carlos, M. , BURSHTEYN, Alexander , MAPLES, John, A. , WHITESELL, James, Keller
IPC分类号： G01N33/553 , G01N33/543 , G01N33/548 , G01N33/50
CPC分类号： G01N33/5094 , G01N33/54313 , G01N33/54353 , G01N33/54393 , G01N33/548 , G01N33/553 , Y10T428/2982 , Y10T428/2991
摘要： A stable colloidal particle comprises a colloidal-sized core substrate having amine-reactive functional groups thereon with an aminodextran coating over its peripheral surface and a layer of colloidal-sized metallic solid overlaying the aminodextran coating. A linker attached to the metallic solid has a free amino group to which a protein is attached by covalent bonding to the free amino group. Such novel particles are useful in flow cytometry, particularly useful in the simultaneous analyses of subpopulations of leukocytes. Methods of preparation of such particles, as well as methods of use are provided. These conjugates enable the simultaneous analyses of at least two different mutually exclusive subsets of white blood cells, based on the binding affinity of the conjugated protein.
-
4.发明公开PROTEIN-COLLOIDAL METAL-AMINODEXTRAN COATED PARTICLE AND METHODS OF PREPARATION AND USE 有权金属包覆颗粒PROTEINKOLLOIDALEN及其制造方法
公开(公告)号：EP1023602A1
公开(公告)日：2000-08-02
申请号：EP98949745.8
申请日：1998-10-01
发明人： SIIMAN, Olavi , GORDON, Kristie , RODRIGUEZ, Carlos, M. , BURSHTEYN, Alexander , MAPLES, John, A. , WHITESELL, James, Keller
IPC分类号： G01N33/553 , G01N33/543 , G01N33/548 , G01N33/50
CPC分类号： G01N33/5094 , G01N33/54313 , G01N33/54353 , G01N33/54393 , G01N33/548 , G01N33/553 , Y10T428/2982 , Y10T428/2991
摘要： A stable colloidal particle comprises a colloidal-sized core substrate having amine-reactive functional groups thereon with an aminodextran coating over its peripheral surface and a layer of colloidal-sized metallic solid overlaying the aminodextran coating. A linker attached to the metallic solid has a free amino group to which a protein is attached by covalent bonding to the free amino group. Such novel particles are useful in flow cytometry, particularly useful in the simultaneous analyses of subpopulations of leukocytes. Methods of preparation of such particles, as well as methods of use are provided. These conjugates enable the simultaneous analyses of at least two different mutually exclusive subsets of white blood cells, based on the binding affinity of the conjugated protein.
-
5.发明授权
公开(公告)号：EP1166120B1
公开(公告)日：2004-08-04
申请号：EP00917985.4
申请日：2000-03-15
发明人： SIIMAN, Olavi , MATIJEVIC, Egon , SONDI, Ivan
IPC分类号： G01N33/58 , G01N33/533 , G01N33/543
CPC分类号： B82Y15/00 , G01N33/588 , Y10T428/2982 , Y10T428/2991
-
6.发明授权
公开(公告)号：EP1023601B1
公开(公告)日：2004-03-03
申请号：EP98953228.8
申请日：1998-10-01
IPC分类号： G01N33/543
CPC分类号： G01N33/56972 , G01N33/5434 , G01N33/553 , G01N2446/20 , G01N2446/90 , Y10T428/2991 , Y10T428/2998
摘要： A stable protein-coated nickel particle useful in biological assays contains a nickel particle having removed from the surface thereof nickel oxide; a linker attached to said nickel particle, the linker having a free amino group; and a protein attached to said linker by covalently bonding to the free amino group. Methods of producing and using these oxide-free nickel-protein conjugates are disclosed.
-
公开(公告)号：EP1354198A2
公开(公告)日：2003-10-22
申请号：EP02739082.2
申请日：2002-01-02
发明人： SIIMAN, Olavi
IPC分类号： G01N33/543 , G01N33/566
CPC分类号： G01N33/54313 , G01N33/54306 , G01N2800/52 , Y10S435/968
摘要： A method of analyzing the concentration of soluble analyte in a sample involves performing a competition assay using a predetermined amount of formed bodies to which are attached at least one analyte, varying known concentrations of an unlabeled ligand that binds to said analyte, and a known concentration of said ligand labeled with a detectable marker. After analysis in a flow cytometer, the test sample and a plurality of control samples generate data used for determining the concentration of the soluble analyte in the sample.
-
8.发明公开
公开(公告)号：EP1023601A2
公开(公告)日：2000-08-02
申请号：EP98953228.8
申请日：1998-10-01
IPC分类号： G01N33/543
CPC分类号： G01N33/56972 , G01N33/5434 , G01N33/553 , G01N2446/20 , G01N2446/90 , Y10T428/2991 , Y10T428/2998
摘要： A stable protein-coated nickel particle useful in biological assays contains a nickel particle having removed from the surface thereof nickel oxide; a linker attached to said nickel particle, the linker having a free amino group; and a protein attached to said linker by covalently bonding to the free amino group. Methods of producing and using these oxide-free nickel-protein conjugates are disclosed.
-
9.发明公开
公开(公告)号：EP0981747A1
公开(公告)日：2000-03-01
申请号：EP98922261.7
申请日：1998-05-14
发明人： SIIMAN, Olavi , BURSHTEYN, Alexander , MYLVAGANAM, Ravindra , RAYNOR, Robert , ROTH, Patricia , SMITH, Cecilia , WILKINSON, Julie
IPC分类号： G01N33/533 , G01N33/566 , G01N33/569
CPC分类号： G01N33/533 , G01N33/566 , G01N33/56977 , Y10S436/80 , Y10S436/805
摘要： Ligand-aminodextran-(phycobiliprotein or tandem dye) conjugates useful for detection of a desired target biological material by providing an enhanced fluorescent signal are described. Also described is a method for a single-measurement quantification of multiple populations of cells based upon the labeling of different pairs of cell populations, each pair containing mutually exclusive cell receptors which are expressed at substantially similar receptor densities with labeled ligands for each receptor. One cell population is labeled with a ligand capable of binding to a first cell surface receptor which ligand is directly conjugated to a fluorescent phycobiliprotein or tandem dye; and a second cell population is labeled with a ligand capable of binding to a second cell surface receptor, which ligand is cross-linked by an aminodextran to a fluorescent phycobiliprotein or tandem dye.
-
10.发明授权
公开(公告)号：EP0981747B1
公开(公告)日：2002-07-31
申请号：EP98922261.7
申请日：1998-05-14
发明人： SIIMAN, Olavi , BURSHTEYN, Alexander , MYLVAGANAM, Ravindra , RAYNOR, Robert , ROTH, Patricia , SMITH, Cecilia , WILKINSON, Julie
IPC分类号： G01N33/533 , G01N33/566 , G01N33/569
CPC分类号： G01N33/533 , G01N33/566 , G01N33/56977 , Y10S436/80 , Y10S436/805
-
-
-
-
-
-
-
-
-
搜索结果
10 条记录 (耗时 0.016 秒)
