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    Novel herbicide resistance genes
    4.
    发明授权
    Novel herbicide resistance genes 有权
    Neue Herbizidresistenz-Gene

    公开(公告)号：EP1740039B1

    公开(公告)日：2012-06-27

    申请号：EP05771746.4

    申请日：2005-05-02

    申请人： Dow AgroSciences, LLC

    发明人： WRIGHT, Terry, R. ,  LIRA, Justin, M. ,  MERLO, Donald, J. ,  ARNOLD, Nicole L.

    IPC分类号： C12N15/53 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N15/8274 ,  A01N37/38 ,  A01N43/60 ,  A01N57/20 ,  C12N9/0069 ,  C12N9/0071 ,  C12N15/8209 ,  C12N15/8241 ,  C12N15/8247 ,  C12N15/8249 ,  C12N15/8255 ,  C12N15/8261 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8275 ,  C12N15/8282 ,  C12N15/8283 ,  C12N15/8286

    摘要： The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or "stacked" together with another herbicide resistance gene, preferably a glyphosate. resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No ±-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

    摘要翻译： 本发明提供了不仅对2,4-D和其它苯氧基生长素除草剂具有抗性的新型植物，而且还涉及芳氧基苯氧基丙酸酯除草剂。 迄今为止，没有任何期望或建议，通过引入单个基因可以产生具有这两个这些有利性质的植物。 本发明还包括单独产生一种或多种本发明的酶或与其它除草剂抗性基因，优选草甘膦一起“堆叠”的植物。 抗性基因，以提供更广泛和更强大的杂草控制，增加的治疗灵活性和改进的除草剂抗性管理选择。 更具体地，根据本发明使用的优选的酶和基因在本文中被称为AAD（芳氧基链烷酸酯加氧酶）基因和蛋白质。 以前据报道，没有使用β-酮戊二酸依赖性双加氧酶具有降解不同化学类别和作用方式的除草剂的能力。 这种高度新颖的发现是显着的除草剂耐受性作物性状机会的基础以及可选择标记技术的发展。 本发明还包括控制杂草的相关方法。 本发明能够以新的方式使用除草剂的新型组合。 此外，本发明提供了防止形成和控制对一种或多种除草剂（例如草甘膦）具有抗性（或天然更耐受）的杂草的新方法。

    NOVEL SELECTABLE MARKER GENES
    6.
    发明公开
    NOVEL SELECTABLE MARKER GENES 有权
    新型选择标记基因

    公开(公告)号：EP2094831A2

    公开(公告)日：2009-09-02

    申请号：EP07869044.3

    申请日：2007-12-07

    申请人： Dow AgroSciences LLC

    发明人： LIRA, Justin M. ,  WRIGHT, Terry R. ,  RUSSELL, Sean M. ,  MERLO, Donald J. ,  WEBB, Steven Robert ,  ARNOLD, Nicole L. ,  ROBINSON, Andrew E. ,  SMITH, Kelley A.

    IPC分类号： C12N1/21 ,  C12N15/82 ,  A01H1/00

    CPC分类号： C12N15/821 ,  C12N15/8275

    摘要： The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.