公开(公告)号：EP2094831B1

公开(公告)日：2015-09-16

申请号：EP07869044.3

申请日：2007-12-07

申请人： Dow AgroSciences LLC

发明人： LIRA, Justin M. ,  WRIGHT, Terry R. ,  RUSSELL, Sean M. ,  MERLO, Donald J. ,  WEBB, Steven Robert ,  ARNOLD, Nicole L. ,  ROBINSON, Andrew E. ,  SMITH, Kelley A.

IPC分类号： C12N1/21 ,  C12N15/82 ,  A01H1/00

CPC分类号： C12N15/821 ,  C12N15/8275

公开(公告)号：EP2094831A2

公开(公告)日：2009-09-02

申请号：EP07869044.3

申请日：2007-12-07

申请人： Dow AgroSciences LLC

发明人： LIRA, Justin M. ,  WRIGHT, Terry R. ,  RUSSELL, Sean M. ,  MERLO, Donald J. ,  WEBB, Steven Robert ,  ARNOLD, Nicole L. ,  ROBINSON, Andrew E. ,  SMITH, Kelley A.

IPC分类号： C12N1/21 ,  C12N15/82 ,  A01H1/00

CPC分类号： C12N15/821 ,  C12N15/8275

摘要： The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

公开(公告)号：EP2597943B1

公开(公告)日：2018-02-07

申请号：EP11813293.5

申请日：2011-07-29

申请人： Dow AgroSciences LLC

发明人： MERLO, Donald J. ,  RUSSELL, Sean M. ,  RETALLACK, Diane ,  WOOSLEY, Aaron ,  MEADE, Tom ,  NARVA, Kenneth E.

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/84 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12N15/8205 ,  C07K14/195 ,  C07K14/325 ,  C12N15/74 ,  C12N15/743 ,  C12N15/8274 ,  C12N15/8286 ,  Y02A40/162

摘要： Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.

公开(公告)号：EP2597943A2

公开(公告)日：2013-06-05

申请号：EP11813293.5

申请日：2011-07-29

申请人： Dow AgroSciences LLC

发明人： MERLO, Donald J. ,  RUSSELL, Sean M. ,  RETALLACK, Diane ,  WOOSLEY, Aaron ,  MEADE, Tom ,  NARVA, Kenneth E.

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/84 ,  C12N1/21

CPC分类号： C12N15/8205 ,  C07K14/195 ,  C07K14/325 ,  C12N15/74 ,  C12N15/743 ,  C12N15/8274 ,  C12N15/8286 ,  Y02A40/162

摘要： Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.