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1.发明公开Method for producing useful substances using genetically engineered viral DNA 失效使用遗传工程病毒DNA生产有用物质的方法
公开(公告)号：EP0175852A3
公开(公告)日：1987-09-16
申请号：EP85107711
申请日：1985-06-21
CPC分类号： C12N15/86 , C07K14/56 , C12N2710/14143
摘要： A method of producing useful substances which comprising propagating in cultured cells or in a host a recombinant Bombyx mori nuclear polyhedrosis virus (BmNPV) DNA is disclosed. The BmNPV DNA is produced by recombination with a double-stranded DNA containing (i) a 5'- upstream BmNPV DNA fragment originally occurring upstream from the structural gene coding for the production of polyhedral protein and also including the promotor region for the structural gene, (ii) a translational start codon and (iii) a gene coding for the production of a useful substance exogenous to the virus, with or without (iv) a 3'-downstream BmNPV DNA fragment originally occurring downstream from the structural gene coding for the production of polyhedral protein.
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2.发明授权Method for producing useful substances using genetically engineered viral DNA 失效使用遗传工程病毒DNA生产有用物质的方法
公开(公告)号：EP0175852B1
公开(公告)日：1992-01-15
申请号：EP85107711.5
申请日：1985-06-21
CPC分类号： C12N15/86 , C07K14/56 , C12N2710/14143
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公开(公告)号：EP0222412A3
公开(公告)日：1987-09-09
申请号：EP86115833
申请日：1986-11-14
发明人： Maeda, Susumu , Furusawa, Mitsuru , Marumoto, Yasumasa , Horiuchi, Tadashi , Sato, Yoshinari , Saeki, Yoshiyuki
CPC分类号： C12N15/86 , C07K14/005 , C07K14/56 , C07K14/65 , C07K2319/00 , C07K2319/09 , C07K2319/735 , C07K2319/75 , C12N2710/14122 , C12N2710/14143
摘要： Recombinant Bombyx mon nuclear polyhedrosis viruses having, in the polyhedral protein-encoding structural gene portion of the Bombyx mon nuclear polyhedrosis virus DNA (BmNPV DNA), a structural gene for a desired protein as joined to the whole of or a part of the polyhedral protein-encoding structural gene with or without interposition of a linker base sequence are provided. By propagating the above viruses in silkworm-derived cells or silkworms, fused proteins of a desired protein and the whole of or a part of the polyhedral protein as joined together with or without interposition of a linking amino acid or peptide are produced. The desired proteins can be obtained from the fused proteins by cleavage or decomposition at the linking site.
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4.发明公开Method for producing useful substances using genetically engineered viral DNA 失效一种用于通过使用基因工程改造的病毒DNA的生产有用的物质的过程。
公开(公告)号：EP0175852A2
公开(公告)日：1986-04-02
申请号：EP85107711.5
申请日：1985-06-21
CPC分类号： C12N15/86 , C07K14/56 , C12N2710/14143
摘要： A method of producing useful substances which comprising propagating in cultured cells or in a host a recombinant Bombyx mori nuclear polyhedrosis virus (BmNPV) DNA is disclosed. The BmNPV DNA is produced by recombination with a double-stranded DNA containing (i) a 5'- upstream BmNPV DNA fragment originally occurring upstream from the structural gene coding for the production of polyhedral protein and also including the promotor region for the structural gene, (ii) a translational start codon and (iii) a gene coding for the production of a useful substance exogenous to the virus, with or without (iv) a 3'-downstream BmNPV DNA fragment originally occurring downstream from the structural gene coding for the production of polyhedral protein.
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